Document Type : Research Paper I Open Access I Released under (CC BY-NC) license
Authors
1 Department of Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran
2 Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran
Abstract
Introduction: Obesity leads to oxidative stress and chronic inflammation. This study aimed to investigate the effects of high-intensity interval training (HIIT) and alternate-day fasting (ADF) on inflammatory markers and oxidative stress in the visceral adipose tissue of rats fed a high-fat, high-fructose diet (HFF).
Methodology: 40 eight-week-old Sprague-Dawley rats were randomly divided into five groups (n=8): control (Con), HFF, HFF+HIIT, HFF+ADF, and HFF+HIIT+ADF. The HIIT protocol was perfomed for 12 weeks, five sessions per week, consisted of seven intervals of four minutes at 85-90% of maximum velocity (Vmax), interspersed with two minutes of active recovery. ADF groups were deprived of food on alternate days. Tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10) protein expression levels were measured using Western blot analysis, and malondialdehyde (MDA) levels were assessed using a calorimetric method. Independent t-tests, one-way analysis of variance (ANOVA), and Tukey's post hoc test were employed to assess differences between groups at a significance level of p<0.05.
Results: The HFF showed a significant increase in weight compared to the CON group. MDA levels and IL-10 and TNF-α protein expression were significantly elevated in the HFF (p<0.05). The HIIT, ADF, and HIIT+ADF comared to the HFF group exhibited significant reductions in weight and MDA levels (p<0.05).
Conclusions: The study indicated that HFF is associated with weight gain, elevated oxidative stress and inflammatory markers. HIIT, ADF, and especially HIIT+ADF reduce body weight and mitigate oxidative stress and modulated inflammation.
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