Exercise Physiology
tahereh shadpour ali zadeh; Mohammad ali Azarbayjani; sirvan atashak; maghsoud peeri; saleh Rahmati ahmad abad
Abstract
AbstractAim:Menopause is associated with an imbalance in the expression of atherogenesis genes in the motor nerve terminal. However, the effect of resistance training on the gene expression affecting the inhibition of sarcopenia in the neurotransmitter plate is not well understood.The purpose of this ...
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AbstractAim:Menopause is associated with an imbalance in the expression of atherogenesis genes in the motor nerve terminal. However, the effect of resistance training on the gene expression affecting the inhibition of sarcopenia in the neurotransmitter plate is not well understood.The purpose of this study was to determine the effect of 8 weeks of increasing resistance training on the gene expression of AKT and mTORC-1 in the motor end plate of the quadriceps muscle of post-menopausal wistar rat.Methods:In this experimental study, 20 female Wistar rats (8 to 12 weeks old) with an average weight of 235 to 265 g, were randomly divided into 2 groups including Resistance Training (RT) and Control (C).Ovariectomy was performed after anesthesia with an intraperitoneal injection of Ketamine/Xylazine mixture with a bilateral incision in the lumbar region. incremental resistance training was performed for 8 weeks, 5 days a week on an 85 degree incline ladder. After 24 hours of the last training session and afterward recovery, animals were sacrificed and their quadriceps muscle was extracted. Real-time PCR was used to determine the expression of AKT and mTORC-1 genes.T-test was used at alpha level of 0.05 to compare the independent groups.Results:Genes expression AKT and Mtorc-1 were increased in the training group compared to the control(P=0.001 and P=0.003 respectively).The rats body weight did not show significant change in any of the groups(P=0.151).Conclusion:It seems that increasing resistance training reduces sarcopenia by increasing the genes expression of AKT and mTORC-1 in the motor end plate.
Exercise Metabolism
ABAZAR Teimoori; Alireza Ruzbahani; ZAhra Karimi mehr
Abstract
Aim:. The purpose of this study was to investigate The effect of eight weeks of increasing resistance training and BCAA nanoliposome supplementation on MiR-200a, HSP60, soleus muscle mitochondrial gene expression and serum IGF-1 in aged male rats.Methods: 32 old male were randomly divided into 4 groups: ...
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Aim:. The purpose of this study was to investigate The effect of eight weeks of increasing resistance training and BCAA nanoliposome supplementation on MiR-200a, HSP60, soleus muscle mitochondrial gene expression and serum IGF-1 in aged male rats.Methods: 32 old male were randomly divided into 4 groups: control, supplement (BCAA), combined (resistant training + BCAA) and exercise. Resistance training consisted of eight weeks of ladder training with moderate intensity (70% of MVCC) and five days a week. Rats in the supplement and combined groups received BCAA nanoliposome supplement at the rate of 600 mg per kilogram of body weight by gavage 5 days a week for 8 weeks. MiR200a and HSP60 were obtained using real-time PCR method and serum IGF-1 was obtained using ELISA method. Statistical analysis was performed with two-way, one-way analysis of variance and Tukey's post hoc test.Finding: significant decrease in MiR-200a and a increase in the expression of the HSP60 gene and serum IGF-1 of rats in the combination and exercise group compared to the supplement and control groups (p=0.001)., No significant difference was observed between the control and supplement groups (p=0.105).Conclusion: The direct effect of miR-200a and HSP60 on IGF-1 signal has an important effect on muscle growth and atrophy. The increase of IGF-1 as a result of strength training and BCAA supplementation causes hypertrophy and creates an anabolic environment and can affect age-related muscle growth factors and can bring benefits in this regard for the elderly.Keywords: Resistance training, BCAA, micro RNA, HSP60.
