Document Type : Research Paper I Open Access I Released under (CC BY-NC) license
Author
Assistant Professor of Exercise Physiology, Department of Exercise Physiology, Bon.C. University of Islamic Azad University, Bonab, Iran.
Abstract
Objective: It seems that with increasing age, the speed of contractile proteins and the synthesis of mitochondrial proteins of muscles decreases.The purpose of this study was to investigate the effect of eight weeks of resistance training and creatine-hydrochloride supplementation on myogenin and HSP70 gene expression in the soleus muscle of aged male rats.
Methodology: 32 old male were randomly divided into 4 groups: control, supplement, combined and exercise. Resistance training consisted of eight weeks of ladder training with moderate intensity (70% of MVCC) and five days a week. Rats in the supplement and combined groups received creatine-HCL at the rate of 200 mg/kg of body weight by gavage 5 days a week for 8 weeks. myogenin and HSP70 were obtained using real-time PCR method. Statistical analysis was performed with two-way and Tukey's post hoc test.
Results: The results showed a significant increase in the expression of HSP70 and myogenin gene in the soleus muscle in the combination group compared to the control and supplement groups (p=0.001). No significant difference was observed between the control and supplement groups (p=0.158).
Conclusion: It seems that the increase of HSP70 and the inhibition of muscle apoptosis caused by sarcopenia and the increase of mitochondrial function as well as the increased effect of myogenin on muscle hypertrophy and the creation of an anabolic environment for muscle growth in old age indicate the importance of resistance training and the consumption of protein supplements in old age.
Keywords: Resistance training, creatine-hydrochloride, myogenin, HSP70.
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