Aim: One of the complications of menopause that plays an important role in reducing the quality of life is the reduction of body fat-free mass. The aim of this study was to determine the effect of a period of whole body vibration course on myostatin and follistatin Serum in Postmenopausal Women. Methods: In this semi experimental study, 20 postmenopausal women with hypothyroidism were selected and divided into two groups of 10: control (C) and whole body vibration training (V). Group V performed these exercises for 12 weeks and three times a week, with a frequency of 30 to 50 Hz, applying on upper and lower limb training. At the beginning and end of the study, blood serum myostatin and follistatin levels were measured using the ELISA method, , body fat percentage, body mass index, and body weight. Due to the normality of data distribution, independent t and covariance statistical methods were used for analysis at a significance level of 0.05. Results: 12 weeks of whole body vibration training in group V significantly increased the serum levels of follistatin (P=0.001), and decreased myostatine (P=0.018) compared to the control group. There was also a significant decrease in body fat percentage (P=0.041), body mass index (P=0.044) and weight (P=0.047).Conclusions: It seems that whole body vibration training increases serum levels of follistatin and decreases myostatin in postmenopausal women, and these positive changes may play an important role in improving lean mass of postmenopausal women.