Document Type : Research Paper I Open Access I Released under (CC BY-NC) license

Authors

• mehran hashemi ¹
• Agha Ali Ghasemnyian ²
• akram karimi asl ³
• samaneh hadi ¹

¹ Master of Applied Sports Physiology Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran.

² Associate professor, Department of Sport Science, Faculty of Humanities, University of Zanjan, Zanjan ,Iran

³ Assistant professor, Department of Sport Sciences, Faculty of Humanities, University of Zanjan, Zanjan, Iran.

Abstract

Background and Aim:The aim of the present study was to investigate the effect of two types of intense training on the levels of thioredoxin reductase-1 enzyme and malondialdehyde in testicular tissue of male wistar rats.

Material and method:19 male Wistar rats,8weeks old and weighing200± 5g, after10 sessions of introducing training protocols and after weighing, were randomly divided into3groups(control, intense endurance training, intense training).Intense endurance training protocol included running on a treadmill for8 weeks and intense periodic training protocol included running on a rodent treadmill for 8 weeks. 48 hours after the last training session,the rats were anesthetized with ether and sacrificed and then the testicular tissue was isolated under sterile conditions.The amount of Thioredoxin Reductase-1 was determined by ELISA method and using a spectrophotometer and also the concentration of Malondialdehyde was determined by three Barbituric acid method using a spectrometer. One-way ANOVA with Tukey's post hoc test was used to analyze the data.

Result:After8 weeks, the amount of Enzyme in the intense endurance training group and intense periodic training group increased significantly compared to the control group(P<0.05). Also,there was no significant change in the amount of Malondialdehyde in testicular tissue of the intense endurance training group and intense periodic training group compared to the control group(P>0.05).

Conclusion: According to the results of this research,it can be said that intense endurance training and intense periodic training has increased the amount of thioredoxin reductase-1 enzyme in a testicular tissue and by creating adaptation in the body's antioxidant system, has not increased malondialdehyde.

Keywords

• Intense endurance training
• Intense periodic training
• Thioredoxin reductase-1 enzyme
• Malondialdehyde
• Testicles

Main Subjects

• Response and adaptation to exercises