Document Type : Research Paper I Open Access I Released under (CC BY-NC) license
Authors
1 MSc Student, Department of Sport Sciences, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Professor of Exercise Physiology, Faculty of Human Sciences, Department of Sport Sciences, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
3 Associate Professor in Exercise Physiology, Faculty of Sport Sciences, Shahrood University of Technology, Shahrood, Iran
Abstract
Objective: The aim of the study was to investigate the effect of two months of endurance training on the expression of Bax and Bcl-2 genes in the brain tissue of Wistar rats with type 2 diabetes.
Methodology: This experimental study included 24 male Wistar rats (mean weight: 200 ± 20 grm, mean age: 8 weeks) randomly assigned to the groups including: healthy control (HC), diabetic control (DC), healthy exercise (HE), and diabetic exercise (DE). The training protocol comprised of running on treadmill that was carried out 5 sessions per week over 8 weeks (30-60 min per session at 18-24 m/ min with 10% imcline). Data were analyzed using one-way ANOVA and Tukey’s post-hoc test at p < 0.05.
Results: The changes in Bax and Bcl-2 expression upon diabetes induction amd exercise training were significantly different among groups (P<0.05). Post-hoc test results indicated a significant difference in Bax gene expression between the diabetic control group and the healthy control group (P = 0.01), the diabetic exercise group (P = 0.008), and the healthy exercise group (P=0.01). Post-hoc test revealed that Bcl-2 gene expression was significantly lower in the diabetic control group compared to the healthy control group (P=0.01) and the healthy exercise group (P=0.003). The Bax to Bcl-2 ratio was significanlty lower in diabetic exercise group compared to the diabetic control (P = 0.01).
Conclusion: A short-term endurance training may partially contribute to improving the apoptotic status of brain tissue in type 2 diabetic rats by modulating Bax and Bcl-2 expression.
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