Document Type : Research Paper I Open Access I Released under (CC BY-NC) license
Authors
1 Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Associate Professor, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 Associate Professor, Department of Physical Education and Sport Sciences, shahr-e-Qods Branch, Islamic Azad University, Iran.
Abstract
Introduction: The aim of the present study was to investigate the changes and correlation between the antioxidant enzyme superoxide dismutase (SOD) and the expression of the inhibitor of cytokine signaling 3 (SOCS3) gene in rats fed a high-fat diet (HFD) after 6 weeks of aerobic training with cannabis supplementation.
Methodology: In this study, 40 male Wistar rats were divided into 5 groups (8 each): healthy control, high-fat diet (HFD), HFD + aerobic training, +HFD cannabis supplementation (Sup) and HFD+Tr+Sup. After the training intervention for 6 weeks, Cannabidiol Extract was gavage after each training session at a dose of 100ng/kg to the rats in the supplement groups. the liver of the rats was examined with a Zonecare-Q9 ultrasound device. After the training intervention, SOCS3 variable was measured by RealTime PCR and SOD by ELISA. One-way ANOVA and Pearson correlation were used to analyze the data.
Results: The results showed that high-fat diet in the HFD group induced grade 2 fatty liver and increased cholesterol and triglyceride levels (p<0.05). The HFD, HFD+Sup, and HFD+Tr groups had a significant decrease in SOD (p<0.05). The HFD+Tr, HFD+sup, and HFD+Tr+Sup groups showed a significant decrease in SOCS3 compared to the HFD group (p<0.05). Also, the decrease in SOCS3 in the HFD+Tr+Sup group was significant compared to the HFD+Sup and HFD+Tr groups. (p>0.05)
Conclusions: No relationship was found between antioxidant enzymes and SOCS3. However, exercise training and Cannabidiol Extract were able to regulate lipid profiles, SOD, and SOCS3 after a high-fat diet.
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