Aim: Due to improved Anaerobic performance following creatine or caffeine supplementation and ambiguity and contradiction regarding consecutive effect of creatine and caffeine supplementation, the purpose of this study was to examine the effect of creatine and caffeine consecutive supplementation on lactate and adrenaline, cortisol, insulin like growth factor (IGF-1) hormones response to anaerobic test in young female handball players.
Method: 30 club female handball players were randomly assigned to 4 groups: placebo, creatine (Cr group consumed 20 g of creatine, 4 doses 5 g per day for 7 days), caffeine (capsule containing 6 mg/kg Caff) and creatine-caffeine (Cr group consumed 20 g of creatine, 4 doses 5 g per day for 7 days and capsule containing 6 mg/kg Caff). 50 minutes following ingestion of the capsules (Caff/Placebo), 5ml blood sample obtained from brachial vein subjects to measure aforementioned hormones and values recorded as pre-test. Then, subjects performed the RAST protocol. Again, blood samplings were taken immediately following the protocol and as post-test values were recorded.
Results: Changes of plasma levels of lactate in creatine group at posttest was higher than the control group (P<0.05), but there was no difference in serum levels of cortisol between intervention groups and serum levels of adrenaline in caffeine group at posttest was higher than the control group. Also, serum levels of IGF-1 in caffeine group was lower than the control group. Moreover, fatigue index and maximum power in caffeine group were higher than the control group and compared to the other groups (P<0.05).
Conclusion: Consecutive consumption of creatine and caffeine on lactate and anabolic-catabolic hormones response likely had not ergogenic effect and only lactate response in creatine group and hormonal changes and anaerobic performance in caffeine group were significant.