نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 استادیار گروه تربیت بدنی، واحد نیشابور، دانشگاه ازاد اسلامی، نیشابور، ایران،
2 استاد گروه فیزیولوژی ورزشی، دانشگاه ازاد اسلامی، واحد تهران مرکزی، تهران، ایران،
3 دانشیار گروه فیزیولوژی ورزشی، دانشگاه ازاد اسلامی، واحد تهران مرکزی، تهران، ایران،
4 کارشناسی ارشد، مربی، آموزش و پرورش ناحیه ٥ مشهد، خراسان رضوی، ایران،
5 دکتری فیزیولوژی ورزش، مربی، اموزش و پرورش ناحیه ١ مشهد، خراسان رضوی، ایران
چکیده
هدف: با این که چاقی در تمام دنیا فراگیر شده است تلاش برای کاهش این اپیدمیک موثر نبوده است. بنابراین نیاز به یافتن راه های جدید پیشگیری از چاقی وجود دارد. هدف از انجام این پژوهش تعیین اثر تمرین استقامتی و آدنوزین بر بیان ژن A1AR در بافت چربی احشایی رت های نر چاق بود.
روش‌شناسی: بیست سر رت نر صحرایی، نژاد ویستار به مدت دوازده هفته با غذای پر چرب تغذیه شدند تا به میانگین وزنی 30±319 گرم رسیدند. پس از همسان سازی، رت ها به صورت تصادفی در چهار گروه کنترل-سالین، کنترل-آدنوزین، تمرین-آدنوزین و تمرین-سالین تقسیم شدند. برنامه تمرینی شامل دویدن روی نوارگردان بدون شیب به مدت 12 هفته بود. هر هفته پنج جلسه با سرعت 25-20متر در دقیقه و به مدت31-15 دقیقه اجرا شد. برای اندازه‌گیری بیان نسبی ژن A1AR از روش PCR استفاده شد. اطلاعات جمع آوری شده با استفاده از روش آماری آنالیز واریانس دو طرفه مورد بررسی قرار گرفت.
یافته‌ها: هر دو متغیر تمرین استقامتی و مصرف آدنوزین (به‌طور مستقل) کاهش معنی داری را در تغییرات بیان ژن A1AR بافت چربی احشایی نشان دادند (001/0= P). همچنین اثرات تعاملی تمرین و آدنوزین حاکی از کاهش معنی‌دار در تغییرات بیان نسبی ژن A1AR بود (003/0=P)
نتیجه‌گیری: تمرینات استقامتی و مصرف آدنوزین به عنوان آنتاگونیست های A1AR ، محرک‌های قوی لیپولیز بافت چربی هستند و این پتانسیل را دارند که به عنوان عوامل لیپولیتیک موثر در چاقی به کار گرفته شوند.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of endurance training and adenosine consumption on the a1ar gene expression in the visceral adipose tissue of obese male rats
نویسندگان [English]
- A Barjaste Yazdi 1
- MA Azarbayjani 2
- H Matin Homaee 3
- M Peeri 2
- F Torabi 4
- Z Ramezani 5
1 Assistant Professor, Department of Exercise Physiology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
2 Professor, Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
3 Associate Professor, Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
4 Master's in Physical Education, Instructor, Department of Education, District 5, Mashhad, Khorassan-e-Razavi, Iran
5 PhD in Sport Physiology, Instructor, Department of Education, District 1, Mashhad, Khorassan-e-Razavi, Iran
چکیده [English]
Aim: Although obesity is becoming epidemic all around the world, the effort to reduce its prevalence has not been effective. Thus, there is a need to find further paths to prevent obesity. The aim of this study was to determine the effect of endurance training and adenosine on the A1AR gene expression in the visceral adipose tissue of obese male rats
Method: Twenty wistar strain male rats were fed with high-fat food for twelve weeks and reached the average weight of 319+-30 grams. The rats were divided randomly into four groups after being conformed. The exercise training session included running on the treadmill with no slope for 12 week. Each week, five sessions were held for 15-31 minutes with the speed of 20-25 meters per minute. In order to measure the relative gene expression of A1AR, the PCR method was used. The data was analyzed using the statistical method of two-way analysis of variance.
Results: both variable of endurance training and adenosine consumption (independently) demonstrated a significant decrease in the A1AR gene expression in the visceral adipose tissue. (P=0.001). Also, the intraction of training and adenosine affects significant reduction of the A1AR expression.
Conclusion: Endurance training and adenosine are strong stimulants of adipose tissue lipolysis as A1AR antagonists and have the potential to be used as effective lipolysis agents in obesity
Keywords: Endurance Training, A1AR, Visceral White Adipose Tissue.
کلیدواژه‌ها [English]
- Keywords: Endurance Training
- A1AR
- Visceral White Adipose Tissue
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