Document Type : Research Paper I Open Access I Released under (CC BY-NC) license
Authors
1 Department of Sports Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2 . Department of Sports Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
3 Department of Sports Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz,, Ahvaz,, Iran.
4 Department of Sports Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract
Abstract
Introduction:The purpose of this study was to investigate the effect of eight weeks of high intensity interval training (HIIT) on the levels of SERCA2 and Akt proteins in the heart tissue of rats with type 2 diabetes.
Methodology: In the present study, 40 male rats with an age of 8 weeks and an average weight of 200-220 grams were randomly divided into four groups, each group including 10 rats: 1 - healthy control (saline): 2 - type 2 diabetic control, 3 training groups and 4- the diabetes training group was divided into type 2. After a period of high-fat diet and then induction of diabetes with STZ (a dose of 35mg/kg), exercise program 4-10) two-minute intervals with They did 80-95 maximum speed intensity for 8 weeks and 5 sessions per week. Tissue samples were taken 48 hours after the last training session to check the levels of SERCA2 and Akt proteins using western blot method. One-way ANOVA test and Tukey's post hoc test were used for data analysis.
Findings: The protein expression of SERCA2 and Akt significantly decreased in the heart tissue of diabetic rats compared to healthy rats(P>0.0001). Eight weeks of training significantly increased the protein expression of SERCA2 and Akt in the diabetic and healthy groups compared to the group increased control.
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