University of Guilan Metabolism and Exercise 2322-2867 5 1 2015 05 22 Action of L-Arginin on oxidative- nitrosative stress induced by acute exercise in liver of rats Action of L-Arginin on oxidative- nitrosative stress induced by acute exercise in liver of rats 1 9 1690 FA Masume Kazemi Sayad Mohmmad Marandi Associate professor, University of Isfahan Ahmand Movahedian Attar professor, Isfahan University.of Medical Sciences Hosin Mohammadian PhD student in Pharmaceutical Biotechnology Hamid Reza Sharifi Jebeli Veterinary Technician Journal Article 2015 04 05 <strong>Aim: </strong>The aim of the present investigation is evaluate the effect of L- Arginine supplements on the oxidative and nitrosative stress in liver of rats following acute exercise on a treadmill<strong>.</strong> <br /><strong>Method:</strong> Thirty two male Vistar rats (160-210 g) were randomly divided into four groups: control (C); L-Arg treatment (Arg); exhaustive exercise (E); and exhaustive exercise with L-Arg treatment (E + Arg). E and E+Arg groups performed a 1 hour acute running test, or until exhaustion on a treadmill (16-26 m/min) and Arg and E+Arg groups treated orally with the L-Arg (2% diet, for 30 days). Sampling was performed 1 hour after exercise. Nitric oxide production was evaluated by measuring nitrite formation, using Griess reagent. oxidant-Antioxidant’s ratio was measured as TOS/ TAC levels. <br /><strong>Results</strong>: According to the means of the oxidant-antioxidant’s balance (E)=0.91±0.12, (SC+Arg)=0.92 0.04 and (E+Arg)=0.91±0.09, TOS/TAC decreased in (E), (SC+Arg) and (E+Arg) groups compared with control group (SC); however, this decrease was not significant. And the results showed nitric oxide in (SC) group (mean=9.01 6.31 ) was more than other groups, but there was no significant change in there. <br /><strong>Conclusion:</strong> The results of the present study didn’t show existence of oxidative-nitrosative stress and supplementation effect in muscle 1 h after acute exercise. <strong>Aim: </strong>The aim of the present investigation is evaluate the effect of L- Arginine supplements on the oxidative and nitrosative stress in liver of rats following acute exercise on a treadmill<strong>.</strong> <br /><strong>Method:</strong> Thirty two male Vistar rats (160-210 g) were randomly divided into four groups: control (C); L-Arg treatment (Arg); exhaustive exercise (E); and exhaustive exercise with L-Arg treatment (E + Arg). E and E+Arg groups performed a 1 hour acute running test, or until exhaustion on a treadmill (16-26 m/min) and Arg and E+Arg groups treated orally with the L-Arg (2% diet, for 30 days). Sampling was performed 1 hour after exercise. Nitric oxide production was evaluated by measuring nitrite formation, using Griess reagent. oxidant-Antioxidant’s ratio was measured as TOS/ TAC levels. <br /><strong>Results</strong>: According to the means of the oxidant-antioxidant’s balance (E)=0.91±0.12, (SC+Arg)=0.92 0.04 and (E+Arg)=0.91±0.09, TOS/TAC decreased in (E), (SC+Arg) and (E+Arg) groups compared with control group (SC); however, this decrease was not significant. And the results showed nitric oxide in (SC) group (mean=9.01 6.31 ) was more than other groups, but there was no significant change in there. <br /><strong>Conclusion:</strong> The results of the present study didn’t show existence of oxidative-nitrosative stress and supplementation effect in muscle 1 h after acute exercise. https://jme.guilan.ac.ir/article_1690_752fccb5a81865a93c60f004e69579af.pdf