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The effect of nettle extract and aerobic training on the gene expression of VEGF and caspase 3 in mice with melanoma

Document Type : Research Paper I Open Access I Released under (CC BY-NC) license

Authors

1 PHD student,department of sport physiology, ayatollah amoli branch,Islamic azad university , amol

2 department of sport physiology,ayatollah amoli branch,,islamic azad university

3 Associated professor, department of sport physiology, ayatollah amoli branch,Islamic azad university , amol

4 Assistant professor,department of sport physiology, sience and research university,Islamic azad university , tehran.

Abstract

Introduction: The aim of this study was to evaluate the effect of nettle extract consumption and aerobic exercise on VEGF and caspase 3 gene expression in mice with melanoma.
Methodology: In this study, 20 adult male rats with age of 8 week were randomly divided into 4 groups including: control, exercise, extract and exercise + extract. The training program consisted of 30 minutes of running on a treadmill without a slope at a speed of 16 m/min for the first week, and one meter per minute was added every week until it reached 22 meters per minute in the eighth week. One week after melanoma induction, the experimental group consumed 30 mg /kg /day of nettle ethanol extract orally for 8 weeks. RT PCR in blood was used to measure the expression of VEGF and caspase 3 genes.

Results: Data analysis showed that VEGF gene expression was decreased in experimental groups compared to the control group; But did not reach a significant level (p = 0.154). The results also showed that caspase 3 gene expression was significantly increased in the experimental groups compared to the control group (p = 0.000). Post hoc test also showed that caspase 3 gene expression was significantly different between the combination group and other groups.
Conclusion: The results showed that consumption of nettle extract may have anti-cancer effects through various mechanisms such as antioxidant activity, induction of apoptosis inhibition of cell growth and cell migration.

Keywords

Main Subjects

Publisher: University oF Guilan          Copyright © The Authors

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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Metabolism and Exercise
Volume 11, Issue 1 - Serial Number 1
March 2022
Pages 75-87
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History
  • Receive Date: 23 August 2022
  • Revise Date: 04 September 2022
  • Accept Date: 07 September 2022
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