نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 استاد دانشگاه گیلان
2 دانشجوی دکتری فیزیولوژی ورزشی
چکیده
هدف: هدف از این مطالعه بررسی اثر فعالیت هوازی با و بدون محدودیت جریان خون (BFR) بر لاکتات، کورتیزول پلاسما و محتوای پروتئین PGC-1α عضله بود.
روش‌شناسی: پنج مرد سالم تمرین نکرده (با میانگین و خطای معیار سن: 02/1±4/33 سال؛ توده بدن: 69/4±64/79 کیلوگرم؛ قد: 02/9±4/173 سانتی متر؛ چربی بدن: 22/2±97/18 درصد) در دو وهله جداگانه مورد مطالعه قرار گرفتند: (1) فعالیت هوازی به همراه BFR شامل راه رفتن بر روی تردمیل با شدت 40% از توان هوازی بیشینه (VO2max) و (2) راه رفتن در شرایط مشابه بدون BFR (به عنوان کنترل). نمونه های بیوپسی عضلانی قبل و 3 ساعت پس از هر وهله فعالیت از عضله پهن جانبی برای تعیین بیان پروتئین PGC-1α گرفته شد، نمونه های خونی نیز قبل از فعالیت، بلافاصله بعد از فعالیت و 2 ساعت بعد فعالیت جهت بررسی تغییرات لاکتات و کورتیزول خون از سیاهرگ بازویی گرفته شد.
یافته‌ها: نتایج نشان داد که محتوای پروتئین PGC-1α سه ساعت پس از یک وهله فعالیت هوازی با BFR به طور معنی‌داری در مقایسه با گروه کنترل افزایش می یابد (05/0>P). لاکتات خون و کورتیزول در هیچ یک از نقطه های زمانی در گروه با و بدون محدودیت جریان خون افزایش معنی‌دار نشان ندادند.
نتیجه‌گیری: نتایج نشان داد که در فعالیت هوازی با BFR، تحریک متابولیکی مکانیسمی برای تنظیم شبکه سیگنالی سلول جهت تحریک بایوژنز میتوکندری نیست. همچنین، بایوژنز میتوکندری یکی از مکانیسم‌های احتمالی افزایش در توان هوازی به همراه تمرین با محدودیت جریان خون است.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of aerobic exercise with and without blood flow restriction on lactate, cortisol and PGC-1α response in human skeletal muscle
نویسندگان [English]
- B Mirzaei 1
- A barjaste 2
- F Rahmani-nia 1
1 Professor in Exercise Physiology, University of Guilan
2 PhD Student in Exercise Physiology, University of Guilan
چکیده [English]
Aim: This study aimed to examine the effect of aerobic exercise with and without BFR on blood lactate, cortisol and PGC-1α response in human skeletal muscle.
Method: On two different occasions, five healthy untrained male subjects (mean±SE; age: 33.4±1.02 years, height: 173.9±4.02 cm, body mass: 79.64±4.69 kg), were required to perform (i) a BFR aerobic exercise at an exercise intensity of 40 % of VO2max; and (ii) similar exercise bouts without BFR (Ctrl). For each condition, baseline and 3 h post-exercise muscle biopsy samples (vastus lateralis) were performed for PGC-1α protein expression analysis. Venous blood samples were also collected at pre-exercise, immediately and 2 h post-exercise to measure changes in blood lactate and serum cortisol.
Results: PGC-1α protein content was significantly higher (P < 0.05) at 3-h post-exercise with BFR compared with Ctrl. Blood lactate and serum cortisol did not significantly change from baseline to immediately after exercise and at 2-h post exercise.
Conclusion: Metabolic stimuli are not a mechanism to mediate cell signaling network responsible for mitochondrial biogenesis. However, the addition of blood flow restriction during aerobic exercise induces an increase in PGC-1α to regulate mitochondrial biogenesis.
کلیدواژه‌ها [English]
- KAATSU
- Vascular Occlusion
- Submaximal Exercise
- Mitochondrial Biogenesis
- Muscle Biopsy
- Blood Lactate
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