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The effect of nettle extract and aerobic training on the gene expression of VEGF and caspase 3 in mice with melanoma

Document Type : Research Paper I Open Access I Released under (CC BY-NC) license

Authors

1 PHD student,department of sport physiology, ayatollah amoli branch,Islamic azad university , amol

2 department of sport physiology,ayatollah amoli branch,,islamic azad university

3 Associated professor, department of sport physiology, ayatollah amoli branch,Islamic azad university , amol

4 Assistant professor,department of sport physiology, sience and research university,Islamic azad university , tehran.

Abstract

Introduction: The aim of this study was to evaluate the effect of nettle extract consumption and aerobic exercise on VEGF and caspase 3 gene expression in mice with melanoma.
Methodology: In this study, 20 adult male rats with age of 8 week were randomly divided into 4 groups including: control, exercise, extract and exercise + extract. The training program consisted of 30 minutes of running on a treadmill without a slope at a speed of 16 m/min for the first week, and one meter per minute was added every week until it reached 22 meters per minute in the eighth week. One week after melanoma induction, the experimental group consumed 30 mg /kg /day of nettle ethanol extract orally for 8 weeks. RT PCR in blood was used to measure the expression of VEGF and caspase 3 genes.

Results: Data analysis showed that VEGF gene expression was decreased in experimental groups compared to the control group; But did not reach a significant level (p = 0.154). The results also showed that caspase 3 gene expression was significantly increased in the experimental groups compared to the control group (p = 0.000). Post hoc test also showed that caspase 3 gene expression was significantly different between the combination group and other groups.
Conclusion: The results showed that consumption of nettle extract may have anti-cancer effects through various mechanisms such as antioxidant activity, induction of apoptosis inhibition of cell growth and cell migration.

Keywords

Main Subjects

Publisher: University oF Guilan          Copyright © The Authors

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

References
  1. Amjadi F, Javanmard SH, Zarkesh-Esfahani H, Khazaei M, Narimani M. Leptin promotes melanoma tumor growth in mice related to increasing circulating endothelial progenitor cells numbers and plasma NO production. J Exp Clin Cancer Res. 2011; 30: 21
  2. ander Leest RJ, Zoutendijk J, Nijsten T, Mooi WJ, van der Rhee JI, de Vries E, et al. Increasing time trends of thin melanomas in The Netherlands: What are the explanations of recent accelerations? European journal of cancer. 2015;51(18):2833–41.
  3. Balunas, M.J.; Kinghorn, A.D. Drug discovery from medicinal plants. Life Sci. 2005, 78, 431–441.
  4. Bates DO, Hillman NJ, Williams B, Neal CR, Pocock TM. Regulation of microvascular permeability by vascular endothelial growth factors. J Anat (2002) 200(6):581–97.
  5. Caini S, Gandini S, Sera F, Raimondi S, Fargnoli MC, Boniol M, et al. Meta-analysis of risk factors for cutaneous melanoma according to anatomical site and clinico-pathological variant. European journal of cancer. 2009;45(17):3054–63.
  6. Crosby, M. B., Yang, H., Gao, W., Zhang, L., & Grossniklaus, H. E. (2011). Serum vascular endothelial growth factor (VEGF) levels correlate with number and location of micrometastases in a murine model of uveal melanoma. The British journal of ophthalmology, 95(1), 112–117.
  7. dynia G, Grund K, Eckert A, Bock BC, Funke B, MacherGoeppinger S, Sieber S, Herold-Mende C, Wiestler B, Wiestler OD, Roth W. Basal caspase activity promotes migration and invasiveness in glioblastoma cells. Mol Cancer Res. 2007; 5:1232-1240.
  8. Favaloro B, Allocati N, Graziano V, Di Ilio C, De Laurenzi V. Role of apoptosis in disease. Aging (Albany NY). 2012; 4:330-349.
  9. Ferrara N. Molecular and biological properties of vascular endothelial growth factor. J Mol Med (Berl) (1999) 77(7):527–43.
  10. Flanagan L, Meyer M, Fay J, Curry S, Bacon O, Duessmann H, John K, Boland KC, McNamara DA, Kay EW, Bantel H, Schulze-Bergkamen H, Prehn JH. Low levels of caspase-3 predict favourable response to 5FU-based chemotherapy in advanced colorectal cancer: caspase-3 inhibition as a therapeutic approach. Cell Death Dis. 2016; 7:e2087.
  11. Hamilton H, Hall G. Virginia Native Plants. King George, Virginia: Black Cat Press; 2005 Mar. 500 p.
  12. Hanahan, D., & Weinberg, R. A. (2011). Hallmarks of cancer: the next generation. Cell, 144(5), 646–674.
  13. Hirakawa, S., Kodama, S., Kunstfeld, R., Kajiya, K., Brown, L. F., & Detmar, M. (2005). VEGF-A induces tumor and sentinel lymph node lymphangiogenesis and promotes lymphatic metastasis. The Journal of experimental medicine, 201(7), 1089–1099.
  14. Huang S, Mills L, Mian B, Tellez C, McCarty M, Yang XD, et al. Fully humanized neutralizing antibodies to interleukin-8 (ABX-IL8) inhibit angiogenesis, tumor growth, and metastasis of human melanoma. Am J Pathol (2002) 161(1):125–34.
  15. Kandaswami, C.; Kanadaswami, C.; Lee, L.T.; Lee, P.P.; Hwang, J.J.; Ke, F.C.; Huang, Y.T.; Lee, M.T. The antitumor activities of flavonoids. In Vivo 2005, 19, 895–909.
  16. Kim, J. S., Ha, J. Y., Yang, S. J., & Son, J. H. (2018). A Novel Non-Apoptotic Role of Procaspase-3 in the Regulation of Mitochondrial Biogenesis Activators. Journal of cellular biochemistry, 119(1), 347–357.
  17. Koçtürk S, Kayatekin BM, Resmi H, Açıkgöz O, Kaynak C, Özer E. The apoptotic response to strenuous exercise of the gastrocnemius and solues muscle fibers in rats.Eur J ApplPhysiol2008; 102(5):515-524.
  18. Lee, S. D., Shyu, W. C., Cheng, I. S., Kuo, C. H., Chan, Y. S., Lin, Y. M., Tasi, C. Y., Tsai, C. H., Ho, T. J., & Huang, C. Y. (2013). Effects of exercise training on cardiac apoptosis in obese rats. Nutrition, metabolism, and cardiovascular diseases : NMCD, 23(6), 566–573.
  19. Liu YR, Sun B, Zhao XL, Gu Q, Liu ZY, Dong XY, Che N, Mo J. Basal caspase-3 activity promotes migration, invasion, and vasculogenic mimicry formation of melanoma cells. Melanoma Res. 2013; 23:243-253.
  20. Ko¨hler C, Orrenius S, Zhivotovsky B. Evaluation of caspase activity in apoptotic cells. J Immun Methods 2002; 265:97-110.
  21. Masoudzade G, Barari A, Amini S. Synergistic Effect of Aloe vera Extract and Aerobic Training on Tumor Weight and Volume and Levels of Interleukin 6 and Vascular Endothelial Growth Factor in Mice with Breast Cancer. ijbd. 2019; 11 (4) :17-27-
  22. Matthews NH, Li WQ, Qureshi AA, Weinstock MA, Cho E. “Epidemiology of Melanoma”. In: WH Ward and JM Farma, editors. Cutaneous Melanoma: Etiology and Therapy. Brisbane (AU: Codon Publications (2017). The Authors.
  23. McIlwain DR, Berger T, Mak TW. Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol. 2013.
  24. Moller P, Wallin H, Knudsen LE. Oxidative stress associated with exercise, psychological stress and life-style factors. Chemico-biological interactions. 1996 Sep;102(1):17-36.24
  25. Mueller, M. M., & Fusenig, N. E. (2002). Tumor-stroma interactions directing phenotype and progression of epithelial skin tumor cells. Differentiation; research in biological diversity, 70(9-10), 486–4