نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، تهران، ایران
2 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشگاه پیام نور، تهران، ایران
چکیده
مقدمه: با توجه به ویژگی‌های حفاظتی و آنتی اکسیدانی فعالیت ورزشی و اثرات متناقض آن بر متغیرهای وابسته مورد مطالعه در بیمارانی که از دیابت و مصرف داروهای شیمیایی به مدت طولانی رنج می‌برند،هدف از این مطالعه تأثیر یک دوره تمرین هوازی با شدت زیاد و مصرف مکمل کوئرستین بر سطوح آدیپونکتین و فاکتور رشد اندوتلیال عروقی در بافت قلب رت‌های مبتلا به دیابت بود. مواد و روشها: بدین منظور50 سر رت نر ویستار با میانگین وزنی33±237گرم بطور تصادفی در پنج گروه 10 تایی شامل:کنترل سالم، دیابت، دیابت+مکمل، دیابت+تمرین و دیابت+مکمل+تمرین قرار گرفتند. پروتکل تمرینی شامل هشت هفته تمرین هوازی با شدت متوسط، پنج جلسه در هفته و 60 دقیقه در هر جلسه بود و گروه‌های مکمل نیز بصورت گاواژ روزانه به‌مقدار 50 میلی‌گرم به ازای کیلوگرم وزن بدن کوئرستین دریافت کردند. تغییرات سطوح آدیپونکتین و فاکتور رشد اندوتلیال عروقی با استفاده از روش الایزا مورد بررسی قرار گرفت. برای تجزیه و تحلیل داده‌ها از آنالیز واریانس یک‌طرفه و آزمون توکی استفاده شد. یافته‌ها: نتایج نشان داد که اختلاف معنی داری بین گروه‌های پژوهش در مقادیر آدیپونکتین و فاکتور رشد اندوتلیال عروقی وجود دارد (001/0P=). تغییرات آدیپونکتین و فاکتور رشد اندوتلیال عروقی بین گروه‌های تمرین+مکمل،تمرین و مکمل با دیابت نیز تفاوت معنی‌داری داشت (001/0P=). نتیجه‌گیری: به نظر می‌رسد که استفاده از مکمل کوئرستین و تمرینات تداومی شدید منجر به افزایش سطوح آدیپونکتین و VEGF در موش‌های دیابتی شده است و به طور کلی بیان کننده اهمیت آنها در کاهش عوامل خطرساز در بیماری دیابت است.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The effect of aerobic exercise and quercetin supplementation on the levels of adiponectin and vascular endothelial growth factor in the heart tissue of rats with type 2 diabetes
نویسندگان [English]
- Seyyede Neda Delfani Hossein 1
- Ali Barzegari 2
- Saeid Naghibi 2
- mohamad hassan dashty 2
1 Department of Physical Education and Sports Science, Payame Noor UniversityTehran, Iran
2 Assistant Professor, Department of Physical Education and Sports Science, Payame Noor UniversityTehran, Iran
چکیده [English]
Introduction: Considering the protective and antioxidant properties of exercise training and its contradictory effects on the dependent variables studied in patients suffering from diabetes and taking chemical drugs for a long time, the aim of this study was the effect of a period of high-intensity aerobic exercise and quercetin supplementation on the levels of adiponectin and vascular endothelial growth factor in the heart tissue of diabetic rats. Methods: 50 male Wistar rats with an average weight of 237±33 grams were randomly divided into five groups of 10 including: healthy control, diabetes, diabetes+supplement, diabetes+exercise and diabetes+supplement+exercise. The protocol included eight weeks of moderate intensity aerobic exercise, five sessions per week and 60 minutes per session, and the supplement groups also received quercetin in the amount of 50 mg per kg of body weight by daily gavage. Changes in the levels of adiponectin and vascular endothelial growth factor were investigated using the ELISA. One-way analysis of variance and Tukey's test were used to analyze the data.Findings: The results showed that there is a significant difference between groups in the values of adiponectin and vascular endothelial growth factor (P=0.001). The changes of adiponectin and vascular endothelial growth factor between exercise+supplement, exercise and supplement with diabetes groups were also significantly different (P=0.001).Conclusion: It seems that the use of quercetin supplement and intense continuous training has led to an increase in the levels of adiponectin and VEGF in diabetic rats, and it indicates their importance in reducing risk factors in diabetes.
کلیدواژه‌ها [English]
- moderate intensity aerobic exercise
- diabetes
- adiponectin
- vascular endothelial growth factor
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