Document Type : Research Paper I Open Access I Released under (CC BY-NC) license
Authors
- Hadi Golpasandi 1
- Mohammad Rahman Rahimi 2
- Peyman Ghasemi 3
- Slahadin Ahmadi 4
- Siamand Abdollahpour 5
1 Postdoctoral Researcher in exercise Physiology, Department of exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran.
2 Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran.
3 Department of Exercise Physiology, Faculty of Humanities, Department of Exercise Physiology, Tehran, Iran.
4 Department of Physiology and Pharmacology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
5 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran.
Abstract
Introduction: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance and hyperglycemia, often accompanied by dysregulation of lipid metabolism and enhanced autophagic activity. This study examined the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on autophagy-related proteins (Beclin-1, LC3-II, and PLIN-2) in visceral adipose tissue of diabetic rats.
Methodology: Male Wistar rats were divided into normal control (NC), diabetic control (DC), diabetic+HIIT (D+HIIT), and diabetic+MICT (D+MICT) groups. Diabetes was induced through streptozotocin injection and high-fat diet. The exercise program was implemented over a period of 8 weeks . Protein levels were measured by Western blot, and metabolic indices were assessed using standard methods.
Results: Diabetes significantly increased Beclin-1, LC3-II, and PLIN-2 levels. Both HIIT and MICT reduced these levels, with MICT showing stronger effects on Beclin-1 and LC3-II reduction. Both exercise regimens also decreased blood glucose, insulin levels, and insulin resistance.
ConclusionsExercise training, particularly MICT, appears to play a protective role against type 2 diabetes complications by downregulating autophagy proteins. These findings highlight the importance of exercise interventions in diabetes management.
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