Document Type : Research Paper I Open Access I Released under (CC BY-NC) license
Authors
1 Kish International Campus of Tehran University
2 Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran
Abstract
The aim of this study is to compare the effect of four weeks of aerobic exercise (swimming) and resistance exercise (climbing the ladder) on the levels of sirtuin-1 (SIRT1) and nicotinamide adenine dinucleotide (NAD+) in the chronic course of EAE disease.
Materials and methods: This study was conducted by laboratory method. 40 female C57BL6 mice were selected for the experiment. To implement the testing process, the members of the sample group were divided into four control groups, EAE, swimming and resistance exercise. After induction of EAE with MOG35-33, the exercise groups performed a swimming and resistance training program for 30 minutes, 5 days a week for 4 weeks. On the 30th day after induction (chronic period of the disease), mice were anesthetized with ketamine and xylazine injection, then dissection and tissue sampling were performed. SIRT1 and NAD+ values were measured by immunohistochemistry. One-way analysis of variance (ANOVA) was used to compare research groups. Tukey's post hoc test was used to compare differences between groups.
Finding: The findings of the study showed that swimming and resistance exercise both significantly increased NAD+ and compared to the EAE group. Although, resistance exercise compared to swimming exercise, showed that the first was a more effective method (P ≤ 0.05). The results of Tukey's test showed that the control group presented better results compared to the EAE, swimming and resistance training groups.
Conclusion: Exercise, especially resistance exercise, may reduce disease progression in EAE by increasing NAD+ and SIRT1.
Keywords
- Multiple sclerosis
- Experimental autoimmune encephalomyelitis
- Sirtuin-1
- Nicotinamide adenine dinucleotide
- Exercise
Main Subjects
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