Exercise Physiology
nooshin salehi aghdam; Roghayeh Pouzesh Jadidi; Karim Azali Alamdari; jabbar bashiri; Mir Ali Reza Nourazar
Abstract
Aim: we investigated the effects of six weeks of HIIT and curcumin supplementation on hepatic cellular death and also GSH level in male rats exposed to drinking water arsenic.Methods: 48 rats were randomized in six groups including HIIT, curcumin, HIIT+curcumin, arsenic, ethanol control and normal control. ...
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Aim: we investigated the effects of six weeks of HIIT and curcumin supplementation on hepatic cellular death and also GSH level in male rats exposed to drinking water arsenic.Methods: 48 rats were randomized in six groups including HIIT, curcumin, HIIT+curcumin, arsenic, ethanol control and normal control. Arsenic 5 mg/bw.day and curcumin 15 mg/bw.day, were consumed orally for entire the study period. HIIT were conducted for six weeks (5 d/w, 60 min/session (with 4 min running at 85-90% of Vo2max and 2 min recovery at 50-60% of Vo2max intervals). Results: Arsenic exposure down regulated hepatic CK18 M65 level and also in a lesser extent; the cellular GSH content. Curcumin supplementation either alone or concomitantly with HIIT, could reverse the changes in hepatic CK18 M65 level. However; only in the HIIT+curcumin group, a remarkable elevation of hepatic GSH level was observed. Conclusion: Arsenic exposure renders liver to an increased oxidative stress and apoptosis level and the both HIIT and curcumin supplementation have appreciable effects to neutralize these hazardous effects. Moreover, only the synergistic effect of HIIT and curcumin supplementation could attenuate the liver antioxidants following arsenic exposure. However, more investigations remains to be done because of the study limitations and lack of similar evidence in this area.
Exercise Physiology
Aghil Mahdavi; Roghayeh Pouzesh Jadidi; Karim Azali Alamdari
Abstract
Introduction: The aim of this study was to evaluate the effects of high-intensity interval training (HIIT) and curcumin supplementation on cardiac heat shock proteins level and gene expression level of microRNAs in rats exposed to arsenic.Methods: 32 male Wistar rats were randomly divided into four groups ...
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Introduction: The aim of this study was to evaluate the effects of high-intensity interval training (HIIT) and curcumin supplementation on cardiac heat shock proteins level and gene expression level of microRNAs in rats exposed to arsenic.Methods: 32 male Wistar rats were randomly divided into four groups of Control, Curcumin, Exercise and concomitant(Curcumin+Exercise). Arsenic 5 mg/kg.day and curcumin 15 mg/kg.day, were consumed orally for entire the study period. HIIT were conducted for six weeks (5 d/w, 60 min/session (consisted of 10 intervals of running (4 min) at 85-90% of Vo2max with 2 min recovery at 50-60% of Vo2max). Tissue protein content and gene expression levels was assessed by Western blotting and PCR methods respectively. Results: No between group difference was observed for gene expression levels of miR-21(P>0.05).The cardiac HSP60, HSP20 levels and the gene expression levels of miR-30 was significantly higher in all three intervention groups compared to Control group(P<0.001). Moreover, the cardiac HSP60, HSP20 content was significantly higher in concomitant group compared to both groups of Exercise and Curcumin(P<0.05). Conclusion: Arsenic exposure may likely increase the vulnerability of cardiomyocytes to injury by altering the amount of heat shock proteins content and the expression levels of microRNAs. Both HIIT and curcumin supplementation could be likely effective to reduce the hazards of arsenic within heart, However, the concomitant intervention might be had more beneficial effects. However, more research is still needed to be done due to the lack of similar evidence and no comprehensive measurement of the influential factors.
Exercise Physiology
Amir Dadashzadeh; Roghayeh Pouzesh Jadidi; Omid Azin Fam; Jabraeel Pouzesh Jadidi
Abstract
Aim: Was to investigate the effects of eight weeks aerobic training and curcumin supplementation on some mitophagy indices induced by ischemia/reperfusion of male rats. Methods: Fifty eight (age: 12 weeks, weight: 315.23 ± 28.57 gr) male rats were randomized into five groups including on Healthy ...
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Aim: Was to investigate the effects of eight weeks aerobic training and curcumin supplementation on some mitophagy indices induced by ischemia/reperfusion of male rats. Methods: Fifty eight (age: 12 weeks, weight: 315.23 ± 28.57 gr) male rats were randomized into five groups including on Healthy control, Ischemic cotrol, Curcumin, Training, Training+Curcumin(Concomitant). Aerobic training program were conducted for eight weeks (5 d/w) starting with running at a speed of 10 m/min, 5% incline for 10 min per day. The running speed and time were gradually increased up to 15-20 m/min per day. In the last two sessions, the intensity of aerobic training reached 25 m/min for 30 min per day with 2 min recovery period at 10 m/min. curcumin (200 mg/bw.day) were consumed through oral gavage for six weeks. The gene expression levels of miR-1 and miR-133 were evaluated using Real-Time PCR method and the data were analyzed using one-way analysis of variance and Tukey post hoc test at the significance level of p <0.05. Results: In all four intervention groups, including ischemia control, exercise, curcumin and combination, the expression of HIF-1α and BNIP3 in renal tissue increased significantly (p=0.001 in all groups) compared to the control group. However, the expression of HIF-1α and BNIP3 genes in renal tissue after exercise caused a significant decrease and increase (p=0.007 and p=0.01, respectively) compared to the healthy control and ischemic control groups. Also, curcumin and Concomitant were associated with an increase in HIF-1α and BNIP3 gene expression in renal tissue
B Ghanivand; B Nakhostinroohi
Abstract
Aim: Curcumin is as an anti-inflammatory and antioxidant agent. The aim of this study was to evaluate the effect of curcumin supplementation on muscle damage indices following intensive eccentric exercise.
Method: Ten healthy, nonsmoking, active young men participated in this cross over study. Participants ...
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Aim: Curcumin is as an anti-inflammatory and antioxidant agent. The aim of this study was to evaluate the effect of curcumin supplementation on muscle damage indices following intensive eccentric exercise.
Method: Ten healthy, nonsmoking, active young men participated in this cross over study. Participants were randomized in a double-blind placebo-controlled fashion in two placebo (P), and curcumin (C) groups. After first blood sampling, subjects did a session of intensive squat exercise. Immediately after exercise, a second blood sampling was collected. Afterward, subjects took 150 mg curcumin or placebo, and sampling was performed 24, 48, and 72h after exercise blood sampling was done again. After a two weeks recovery period and with the change of supplements the test were repeated. Creatine Kinase (CK), and bilirubin were measured as muscle damage markers. Visual Analogue Scale (VAS), Range of Motion of the knee (ROM), and tissue swelling were measured at all time series.
Results: CK significantly decreased in C group 24h after exercise compared with the P group (P<0.05). Bilirubin significantly decreased in C group 72h after exercise compared with the P group (P<0.05). VAS was increased in both in both group exercise, but showed significant decrease in C group compared with P group 48 and 72h after exercise (P<0.05).
Conclusion: It seems 150mg curcumin supplementation after a session of intensive eccentric exercise ameliorate pain and muscle damage through its analgesic, anti-inflammatory, and anti-oxidative properties..
