University of Guilan Metabolism and Exercise 2322-2867 7 2 2017 11 22 The effect of 6 weeks high intensity interval training (HIIT) on expression of PTP-1B in muscle tissue in type 2 diabetic rats The effect of 6 weeks high intensity interval training (HIIT) on expression of PTP-1B in muscle tissue in type 2 diabetic rats 125 134 3379 10.22124/jme.2017.3379 FA F Soheili Msc Student in Exercise Physiology M Azizi Assistant Professor, University of Razi R Soori Associate Professor, University of Tehran V Tahmasebi Assistant Professor, University of Razi Journal Article 2018 01 15 Aim: Insulin resistance plays an important role in the development of abnormalities such as impaired glucose tolerance, type2 diabetes, obesity, and hyperlipidemia. The discovery of protein tyrosine phosphatase 1b (PTP-1B) seems to be a milestone in the investigation of insulin signaling transmission. Thus the purpose of this study was to evaluate the effect of 6 weeks of high intensity interval training (HIIT) on expression of PTP-1B in the muscle tissue in type 2 diabetic rats.. Method: In this study, 20, ten week old diabetic male rats were randomly divided into high intensity interval and control groups. The training group performed training protocol on a treadmill for 6 weeks and 5 session/week, with gradual increase in speed (25-35 m/min) during scheduled runs. Forty-eight hours after the last training session blood and tissue (from gastrocnemius) sampling were carried out in diabetic rats and PTP-1B gene expression was evaluated by Rea-Time PCR method. Data was analyzed using independent t-test and Shapiro Wilk at a significant level of p≤0/05. Results: Findings show that PTP-1B was decreased in the diabetic rats of HIIT group but was not significant (P=0.432). Also, insulin resistance index and fasting glucose significantly were decreased in HIIT group (P=0.001), but serum insulin was increased (P=0.001). Conclusion: According to the results, it seems that HIIT does not affect PTP-1B gene expression, but it improves type 2 diabetes and insulin resistance. Keywords: PTP-1B gene expression, type 2 diabetes, high intensity interval training, insulin resistance. Aim: Insulin resistance plays an important role in the development of abnormalities such as impaired glucose tolerance, type2 diabetes, obesity, and hyperlipidemia. The discovery of protein tyrosine phosphatase 1b (PTP-1B) seems to be a milestone in the investigation of insulin signaling transmission. Thus the purpose of this study was to evaluate the effect of 6 weeks of high intensity interval training (HIIT) on expression of PTP-1B in the muscle tissue in type 2 diabetic rats.. Method: In this study, 20, ten week old diabetic male rats were randomly divided into high intensity interval and control groups. The training group performed training protocol on a treadmill for 6 weeks and 5 session/week, with gradual increase in speed (25-35 m/min) during scheduled runs. Forty-eight hours after the last training session blood and tissue (from gastrocnemius) sampling were carried out in diabetic rats and PTP-1B gene expression was evaluated by Rea-Time PCR method. Data was analyzed using independent t-test and Shapiro Wilk at a significant level of p≤0/05. Results: Findings show that PTP-1B was decreased in the diabetic rats of HIIT group but was not significant (P=0.432). Also, insulin resistance index and fasting glucose significantly were decreased in HIIT group (P=0.001), but serum insulin was increased (P=0.001). Conclusion: According to the results, it seems that HIIT does not affect PTP-1B gene expression, but it improves type 2 diabetes and insulin resistance. Keywords: PTP-1B gene expression, type 2 diabetes, high intensity interval training, insulin resistance.

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