A Fazeli Sani; hasan Matinhomaee; A Banaeifar
Abstract
Aim: The aim of the present study was to investigate the effect of 4 weeks of aerobic exercise on cognitive function and mitochondrial dynamics in the hippocampal tissue of male Wistar rats with Alzheimer's disease.Methods: For this purpose, 21 male Wistar rats at 20 months of age were randomly divided ...
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Aim: The aim of the present study was to investigate the effect of 4 weeks of aerobic exercise on cognitive function and mitochondrial dynamics in the hippocampal tissue of male Wistar rats with Alzheimer's disease.Methods: For this purpose, 21 male Wistar rats at 20 months of age were randomly divided into 3 groups: Alzheimer's disease (n = 7), Alzheimer's disease + aerobic exercise (n = 7) and control group (n = 7). Alzheimer's disease was induced by intrahippocampal injection of Aβ42 (1 microliter per side). Seven days after surgery, the exercise group performed 4 weeks of treadmill training (5 days per week at a speed of 10 to 15 m/min). Forty-eight hours after the last training session, the animals underwent behavioral tests. Twenty-four hours after the behavioral test, all rats were killed and hippocampal tissue was extracted. The mRNA expression of OPA1, Mfn2 and Drp1 genes was assayed using Real Time-PCR. One-way analysis of variance was used for statistical analysis.Ethical Considerations: All stages of the study were conducted according to the ethical guidelines and authorization of Research Deputy of Islamic Azad University, Central Tehran Branch No. IR.IAU.TMU.REC.1399.124.Results: The results showed that spatial learning (P ≤ 0.001) and memory performance (P ≤ 0.001) as well as the gene expression of OPA1 (P ≤ 0.001) and Mfn2 (P ≤ 0.001) in animals with Alzheimer's disease decreased compared to the control group, while the gene expression of Drp1 increased (P ≤ 0.001). Aerobic exercise in patient animals improved spatial learning (P ≤ 0.001) and memory performance (P ≤ 0.001), increased hippocampal OPA1 (P ≤ 0.001) and Mfn2 (P ≤ 0.001) genes expression, and decreased Drp1 gene expression compared with Alzheimer's disease group (P ≤ 0.001).Conclusion: In general, it seems that aerobic exercise can improve spatial learning and memory performance in Alzheimer's disease by modulating abnormal mitochondrial dynamics.
A Barjaste Yazdi; MA Azarbayjani; H Matin Homaee; M Peeri; F Torabi; Z Ramezani
Abstract
Aim: Although obesity is becoming epidemic all around the world, the effort to reduce its prevalence has not been effective. Thus, there is a need to find further paths to prevent obesity. The aim of this study was to determine the effect of endurance training and adenosine on the A1AR gene expression ...
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Aim: Although obesity is becoming epidemic all around the world, the effort to reduce its prevalence has not been effective. Thus, there is a need to find further paths to prevent obesity. The aim of this study was to determine the effect of endurance training and adenosine on the A1AR gene expression in the visceral adipose tissue of obese male rats Method: Twenty wistar strain male rats were fed with high-fat food for twelve weeks and reached the average weight of 319+-30 grams. The rats were divided randomly into four groups after being conformed. The exercise training session included running on the treadmill with no slope for 12 week. Each week, five sessions were held for 15-31 minutes with the speed of 20-25 meters per minute. In order to measure the relative gene expression of A1AR, the PCR method was used. The data was analyzed using the statistical method of two-way analysis of variance. Results: both variable of endurance training and adenosine consumption (independently) demonstrated a significant decrease in the A1AR gene expression in the visceral adipose tissue. (P=0.001). Also, the intraction of training and adenosine affects significant reduction of the A1AR expression. Conclusion: Endurance training and adenosine are strong stimulants of adipose tissue lipolysis as A1AR antagonists and have the potential to be used as effective lipolysis agents in obesity Keywords: Endurance Training, A1AR, Visceral White Adipose Tissue.
