University of Guilan Metabolism and Exercise 2322-2867 Articles in Press 2026 05 17 The effect of eight weeks of interval training on thymosin beta4(Tβ4) plasma levels and blood pressure indices in middle-aged men with hypertension The effect of eight weeks of interval training on thymosin beta4(Tβ4) plasma levels and blood pressure indices in middle-aged men with hypertension 9611 10.22124/jme.2026.31753.429 FA Mehdi Bostani Department of physical education, Ahv.C., Islamic Azad University, Ahvaz, Iran. Mehdi Borhan Department of Physical Education, Ahv.C., Islamic Azad University, Ahvaz, Iran Journal Article 2025 09 22 The aim of the present study was to investigate the effect of eight weeks of interval training on plasma thymosin beta-4 levels and blood pressure indices in middle-aged men with hypertension. 32 middle-aged men with an age range of 50-60 years and high blood pressure were purposefully selected and then randomly assigned to two control (n=15) and experimental (n=17) groups. The experimental group performed interval training for eight weeks, three sessions per week, at an intensity of 50-75% of heart rate reserve. Plasma thymosin beta-4 levels were measured 48 hours before and after the training period using the ELISA protein assay. Systolic blood pressure, diastolic blood pressure, and mean arterial blood pressure were also measured in the subjects of the two groups before and after the training period. Analysis of covariance (ANCoVA) was also used to compare variables in the two groups. Data were analyzed at a significance level of 0.05 using SPSS-23 software. The results showed that eight weeks of interval training resulted in a significant increase in thymosin beta-4 (P < 0.05). Also, a significant decrease was observed in systolic blood pressure, diastolic blood pressure, and mean arterial blood pressure after eight weeks of interval training (P < 0.05). According to the results of the present study, it is recommended that people with high blood pressure use interval training in their daily routine to control blood pressure and thereby reduce its complications. The aim of the present study was to investigate the effect of eight weeks of interval training on plasma thymosin beta-4 levels and blood pressure indices in middle-aged men with hypertension. 32 middle-aged men with an age range of 50-60 years and high blood pressure were purposefully selected and then randomly assigned to two control (n=15) and experimental (n=17) groups. The experimental group performed interval training for eight weeks, three sessions per week, at an intensity of 50-75% of heart rate reserve. Plasma thymosin beta-4 levels were measured 48 hours before and after the training period using the ELISA protein assay. Systolic blood pressure, diastolic blood pressure, and mean arterial blood pressure were also measured in the subjects of the two groups before and after the training period. Analysis of covariance (ANCoVA) was also used to compare variables in the two groups. Data were analyzed at a significance level of 0.05 using SPSS-23 software. The results showed that eight weeks of interval training resulted in a significant increase in thymosin beta-4 (P < 0.05). Also, a significant decrease was observed in systolic blood pressure, diastolic blood pressure, and mean arterial blood pressure after eight weeks of interval training (P < 0.05). According to the results of the present study, it is recommended that people with high blood pressure use interval training in their daily routine to control blood pressure and thereby reduce its complications.