F Soheili; M Azizi; R Soori; V Tahmasebi
Abstract
Aim: Insulin resistance plays an important role in the development of abnormalities such as impaired glucose tolerance, type2 diabetes, obesity, and hyperlipidemia. The discovery ...
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Aim: Insulin resistance plays an important role in the development of abnormalities such as impaired glucose tolerance, type2 diabetes, obesity, and hyperlipidemia. The discovery of protein tyrosine phosphatase 1b (PTP-1B) seems to be a milestone in the investigation of insulin signaling transmission. Thus the purpose of this study was to evaluate the effect of 6 weeks of high intensity interval training (HIIT) on expression of PTP-1B in the muscle tissue in type 2 diabetic rats.. Method: In this study, 20, ten week old diabetic male rats were randomly divided into high intensity interval and control groups. The training group performed training protocol on a treadmill for 6 weeks and 5 session/week, with gradual increase in speed (25-35 m/min) during scheduled runs. Forty-eight hours after the last training session blood and tissue (from gastrocnemius) sampling were carried out in diabetic rats and PTP-1B gene expression was evaluated by Rea-Time PCR method. Data was analyzed using independent t-test and Shapiro Wilk at a significant level of p≤0/05. Results: Findings show that PTP-1B was decreased in the diabetic rats of HIIT group but was not significant (P=0.432). Also, insulin resistance index and fasting glucose significantly were decreased in HIIT group (P=0.001), but serum insulin was increased (P=0.001). Conclusion: According to the results, it seems that HIIT does not affect PTP-1B gene expression, but it improves type 2 diabetes and insulin resistance. Keywords: PTP-1B gene expression, type 2 diabetes, high intensity interval training, insulin resistance.
