fatemeh bozorgi; Siroos choobineh
Abstract
Introduction: The aim of the present research was to compare the effects of four weeks of aerobic exercise (swimming) and resistance exercise (climbing stairs) on the levels of Sirtuin-1 ...
Read More
Introduction: The aim of the present research was to compare the effects of four weeks of aerobic exercise (swimming) and resistance exercise (climbing stairs) on the levels of Sirtuin-1 protein (SIRT1) and Nicotinamide Adenine Dinucleotide (NAD+) in the brain in the chronic experimental autoimmune encephalomyelitis (EAE) period. Methodology: This study was conducted using a laboratory method. 40 female C57BL6 mice, 8 weeks old with an average weight of 18±2 grams, were selected for the experiment. For the experiment process, the sample group members were divided into four groups: healthy control, EAE, EAE + swimming, and EAE + resistance exercise. After inducing EAE with MOG35-33, the exercise groups performed swimming and resistance exercise programs for 30 minutes, 5 days a week for 4 weeks. On the 30th day post-induction (chronic disease period), the mice were anesthetized with ketamine and xylazine, followed by brain dissection and tissue sampling. The levels of SIRT1 and NAD+ proteins were measured using immunohistochemistry. One-way analysis of variance (ANOVA) was used to compare the research groups. Tukey's post hoc test was used to compare differences between groups. Results: The findings of the current research showed that both swimming and resistance exercises significantly increased the levels of NAD+ and SIRT1 proteins compared to the EAE group, although resistance exercise was more effective than swimming (P ≤ 0.05). Conclusion: Exercise, especially resistance exercise, may reduce disease progression in EAE by increasing NAD+ and SIRT1 levels.
