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لیست داوران سال 1401

لیست داوران سال 1400

اثر تمرین هوازی همراه با رزوراترول بر شاخص‌های التهابی و آنتی‌اکسیدانی هیپوکمپ موش‌های مبتلا به آلزایمر

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I

نویسندگان

1 گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

2 دانشیار گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

3 گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی ، آمل

چکیده

مقدمه: تا به امروز هیچ مراقبت یا درمان موثری برای بیماری آلزایمر (AD) وجود ندارد. افزایش فعالیت ورزشی منظم و رژیم غذایی حاوی پلی‌فنل‌ها، رویکردهای غیردارویی موثری هستند که از پیشرفت بیماری‌های عصبی جلوگیری می‌کنند. در این مطالعه، نشانگرهای التهاب و آنتی‌اکسیدانی پس از هشت هفته تمرین ورزشی (T)، درمان با رزوراترول (RSV) یا درمان ترکیبی در مدل موش AD مورد بررسی قرار گرفت. روش‌ها: در این مطالعه تجربی، 34 سر موش صحرایی نر نژاد ویستار (وزن 07/9±17/223 گرم و سن هشت هفته) در پنج گروه نرمال (NO)، آلزایمر (AD)، آلزایمر-تمرین (ADT)، آلزایمر-رزوراترول (ADRSV) و آلزایمر-تمرین-رزوراترول (ADTRSV) قرار گرفتند. القای آلزایمر با استفاده از دو میکرولیتر آمیلوئید بتا انجام شد. گروه­های مکمل، طی دوره مداخله روزانه 20 میلی‌گرم RSV (به ازای هر کیلوگرم وزن بدن) را به صورت خوراکی دریافت کردند. برنامه تمرین هوازی شامل دویدن روی تردمیل با سرعت 18-6 متر در دقیقه، پنج روز هفته به مدت هشت هفته اجرا شد. شاخص‌های آنتی‌اکسیدانی به روش فلوریمتری و اسپکتوفتومتری و بیان ژن‌ها به روش RT-PCR اندازه‌گیری شد. یافته‌ها: القای AD باعث کاهش معنی‌داری در شاخص‌های آنتی اکسیدانی (GPx، SOD و CAT) و افزایش MDA، NF-κB، TNF-α و IL-1β  شد (0001/0=p). تمرین و RSV شاخص‌های آنتی‌اکسیدانی را افزایش داده و میزان بیان عوامل التهابی را کاهش داد (p<0.05). همچنین افزایش معنی‌داری در میزان GPx و SOD در گروه ADTRSV نسبت به گروه‌ ADT (به ترتیب 043/0p=، 041/0p=) و ADRSV (به ترتیب 026/0p=، 035/0p=) مشاهده شد. بیان  NF-κB و TNF-α در گروه ADTRSV نسبت به گروه‌ ADT (به ترتیب 040/0p= و 041/0p=) و ADRSV (044/0p= و 034/0p=) کاهش معنی‌داری داشت. نتیجه گیری: فعالیت ورزشی هوازی و رزوراترول می‌تواند استراتژی درمانی خوب برای جلوگیری از پیشرفت بیماری AD از طریق کاهش التهاب و فشار اکسایشی باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of aerobic training with resveratrol on the inflammatory and antioxidant indices of the hippocampus of rats with Alzheimer's disease

نویسندگان [English]

  • Ammar Rashet 1
  • Ahmad Abdi 2
  • alireza barari 3

1 Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

2 Associate Professor, Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.

3 department of sport physiology,ayatollah amoli branch,,islamic azad university

چکیده [English]

Introduction: To date, there is no cure or effective treatment for Alzheimer’s disease (AD). Promoting regular exercise and a diet containing polyphenols are effective non-pharmacological approaches that prevent the progression of neurodegenerative diseases. In this study, inflammation and antioxidant markers were examined after eight weeks of exercise training (T), treatment with resveratrol (RSV), or a combination treatment in an Alzheimer's disease rat model. Materials & Methods: In this experimental study, 35 male Wistar rats (weight 223.17±9.07 grams and eight weeks old) were divided into 5 groups: Normal (NO), Alzheimer's (AD), Alzheimer's-Training (ADT), Alzheimer's-Resveratrol (ADRSV) and Alzheimer's-Training-Resveratrol (ADTRSV). Alzheimer's was induced using two microliters of amyloid-beta. The supplement groups received 20 mg of RSV (per kg of body weight) orally during the intervention period. Aerobic exercise program including running on treadmill with a speed of 6-18 meters per minute, was performed 5 days a week for eight weeks. Antioxidant indices were measured using fluorimetry and spectrophotometry, while gene expression was assessed by the RT-PCR method. Results: AD induction caused a significant decrease in antioxidant indices (GPx, SOD and CAT) and increased MDA, NF-κB, TNF-α and IL-1β (p=0.0001). Exercise and RSV decreased and increased the antioxidant indices and the expression of inflammatory factors (p<0.05). It was also observed a significant increase in Gpx and SOD levels in the ADTRSV compared to the ADT (p=0.043, p=0.041) and ADRSV (p=0.026, p=0.035, respectively). The expression of NF-κB and TNF-α was significantly decreased in ADTRSV compared to ADT (p=0.040 and p=0.041) and ADRSV (p=0.044 and p=0.034). Conclusion: Aerobic training and resveratrol may serve as an effective therapeutic strategy to prevent the progression of Alzheimer's disease by reducing inflammation and oxidative stress.

کلیدواژه‌ها [English]

  • Exercise
  • Resveratrol
  • Neuroinflammation
  • Antioxidants
  • Alzheimer's disease
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سوخت و ساز و فعالیت ورزشی
دوره 14، شماره 2 - شماره پیاپی 2
آبان 1403
صفحه 105-125
فایل ها
سابقه مقاله
  • تاریخ دریافت: 30 آذر 1402
  • تاریخ بازنگری: 14 شهریور 1403
  • تاریخ پذیرش: 25 آبان 1403
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