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Effects of HIIT and curcumin supplementation on plasma CK18 and hepatic GSH level in rats exposed to arsenic

Document Type : Research Paper I Open Access I Released under (CC BY-NC) license

Authors

1 PHD student of Exercise Physiology, Department of Physical Education, Tabriz branch, Islamic Azad University, Tabriz, Iran

2 : Assistant Prof., Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran

3 : Associate Professor of Exercise Physiology, Azarbaijan Shahid Madani University, Tabriz, Iran,

4 : Associate Prof., Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz

5 Assistant Prof., Department of Vet, Tabriz branch, Islamic Azad University

Abstract

Aim: we investigated the effects of six weeks of HIIT and curcumin supplementation on hepatic cellular death and also GSH level in male rats exposed to drinking water arsenic.
Methods: 48 rats were randomized in six groups including HIIT, curcumin, HIIT+curcumin, arsenic, ethanol control and normal control. Arsenic 5 mg/bw.day and curcumin 15 mg/bw.day, were consumed orally for entire the study period. HIIT were conducted for six weeks (5 d/w, 60 min/session (with 4 min running at 85-90% of Vo2max and 2 min recovery at 50-60% of Vo2max intervals).
Results: Arsenic exposure down regulated hepatic CK18 M65 level and also in a lesser extent; the cellular GSH content. Curcumin supplementation either alone or concomitantly with HIIT, could reverse the changes in hepatic CK18 M65 level. However; only in the HIIT+curcumin group, a remarkable elevation of hepatic GSH level was observed.
Conclusion: Arsenic exposure renders liver to an increased oxidative stress and apoptosis level and the both HIIT and curcumin supplementation have appreciable effects to neutralize these hazardous effects. Moreover, only the synergistic effect of HIIT and curcumin supplementation could attenuate the liver antioxidants following arsenic exposure. However, more investigations remains to be done because of the study limitations and lack of similar evidence in this area.

Keywords

Main Subjects

Publisher: University oF Guilan          Copyright © The Authors

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

References
  1. Santra A, Chowdhury A, Ghatak S, Biswas A, Dhali GK. Arsenic induces apoptosis in mouse liver is mitochondria dependent and is abrogated by N-acetylcysteine. Toxicology and Applied Pharmacology. 2007;220(2):146-55.
  2. Muthumani M, Prabu SM. Silibinin potentially protects arsenic-induced oxidative hepatic dysfunction in rats. Toxicology mechanisms and methods. 2012;22(4):277-88.
  3. Jomova K, Jenisova Z, Feszterova M, Baros S, Liska J, Hudecova D, et al. Arsenic: toxicity, oxidative stress and human disease. Journal of Applied Toxicology. 2011;31(2):95-107.
  4. Liu L, Trimarchi JR, Navarro P, Blasco MA, Keefe DL. Oxidative stress contributes to arsenic-induced telomere attrition, chromosome instability, and apoptosis. Journal of Biological Chemistry. 2003;278(34):31998-2004.
  5. Bashir S, Sharma Y, Irshad M, Nag T, Tiwari M, Kabra M, et al. Arsenic induced apoptosis in rat liver following repeated 60 days exposure. Toxicology. 2006;217(1):63-70.
  6. Majumdar S, Karmakar S, Maiti A, Choudhury M, Ghosh A, Das AS, et al. Arsenic-induced hepatic mitochondrial toxicity in rats and its amelioration by dietary phosphate. Environmental Toxicology and Pharmacology. 2011;31(1):107-18.
  7. Rana T, Bera AK, Das S, Bhattacharya D, Pan D, Bandyopadhyay S, et al. Mushroom lectin protects arsenic induced apoptosis in hepatocytes of rodents. Human and Experimental Toxicology. 2011;30(4):307.
  8. Horton A, Fairhurst S, Bus JSJCCRiT. Lipid peroxidation and mechanisms of toxicity. 1987;18(1):27-79.
  9. Chiş I, Mureşan A, Oros A, Nagy A, Clichici SJAPH. Protective effects of Quercetin and chronic moderate exercise (training) against oxidative stress in the liver tissue of streptozotocin-induced diabetic rats. 2016;103(1):49-64.
  10. Georis B, Cardenas A, Buchet JP, Lauwerys R. Inorganic arsenic methylation by rat tissue slices. Toxicology. 1990;63(1):73-84.
  11. Buchet JP, Lauwerys R. Role of thiols in the in-vitro methylation of inorganic arsenic by rat liver cytosol. Biochemical Pharmacology. 1988;37(16):3149-53.
  12. Gyurasics Á, Varga F, Gregus Z. Glutathione-dependent biliary excretion of arsenic. Biochemical Pharmacology. 1991;42(3):465-8.

 

  1. Muthumani M, Prabu SM. Arsenic Induced Oxidative Stress and Its Possible Reversal by Chelation Therapy. Research & Reviews: A Journal of Toxicology. 2019;2(2):16-37.
  2. Itokawa H, Shi Q, Akiyama T, Morris-Natschke SL, Lee K-H. Recent advances in the investigation of curcuminoids. Chinese Medicine. 2008;3:11.
  3. Yadav A, Lomash V, Samim M, Flora SJS. Curcumin encapsulated in chitosan nanoparticles: A novel strategy for the treatment of arsenic toxicity. Chemico-Biological Interactions. 2012;199(1):49-61.
  4. Mathews VV, Binu P, Sauganth Paul MV, Abhilash M, Manju A, Nair RH. Hepatoprotective efficacy of curcumin against arsenic trioxide toxicity. Asian Pacific Journal of Tropical Biomedicine. 2012;2(2, Supplement):S706-S11.
  5. Dadban Shahamat M, Dabidi Roshan V, Farazmandfar T. Effect of Continuous Aerobic Exercise on Bax/Bcl-2 Ratio and Doxorubicin-induced Liver Toxicity in Aging model Rats. Journal of Mazandaran University of Medical Sciences. 2018;28(165):36-46.
  6. Sadat-Hoseini SK, Dabidi Roshan V. Is sub-chronic exercise in Combination with medicinal nanoparticles a protective strategy against Doxorubicin-induced Hepatic oxidative stress and apoptosis in aging model rats? Nanomedicine Journal. 2017;4(4):224-31.
  7. Hinkley JM, Morton AB, Ichinoseki-Sekine N, Huertas AM, Smuder AJ. Exercise Training Prevents Doxorubicin-induced Mitochondrial Dysfunction of the Liver. Medicine and science in sports and exercise. 2019;51(6):1106-15.
  8. Jiang H-K, Wang Y-H, Sun L, He X, Zhao M, Feng Z-H, et al. Aerobic interval training attenuates mitochondrial dysfunction in rats post-myocardial infarction: roles of mitochondrial network dynamics. International journal of molecular sciences. 2014;15(4):5304-22.
  9. Abdul KSM, Jayasinghe SS, Chandana EP, Jayasumana C, De Silva PMC. Arsenic and human health effects: A review. Environmental toxicology and pharmacology. 2015;40(3):828-46.
  10. Ferrario D, Gribaldo L, Hartung T. Arsenic exposure and immunotoxicity: a review including the possible influence of age and sex. Current environmental health reports. 2016;3(1):1-12.
  11. Habib M, Bhatti S, ur Rehman S, Javed N, Shahbaz Aslam M, Shahzad N, et al. Hepatoprotective role of swimming against arsenic induced oxidative stress in mice. Journal of King Saud University - Science. 2019.
  12. Bai J, Yao X, Jiang L, Zhang Q, Guan H, Liu S, et al. Taurine protects against As2O3-induced autophagy in livers of rat offsprings through PPARgamma pathway. Scientific reports. 2016;6:27733.
  13. Eguchi A, Wree A, Feldstein AE. Biomarkers of liver cell death. Journal of Hepatology. 2014;60(5):1063-74.
  14. Schutte B, Henfling M, Kölgen W, Bouman M, Meex S, Leers MP, et al. Keratin 8/18 breakdown and reorganization during apoptosis. Experimental cell research. 2004;297(1):11-26.
  15. Ku N-O, Liao J, Omary MB. Apoptosis generates stable fragments of human type I keratins. Journal of Biological Chemistry. 1997;272(52):33197-203.
  16. Hemmati AA, Olapour S, Varzi HN, Khodayar MJ, Dianat M, Mohammadian B, et al. Ellagic acid protects against arsenic trioxide–induced cardiotoxicity in rat. Human & Experimental Toxicology. 2017;37(4):412-9.
  17. Biswas J, Roy S, Mukherjee S, Sinha D, Roy M