Document Type : Research Paper I Open Access I Released under (CC BY-NC) license


1 Associate professor, University of Isfahan

2 professor, Isfahan University.of Medical Sciences

3 PhD student in Pharmaceutical Biotechnology

4 Veterinary Technician


Aim: The aim of the present investigation is evaluate the effect of L- Arginine supplements on the oxidative and nitrosative stress in liver of rats following acute exercise on a treadmill.
Method: Thirty two male Vistar rats (160-210 g) were randomly divided into four groups: control (C); L-Arg treatment (Arg); exhaustive exercise (E); and exhaustive exercise with L-Arg treatment (E + Arg). E and E+Arg groups performed a 1 hour acute running test, or until exhaustion on a treadmill (16-26 m/min) and Arg and E+Arg groups treated orally with the L-Arg (2% diet, for 30 days). Sampling was performed 1 hour after exercise. Nitric oxide production was evaluated by measuring nitrite formation, using Griess reagent. oxidant-Antioxidant’s ratio was measured as TOS/ TAC levels.
Results: According to the means of the oxidant-antioxidant’s balance (E)=0.91±0.12, (SC+Arg)=0.92 0.04 and (E+Arg)=0.91±0.09, TOS/TAC decreased in (E), (SC+Arg) and (E+Arg) groups compared with control group (SC); however, this decrease was not significant. And the results showed nitric oxide in (SC) group (mean=9.01 6.31 ) was more than other groups, but there was no significant change in there.
Conclusion: The results of the present study didn’t show existence of oxidative-nitrosative stress and supplementation effect in muscle 1 h after acute exercise.