University of Guilan Metabolism and Exercise 2322-2867 14 1 2024 04 20 Comparing the effect of resistance training and swimming on the levels of sirtuin-1 and nicotinamide dinucleotide in the brains of rats with experimental autoimmune encephalomyelitis. Comparing the effect of resistance training and swimming on the levels of sirtuin-1 and nicotinamide dinucleotide in the brains of rats with experimental autoimmune encephalomyelitis. 28 41 7794 10.22124/jme.2024.27077.355 FA Fatemeh Bozorgi Kish International Campus of Tehran University Siroos Choobineh Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran Journal Article 2024 03 23 Introduction: The aim of the present research was to compare the effects of four weeks of aerobic exercise (swimming) and resistance exercise (climbing stairs) on the levels of Sirtuin-1 protein (SIRT1) and Nicotinamide Adenine Dinucleotide (NAD+) in the brain in the chronic experimental autoimmune encephalomyelitis (EAE) period. Methodology: This study was conducted using a laboratory method. 40 female C57BL6 mice, 8 weeks old with an average weight of 18±2 grams, were selected for the experiment. For the experiment process, the sample group members were divided into four groups: healthy control, EAE, EAE + swimming, and EAE + resistance exercise. After inducing EAE with MOG35-33, the exercise groups performed swimming and resistance exercise programs for 30 minutes, 5 days a week for 4 weeks. On the 30th day post-induction (chronic disease period), the mice were anesthetized with ketamine and xylazine, followed by brain dissection and tissue sampling. The levels of SIRT1 and NAD+ proteins were measured using immunohistochemistry. One-way analysis of variance (ANOVA) was used to compare the research groups. Tukey's post hoc test was used to compare differences between groups. Results: The findings of the current research showed that both swimming and resistance exercises significantly increased the levels of NAD+ and SIRT1 proteins compared to the EAE group, although resistance exercise was more effective than swimming (P ≤ 0.05). Conclusion: Exercise, especially resistance exercise, may reduce disease progression in EAE by increasing NAD+ and SIRT1 levels. Introduction: The aim of the present research was to compare the effects of four weeks of aerobic exercise (swimming) and resistance exercise (climbing stairs) on the levels of Sirtuin-1 protein (SIRT1) and Nicotinamide Adenine Dinucleotide (NAD+) in the brain in the chronic experimental autoimmune encephalomyelitis (EAE) period. Methodology: This study was conducted using a laboratory method. 40 female C57BL6 mice, 8 weeks old with an average weight of 18±2 grams, were selected for the experiment. For the experiment process, the sample group members were divided into four groups: healthy control, EAE, EAE + swimming, and EAE + resistance exercise. After inducing EAE with MOG35-33, the exercise groups performed swimming and resistance exercise programs for 30 minutes, 5 days a week for 4 weeks. On the 30th day post-induction (chronic disease period), the mice were anesthetized with ketamine and xylazine, followed by brain dissection and tissue sampling. The levels of SIRT1 and NAD+ proteins were measured using immunohistochemistry. One-way analysis of variance (ANOVA) was used to compare the research groups. Tukey's post hoc test was used to compare differences between groups. Results: The findings of the current research showed that both swimming and resistance exercises significantly increased the levels of NAD+ and SIRT1 proteins compared to the EAE group, although resistance exercise was more effective than swimming (P ≤ 0.05). Conclusion: Exercise, especially resistance exercise, may reduce disease progression in EAE by increasing NAD+ and SIRT1 levels.

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