نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزشی دانشگاه تبریز، تبریز، ایران
2 دانشیار فیزیولوژی ورزشی دانشگاه تبریز، تبریز، ایران
3 استادیار فیزیولوژی ورزشی دانشگاه تبریز، تبریز، ایران
چکیده
مقدمه: هدف مطالعه حاضر، تعیین تاثیر هشت هفته تمرین تناوبی شدید، تمرین مقاومتی و ترکیبی بر بیان microRNAهای مرتبط با چاقی در پسران نوجوان چاق بود. مواد و روش‌ها: در یک مطالعه نیمه‌تجربی، 36 پسر سالم کم‌تحرک (سن؛ 15-13 سال؛ BMI: 31-28 kg/m2؛ درصد چربی: 36-32 درصد) انتخاب و سپس بر اساس توان هوازی و درصد چربی در چهار گروه همگن تمرین تناوبی شدید، مقاومتی و ترکیبی و کنترل (بدون فعالیت) قرار گرفتند. هر پروتکل تمرینی به مدت چهار جلسه در هفته برای هشت هفته انجام شد. هر جلسه HIIT شامل 8-6 تکرار دویدن 60-30 ثانیه‌ای با 85-80 درصد (سه دقیقه استراحت بین هر تکرار) بود. تمرین مقاومتی شامل حرکات عضلات اصلی بالاتنه، تنه و پایینتنه با شدت 70 تا 90 درصد از 1RM بود. تمرین ترکیبی شامل ترکیب دو جلسه در هفته تمرین مقاومتی و تمرین HIIT در مجموع چهار جلسه تمرین در هفته بود. تغییرات هر یک از شاخص‌‌‌های مورد مطالعه طی مراحل مختلف اندازه‌گیری با آزمون‌های تحلیل واریانس یک‌راهه (ANOVA)، کواریانس و تعقیبی بونفرونی با نرم‌افزار SPSS22 در سطح معنیداری کمتر از 05/0 بررسی شد. نتایج: پس از تائید همگنی گروه‌ها در متغیر پژوهش (05/0<P)، بر اساس نتایج بعد از اتمام هر سه پروتکل تمرینی میزان بیان miRNA-133a، miRNA-1 و miRNA-143 نسبت به مرحله پیش آزمون به طور معنیداری کاهش یافت (05/0>P). همچنین، تنها در گروه ترکیبی تفاوت معنیداری بین تغییرات بیان miRNA-133a و miRNA-1 با گروه تمرین مقاومتی مشاهده شد (05/0>P)، اما میزان بیان miRNA-133a، miRNA-1 و miRNA-143 تفاوتی بین گروه ترکیبی و گروه تمرین HIIT نداشت (05/0<P). نتیجه‌گیری: به نظر می‌رسد استفاده از ترکیب تمرین HIIT و مقاومتی بتواند زمینه کنترل بهتر وزن بدن و چاقی را فراهم کند.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The Effect of Resistance, HIIT and Combined Training on microRNAs Associated with Obesity in Obese and Overweight Adolescent Boys
نویسندگان [English]
- javid nuri 1
- saeid nikokheslat 2
- mostafa khani 3
- javad vakili 2
1 PhD candidate of exercise physiology, University of Tabriz, Tabriz, Iran
2 Associate professor of exercise physiology, University of Tabriz, Tabriz, Iran
3 Assistant professor of exercise physiology, University of Tabriz, Tabriz, Iran
چکیده [English]
Introduction: The aim of the present study is to determine the effect of eight weeks of high intensity interval training, resistance and combined training on hormonal factors and microRNAs related to obesity in obese adolescent boys. Methods: In a semi-experimental study, 36 healthy, sedentary boys (age range: 13-15 years; BMI: 28-31 Kg/m2; Fat Percent: 32-36%) were divided into four homogeneous groups of intense, resistance and combined intermittent exercise and control (no activity) based on aerobic capacity and fat percentage. Each HIIT session consisted of 6-8 repetitions of 30-60 second sprints at 80-85% (three minutes rest between repetitions). Resistance training included movements of the main muscles of the upper body, trunk and lower body. Combined training consisted of a combination of resistance training and HIIT. The changes of the related indicators during different stages of measurement were analyzed with a 3×2 analysis of variance, bonferroni post hoc as appropriate and independent t-tests using SPSS22 software at a significance level of P < 0.05. Results: The results of the present study showed that after completing all three training protocols, the expression levels of miRNA-133a, miRNA-1 and miRNA-143 decreased significantly compared to the pre-test stage. Also, only in the combined group, a significant difference was observed between the expression changes of miRNA-133a and miRNA-1 with the resistance training group, but the expression levels of miRNA-133a, miRNA-1 and miRNA-143 did not differ between the combined group and the HIIT group. Conclusion: It seems that combination of HIIT and resistance training could facilitate body weight and obesity control.
کلیدواژه‌ها [English]
- Obesity
- High-Intensity Interval Training
- Resistance Training
- Combined Training
- Adolescent Boys
- Kansra AR, Lakkunarajah S, Jay MS. Childhood and adolescent obesity: a review. Frontiers in pediatrics. 2021;8:581461.
- Zucker I, Zloof Y, Bardugo A, Tsur AM, Lutski M, Cohen Y, et al. Obesity in late adolescence and incident type 1 diabetes in young adulthood. Diabetologia. 2022;65(9):1473-82.
- B Afshar, F., Nasiri, E., Samadi, A. The Effect of Eight Weeks of Interval Sprint Training on Oxidative Stress Indices of Hippocampus in Male Wistar Rats. Journal Of Metabolism and Exercise, 2022; 12(1): 59-72. doi: 10.22124/JME.2023.23994.267. (in persian)
- Montano M. MicroRNAs: miRRORS of health and disease. Translational Research. 2011;157(4):157-62.
- Ma F, Cao D, Liu Z, Li Y, Ouyang S, Wu J. Identification of novel circulating miRNAs biomarkers for healthy obese and lean children. BMC Endocrine Disorders. 2023;23(1):238.
- Kilic ID, Dodurga Y, Uludag B, Alihanoglu YI, Yildiz BS, Enli Y, et al. MicroRNA-143 and-223 in obesity. Gene. 2015;560(2):140-2.
- Liu J, Wang H, Zeng D, Xiong J, Luo J, Chen X, et al. The novel importance of miR-143 in obesity regulation. International Journal of Obesity. 2023;47(2):100-8.
- Mooren FC, Viereck J, Krüger K, Thum T. Circulating microRNAs as potential biomarkers of aerobic exercise capacity. American Journal of Physiology-Heart and Circulatory Physiology. 2013:ajpheart. 00711.2013.
- Salmasi, M., Tofighi, A., Asri Rezaei, S., Tolouei Azar, J. (Response of Liver Oxidative Indices in Bisphenol A-Infected Rats to Eight Weeks of Aerobic Training with and without Zinc Oxide Supplementation). Journal Of Metabolism and Exercise, 2022; 12(2): -. doi: 10.22124/jme.2023.24007.270. (in persian)
- Bye A, Røsjø H, Aspenes ST, Condorelli G, Omland T, Wisløff U. Circulating microRNAs and aerobic fitness–the HUNT-Study. PloS one. 2013;8(2):e57496.
- McCarthy JJ, Esser KA. MicroRNA-1 and microRNA-133a expression are decreased during skeletal muscle hypertrophy. Journal of applied physiology. 2007;102(1):306-13.
- Care A, Catalucci D, Felicetti F, Bonci D, Addario A, Gallo P, et al. MicroRNA-133 controls cardiac hypertrophy. Nature medicine. 2007;13(5).
- Leuenberger N, Robinson N, Saugy M. Circulating miRNAs: a new generation of anti-doping biomarkers. Analytical and bioanalytical chemistry. 2013;405(30):9617-23.
- Banzet S, Chennaoui M, Girard O, Racinais S, Drogou C, Chalabi H, et al. Changes in circulating microRNAs levels with exercise modality. Journal of applied physiology. 2013;115(9):1237-44.
- Mahdavi, A., Pouzesh Jadidi, R., Azali Alamdari, K. Effects of high-intensity interval training and curcumin supplementation on the amount of heat shock proteins 60 and 20 and the expression of microRNAs 21 and 30 in heart tissue of rats exposed to arsenic. Journal Of Metabolism and Exercise, 2022; 12(1): 35-58. doi: 10.22124/JME.2023.21057.223. (in persian)
- Nielsen S, Scheele C, Yfanti C, Åkerström T, Nielsen AR, Pedersen BK, et al. Muscle specific microRNAs are regulated by endurance exercise in human skeletal muscle. The Journal of physiology. 2010;588(20):4029-37.
- Tonevitsky AG, Maltseva DV, Abbasi A, Samatov TR, Sakharov DA, Shkurnikov MU, et al. Dynamically regulated miRNA-mRNA networks revealed by exercise. BMC physiology. 2013;13(1):9.
- Wahl P, Mathes S, Köhler K, Achtzehn S, Bloch W, Mester J. Acute metabolic, hormonal, and psychological responses to different endurance training protocols. Hormone and Metabolic Research. 2013;45(11):827-33.
- Tanner J, Whitehouse R. Clinical longitudinal standards for height, weight, height velocity, weight velocity, and stages of puberty. Archives of disease in childhood. 1976;51(3):170-9.
- Drummond MJ, McCarthy JJ, Fry CS, Esser KA, Rasmussen BB. Aging differentially affects human skeletal muscle microRNA expression at rest and after an anabolic stimulus of resistance exercise and essential amino acids. American Journal of Physiology-Endocrinology and Metabolism. 2008;295(6):E1333-E40.
- Safdar A, Abadi A, Akhtar M, Hettinga BP, Tarnopolsky MA. miRNA in the regulation of skeletal muscle adaptation to acute endurance exercise in C57Bl/6J male mice. PloS one. 2009;4(5):e5610.
- Habibi P, Alihemmati A, NourAzar A, Yousefi H, Mortazavi S, Ahmadiasl N. Expression of the Mir-133 and Bcl-2 could be affected by swimming training in the heart of ovariectomized rats. Iranian journal of basic medical sciences. 2016;19(4):381.
- Mooren FC, Viereck J, Krüger K, Thum T. Circulating microRNAs as potential biomarkers of aerobic exercise capacity. American Journal of Physiology-Heart and Circulatory Physiology. 2013;306(4):H557-H63.
- Abdellatif M. The role of microRNA-133 in cardiac hypertrophy uncovered. Am Heart Assoc; 2010.
- Simaitis S, Schulte-Körne B, Schiffer T, Bloch W, Predel H-G, Brixius K, et al. Evidence for training-induced changes in miRNA levels in the skeletal muscle of patients with type 2 diabetes mellitus. Frontiers in Physiology. 2020;11.
- Jordan SD, Krüger M, Willmes DM, Redemann N, Wunderlich FT, Brönneke HS, et al. Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism. Nature cell biology. 2011;13(4):434-46.
- Cui SF, Wang C, Yin X, Tian D, Lu QJ, Zhang CY, et al. Similar responses of circulating microRNAs to acute high-intensity interval exercise and vigorous-intensity continuous exercise. Frontiers in physiology. 2016;7:102.
- Aoi W, Ichikawa H, Mune K, Tanimura Y, Mizushima K, Naito Y, et al. Muscle-enriched microRNA miR-486 decreases in circulation in response to exercise in young men. Frontiers in physiology. 2013;4:80.
- Baggish AL, Hale A, Weiner RB, Lewis GD, Systrom D, Wang F, et al. Dynamic regulation of circulating microRNA during acute exhaustive exercise and sustained aerobic exercise training. The Journal of physiology. 2011;589(16):3983-94.
- Wahl P, Wehmeier UF, Jansen FJ, Kilian Y, Bloch W, Werner N, et al. Acute Effects of Different Exercise Protocols on the Circulating Vascular microRNAs-16,-21, and-126 in Trained Subjects. Frontiers in Physiology. 2016;7.
- Nielsen S, Åkerström T, Rinnov A, Yfanti C, Scheele C, Pedersen BK, et al. The miRNA plasma signature in response to acute aerobic exercise and endurance training. PloS one. 2014;9(2):e87308.
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