Exercise Metabolism
ABAZAR Teimoori; Alireza Ruzbahani; ZAhra Karimi mehr
Abstract
Aim:. The purpose of this study was to investigate The effect of eight weeks of increasing resistance training and BCAA nanoliposome supplementation on MiR-200a, HSP60, soleus muscle mitochondrial gene expression and serum IGF-1 in aged male rats.Methods: 32 old male were randomly divided into 4 groups: ...
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Aim:. The purpose of this study was to investigate The effect of eight weeks of increasing resistance training and BCAA nanoliposome supplementation on MiR-200a, HSP60, soleus muscle mitochondrial gene expression and serum IGF-1 in aged male rats.Methods: 32 old male were randomly divided into 4 groups: control, supplement (BCAA), combined (resistant training + BCAA) and exercise. Resistance training consisted of eight weeks of ladder training with moderate intensity (70% of MVCC) and five days a week. Rats in the supplement and combined groups received BCAA nanoliposome supplement at the rate of 600 mg per kilogram of body weight by gavage 5 days a week for 8 weeks. MiR200a and HSP60 were obtained using real-time PCR method and serum IGF-1 was obtained using ELISA method. Statistical analysis was performed with two-way, one-way analysis of variance and Tukey's post hoc test.Finding: significant decrease in MiR-200a and a increase in the expression of the HSP60 gene and serum IGF-1 of rats in the combination and exercise group compared to the supplement and control groups (p=0.001)., No significant difference was observed between the control and supplement groups (p=0.105).Conclusion: The direct effect of miR-200a and HSP60 on IGF-1 signal has an important effect on muscle growth and atrophy. The increase of IGF-1 as a result of strength training and BCAA supplementation causes hypertrophy and creates an anabolic environment and can affect age-related muscle growth factors and can bring benefits in this regard for the elderly.Keywords: Resistance training, BCAA, micro RNA, HSP60.
Exercise Physiology
Aghil Mahdavi; Roghayeh Pouzesh Jadidi; Karim Azali Alamdari
Abstract
Introduction: The aim of this study was to evaluate the effects of high-intensity interval training (HIIT) and curcumin supplementation on cardiac heat shock proteins level and gene expression level of microRNAs in rats exposed to arsenic.Methods: 32 male Wistar rats were randomly divided into four groups ...
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Introduction: The aim of this study was to evaluate the effects of high-intensity interval training (HIIT) and curcumin supplementation on cardiac heat shock proteins level and gene expression level of microRNAs in rats exposed to arsenic.Methods: 32 male Wistar rats were randomly divided into four groups of Control, Curcumin, Exercise and concomitant(Curcumin+Exercise). Arsenic 5 mg/kg.day and curcumin 15 mg/kg.day, were consumed orally for entire the study period. HIIT were conducted for six weeks (5 d/w, 60 min/session (consisted of 10 intervals of running (4 min) at 85-90% of Vo2max with 2 min recovery at 50-60% of Vo2max). Tissue protein content and gene expression levels was assessed by Western blotting and PCR methods respectively. Results: No between group difference was observed for gene expression levels of miR-21(P>0.05).The cardiac HSP60, HSP20 levels and the gene expression levels of miR-30 was significantly higher in all three intervention groups compared to Control group(P<0.001). Moreover, the cardiac HSP60, HSP20 content was significantly higher in concomitant group compared to both groups of Exercise and Curcumin(P<0.05). Conclusion: Arsenic exposure may likely increase the vulnerability of cardiomyocytes to injury by altering the amount of heat shock proteins content and the expression levels of microRNAs. Both HIIT and curcumin supplementation could be likely effective to reduce the hazards of arsenic within heart, However, the concomitant intervention might be had more beneficial effects. However, more research is still needed to be done due to the lack of similar evidence and no comprehensive measurement of the influential factors.
