نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I

نویسندگان

1 دانشکده تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران

2 گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه گیلان، رشت، ایران

3 گروه فیزولوژی ورزش، دانشکده علوم ورزشی، دانشگاه گیلان

چکیده

هدف: هدف از پژوهش حاضر بررسی اثر فعالیت ورزشی هوازی با و بدون BFR بر روی پروتئین‌های داینامیک و متیوفاژی میتوکندری عضلات اسکلتی انسانی است.
روش شناسی: در مطالعه مقطعی متقاطع، 5 مرد جوان (سن: 30/2±40/33، وزن: 49/10±64/79، شاخص توده بدنی: 27/2±24/26) دو مداخله‌ فعالیت ورزشی هوازی با BFR (EX+BFR) و بدون BFR (EX) را در دو روز مجزا اجرا کردند. فعالیت ورزشی هوازی شامل 5 وهله فعالیت 2 دقیقه‌ای با سرعت 50 متر بر دقیقه و 1 دقیقه استراحت بین هر وهله بود که با و بدون BFR اجرا شد. برای اندازه‌گیری مقادیر پروتئینی MFN2 و DRP1، BCL2 و Parkin عضله اسکلتی از روش وسترن بلات استفاده شد. تجزیه و تحلیل داده ها با استفاده از آزمون t همبسته در سطح معنی داری 05/0 انجام شد.
یافته‌ها: نتایج نشان داد که هر دو EX + BF و EX به طور قابل توجهی باعث افزایش MFN2 و پارکین و همچنین کاهش DRP1 را نسبت به قبل از آزمون شد (05/0p <). با این حال، EX + BF باعث افزایش سطح پروتئین BCL2 در مقایسه با پیش آزمون (05/0p <) شد. همچنین، EX + BF اثر معنی داری بر افزایش MFN2، BCL2 و پارکین و کاهش DRP1 در مقایسه با EX دارد (05/0p <).
نتیجه‌گیری: بر اساس یافته‌های این مطالعه، به نظر می‌رسد که فعالیت هوازی با و بدون BFR محرک قوی برای بهبود داینامیک میتوکندری و فرایند میتوفاژی عضله اسکلتی باشد.

کلیدواژه‌ها

عنوان مقاله [English]

investigate the effect of aerobic exercise with and without BFR on mitochondrial dynamic (MFN2 &DRP1) and mitophagy proteins (PARKIN &BCL2) of human skeletal muscle

نویسندگان [English]

  • ali aryashakib 1
  • Bahman Mirzaei 2
  • payam saidie 3

1 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran

2 Department of Exercise Physiology, faculty of exercise sciences, University of Guilan, Rasht, Iran

3 Department of Exercise physiology, faculty of exercise sciences, university of guilan

چکیده [English]

The purpose of this study was to investigate the effect of aerobic exercise with and without BFR on mitochondrial dynamic and mitophagy proteins of human skeletal muscle. In a cross-sectional study, five young men (age: 33.4 ± 2.30 year, weight: 79.64 ± 10.49 kg, BMI: 26.24 ± 2.27kg/cm2) performed two aerobic exercise intervention with BFR (EX+BFR) and without BFR (EX) in two separate days. The aerobic exercise included 5 bouts of 2-min activities, interspersed by 1 min active rest which performed with and without BFR. Western Blot method was used to measure the protein levels of MFN2, DRP1, BCL2 and Parkin of skeletal muscle. Data analysis was performed using T test at a significant level of 0.05. The results of the data analyze showed that both EX+BF and EX significantly increased MFN2 and Parkin and also significantly decreased DRP1 compared to the pre-test (p < 0.05). However, EX+BF resulted in a significant increase protein levels of BCL2 compared to the pre-test (p < 0.05). Also, EX+BF has a significant effect on MFN2, BCL2 and Parkin elevation and DRP1 decrease in compared to EX (p < 0.05). Based on the findings of this study, it seems that aerobic exercise with and without BFR is a strong stimulant for the improvement of mitochondrial dynamics and mitophagy of skeletal muscle.

کلیدواژه‌ها [English]

  • BFR
  • MFN2
  • DRP1
  • BCL2
  • Parkin
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