Document Type : Research Paper I Open Access I Released under (CC BY-NC) license


1 MSc student in exercise physiology

2 Assitant Professor, Ayatollah Amoli Branch, Islamic Azad University

3 Assitant Professor, Gilan Univercity


Aim: Lipocalin-2 and interleukin-1 beta (IL-1ß) are two inflammatory markers affecting on insulin resistance. The aim of this study was to investigate the effect of aerobic training with cinnamon extract on blood levels of Lipocalin-2 and IL-1ß in insulin-resistant rats.
Method: : In this experimental study thirty-six Wistar male rats were randomly divided into four groups: control (n=9), aerobic training (n=9), cinnamon (n=9), and aerobic training+cinnamon (n=9). Insulin-resistance status induced by %10 fructose solutions during 5 weeks. Training groups subjected to a 5-day per week aerobic training program (with 75-80% VO2max) for 8 weeks. Supplementayion groups subjected to injected 200 ml/kg/day cinnamon extract.
Results: Exercise training significantly reduced Lipocalin-2 compared to the control group, and compared to extract group (P<0.05). Also, in all three experimental groups, IL-1 beta decreased significantly compared to control (P<0.05), and significant decrease was observed in the extract group compared to the training group and extract (PConclusion: Results of this study indicated that aerobic training with cinnamon extract more than any of them alone effects on reduction IL-1ß. But the effect of the combination of aerobic training and cinnamon extract on lipocalin-2 requires further investigation.


  1. Abbassi Daloii A, Fani F, Abdi A. (2016). The Effect of 8 weeks endurance training and L-NAME on Apelin in myocardial tissue and glucose elderly male’s rats. Razi Journal of Medical Sciences. 23(145):22-9.
  2. Abdul            Belotto M, Magdalon J, Rodrigues H, Vinolo M, Curi R, Pithon‐Curi T, et al. (2010). Moderate exercise improves leucocyte function and decreases inflammation in diabetes. Clinical and Experimental Immunology.162(2):237-43.
  3. Agarwal D, Welsch MA, Keller JN, Francis J. (2011). Chronic exercise modulates RAS components and improves balance between pro-and anti-inflammatory cytokines in the brain of SHR. Basic Research in Cardiology.106(6):1069-85.
  4. Almeida HGGD, Campos JJB, Kfouri C, Tanita MT, Dias AE, Souza M. (2002). Profile of patients with diabetes type 1: insulinotherapy and self-monitoring. Revista da Associação Médica Brasileira. 48(2):151-5.
  5. Arora E, Shenoy S, Sandhu J. (2009). Effects of resistance training on metabolic profile of adults with type 2 diabetes. Indian Journal of Medicine Research. 129:515-9.
  6. Atashak S, Ahmadi-Zad A. (2017). Effect of eight weeks of resistance exercise on new biomarkers of cardiovascular disease in obese adult males. Feyz Journal of Kashan University of Medical Sciences.21(3):256-64.
  7. Baum M, Klöpping-Menke K, Müller-Steinhardt M, Liesen H, Kirchner H. (1999). Increased concentrations of interleukin 1-β in whole blood cultures supernatants after 12 weeks of moderate endurance exercise. European Journal of Applied Physiology and Occupational Physiology.79(6):500-3.
  8. Bonora E, Brangani C, Pichiri I. (2008). Abdominal obesity and diabetes. Giornale Italiano Di Cardiologia.9(4):40S-53S.
  9. Cao H, Urban JF, Anderson RA.(2008). Cinnamon polyphenol extract affects immune responses by regulating anti-and proinflammatory and glucose transporter gene expression in mouse macrophages. The Journal of Nutrition.138(5):833-40.

10. Chao LK, Hua KF, Hsu HY, Cheng SS, Lin IF, Chen CJ, et al. (2008). Cinnamaldehyde inhibits pro-inflammatory cytokines secretion from monocytes/macrophages through suppression of intracellular signaling. Food and Chemical Toxicology.46(1):220-31.

11. Choi K, Kim T, Yoo H, Lee K, Cho G, Hwang T, et al. (2009). Effect of exercise training on A‐FABP, lipocalin‐2 and RBP4 levels in obese women. Clinical Endocrinology.70(4):569-74.

12. Conde J, Otero M, Scotece M, Abella V, López V, Pino J, et al. (2016). E74‐like factor 3 and nuclear factor‐κB regulate lipocalin‐2 expression in chondrocytes. The Journal of Physiology.594(21):6133-46.

13. Das UN. (2004). Anti-inflammatory nature of exercise. Nutrition.;20(3):323.

14. Esteve E, Ricart W, Fernández-Real JM. (2009). Adipocytokines and insulin resistance. Diabetes Care.;32(suppl 2):S362-S7.

15. Fathi R, Aslani moghanjoughi S, Talebi Garakani E, Safarzadeh A, Seyghal H. (2015). Effect of 8-week resistance training on plasma visfatin levels and its relation to insulin resistance in insulin-resistant male rats. Iranian Journal of Diabetes and Lipid Disorders.14(6):390-8.

16. Flo TH, Smith KD, Sato S, Rodriguez DJ. (2004). Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron. Nature.432(7019):917.

17. Gheibi N, Parvizi M, Jahani Hashemi H. (2005). The effect of cinnamon on glucose concentration of diabetic rats in presence or absence of insulin. Journal of Qazvin University of Medical Sciences. 9 (3) :3-8

18. Giannopoulou I, Fernhall B, Carhart R, Weinstock RS, Baynard T, Figueroa A, et al. (2005).Effects of diet and/or exercise on the adipocytokine and inflammatory cytokine levels of postmenopausal women with type 2 diabetes. Metabolism.54(7):866-75.

19. Goldhammer E, Tanchilevitch A, Maor I, Beniamini Y, Rosenschein U, Sagiv M. (2005). Exercise training modulates cytokines activity in coronary heart disease patients. International Journal of Cardiology.100(1):93-9.

20. Hagenlocher Y, Hösel A, Bischoff SC, Lorentz A. (2016). Cinnamon extract reduces symptoms, inflammatory mediators and mast cell markers in murine IL-10 colitis. The Journal of Nutritional Biochemistry.30:85-92.

21. Hasanvand B, Soori R, Abbasian S, Rastegar Moghaddam Mansoori M. (2017). The effect of three-week intensive interval training on lipocalin-2 and interleukin1-β in healthy and adult rat Hippocampus. Arak Medical University Journal.20(1):24-34.

22. Hosseini M, Bambaeichi E, Sarir H, kargarfard M, Mogharnasi M. (2018). The effect of six weeks of continuous training with ziziphus jujube extract consumption on lipocalin-2 and adiponectin levels in plasma and heart tissue of rats with myocardial infraction. Quarterly of Horizon of Medical Sciences.24(3):193-202.

23. Hosseini M, Shemshaki A, Saghebjoo M, Gharari Arefi R. (2016). Effect of aerobic training and pistacia atlantica extract consumption on plasma levels of lipocalin-2 and insulin resistance index in streptozotocin-induced diabetic rats. The Horizon of Medical Sciences.22(1):27-33.

24. Jayaraman A, Roberts KA, Yoon J, Yarmush DM, Duan X, Lee K, et al. (2005). Identification of neutrophil gelatinase‐associated lipocalin (NGAL) as a discriminatory marker of the hepatocyte‐secreted protein response to IL‐1β: a proteomic analysis. Biotechnology and Bioengineering.91(4):502-15.

25. Kralisch S, Weise S, Sommer G, Lipfert J, Lossner U, Bluher M, et al. (2009). Interleukin-1ß induces the novel adipokine chemerin in adipocytes in vitro. Regulatory Peptides.154(1):102-6.

26. Kwon HK, Hwang JS, Lee CG, So JS, Sahoo A, Im CR, et al. (2011). Cinnamon extract suppresses experimental colitis through modulation of antigen-presenting cells. World Journal of Gastroenterology: WJG.17(8):976.

27. Lovatel GA, Elsner VR, Bertoldi K, Vanzella C, dos Santos Moysés F, Vizuete A, et al. (2013). Treadmill exercise induces age-related changes in aversive memory, neuroinflammatory and epigenetic processes in the rat hippocampus. Neurobiology of Learning and Memory.101:94-102.

28. Mehrabani J, Damirchi A, Rahmaninia F. (2014). Effect of two aerobic exercise intensity on lipocalin-2, interleukin-1ß levels, and insulin resistance index in sedentary obese men. Sport Physiology.6(21):95-108.

29. Modaresi M, Messripour M, Rajaei R. (2010). Effect of cinnamon extract on the number of spermatocyte and spermatozoa cells in mice. Iranian Journal of Medicinal and Aromatic Plants.26(1):83-90.

30. Mohammadi Domiyeh A, Khajehlandi A. (2012). The effects of 8 eight weeks resistance versus endurance training on lipocalin-2 level in non-athlete male students. Armaghane Danesh.17(5):460-8.

31. Nikooie A, Sedaghat Boroujeni L. (2014). A review of pharmacological properties and functional of cinnamon. Journal of Herbal Drugs.5(3):127-35.

32. Pedersen B. (2007). IL-6 signalling in exercise and disease. Portland Press Limited.

33. Petersen AMW, Pedersen BK. (2005). The anti-inflammatory effect of exercise. Journal of Applied Physiology.98(4):1154-62.

34. Shemshaki A, Hosseini M, Saghebjoo M, Arefi R. (2016). The effect of 6 weeks of aerobic training on plasma levels of lipocalin-2, insulin and insulin resistance in streptozotocin-induced diabetic male rats. Sport Physiology and Management Investigation. 8(1):51-61.

35. Sommer G, Weise S, Kralisch S, Lossner U, Bluher M, Stumvoll M, et al. (2009). Lipocalin‐2 is induced by interleukin‐1β in murine adipocytes in vitro. Journal of Cellular Biochemistry.106(1):103-8.

36. Speisman RB, Kumar A, Rani A, Foster TC, Ormerod BK. (2013). Daily exercise improves memory,stimulates hippocampal neurogenesis and modulates immune and neuroimmune cytokines in aging rats. Brain, behavior, and immunity.28:25-43.

37. Vassilakopoulos T, Karatza M-H, Katsaounou P, Kollintza A, Zakynthinos S, Roussos C. (2003).Antioxidants attenuate the plasma cytokine response to exercise in humans. Journal of Applied Physiology.94(3):1025-32.

38. Wang Y, Lam KS, Kraegen EW, Sweeney G, Zhang J, Tso AW, et al. (2007).Lipocalin-2 is an inflammatory marker closely associated with obesity, insulin resistance, and hyperglycemia in humans. Clinical Chemistry.53(1):34-41.

39. Ward Gm, Wideman L. (2006). The effects of lifestyle activity modification (lam) or a structured exericse program on non-traditional cardiovascular disease (cvd) risk factors in African-American women: The University Of North Carolina At Greensboro.