Fatemeh Kazeminasab
Abstract
Introduction: The aim of this study was to investigate the effect of aerobic exercise on the expression of lncRNAs associated with Glucose 6 phosphatase (G6P) and Glucokinase (GCK) in obese diabetic mice. Materials and methods: Eighteen C57BL/6 mice were divided into two groups: mice fed a high-fat diet ...
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Introduction: The aim of this study was to investigate the effect of aerobic exercise on the expression of lncRNAs associated with Glucose 6 phosphatase (G6P) and Glucokinase (GCK) in obese diabetic mice. Materials and methods: Eighteen C57BL/6 mice were divided into two groups: mice fed a high-fat diet (n=12) and mice fed a standard diet (n=6) for 16 weeks. Then, diabetic mice were divided into two groups: high-fat diet-exercise, high-fat diet-sedentary. The exercised mice were trained on a treadmill for 8 weeks at an average speed of 21 m/min for 50 minutes. Twenty-four hours after the last training session, the mice were sacrificed. Liver tissue was isolated and stored at -80°C. Results: The results showed that levels of hepatic glucose 6 phosphatase and MEG3 increased following a high-fat diet and decreased significantly in trained mice compared to untrained mice. In addition, the expression of glucokinase and lncLGR decreased in diabetic rats, but increased after exercise. Conclusion: The expression of Glucose 6 phosphatase may be regulated in liver tissue probably due to physical exercise and decreased MEG3 lncRNA expression. Exercise reduces the expression of MEG3, which regulates the expression of G6P in liver tissue. Under these conditions, gluconeogenesis and hepatic glucose production was reduced and insulin resistance was improved. Exercise also increased lncLGR expression, possibly leading to increased hepatic GCK expression. In this condition, blood glucose was reduced and stored in the form of glycogen in the liver.
