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لیست داوران سال 1401

لیست داوران سال 1400

تاثیر هشت هفته تمرین هوازی تناوبی با شدت بالا بر سطوح پروتئین ها SERCA2 و Akt کاردیومیوسیت موش های صحرایی مبتلا به دیابت نوع 2

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I

نویسندگان

1 دانشیار گروه فیزیولوژ ی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران.

2 استادیار گروه فیزیولوژ ی ورزشی، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران.

چکیده

مقدمه: هدف ‌از پژوهش حاضر بررسی تأثیر هشت هفته تمرین‌ تناوبی با شدت بالا (HIIT) بر سطوح پروتئین‌هایSERCA2a   و Akt بافت قلب موش‌های صحرایی مبتلا به دیابت نوع دو بود. روش شناسی: در مطالعه حاضر 40 سر موش صحرایی نر با سن 8 هفته و میانگین وزن 220-200گرم، به چهار گروه 1-کنترل سالم؛ 2-کنترل دیابت؛ 3-گروه سالم تمرین و 4-گروه تمرین دیابت تقسیم شدند. پس از هشت هفته رژیم غذایی پر چرب و سپس القاء دیابت با STZ (یک دوزmg/kg ۳۵)، برنامه تمرین را به مدت ۸هفته و 5جلسه در هفته انجام دادند. 48ساعت بعد از آخرین جلسه تمرین، بافت قلب استخراج و بررسی سطوح پروتئین‌ها با استفاده از روش وسترن‌بلات انجام گرفت. برای تحلیل داده­ها پس از انجام آزمون آنوای دو‌راهه برای بررسی اثر تعاملی دیابت و تمرین، از آزمون آنوای یک‌طرفه و همچنین آزمون تعقیبی توکی برای بررسی تغییرات بین گروه‌ها استفاده شد. سطح معناداری 05/0>P در نظر گرفته شد. از نرم افزار SPSS نسخه 25 برای تجزیه و تحلیل داده­ها استفاده شد. یافته­ها: بیان پروتئین SERCA2a و Akt به طور معناداری در بافت قلب موش‌های دیابتی در مقایسه با موش­های سالم کمتر شد(P<0.0001). هشت هفته تمرین بیان پروتئین SERCA2a و Akt را در گروه‌های دیابتی و سالم به طور معناداری نسبت به گروه‌های کنترل بیشتر کرد. نتیجه‌گیری: نتایج این پژوهش به طور کلی نشان داد که تمرینات HIIT احتمالاً می­تواند تغییرات نامطلوب ناشی از دیابت را در بیان این پروتئین‌ها تعدیل کند و به عنوان یک راهبرد غیردارویی امیدوارکننده باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of eight weeks of high-intensity intermittent aerobic training on the levels of SERCA2 and Akt proteins Cardiomyocytes of rats with type 2 diabetes

نویسندگان [English]

  • Ali Shabani Fard 1
  • Mohsen Ghanbarzadeh 1
  • Aliakbar Alizadeh 2
  • Rouhollah Ranjbar 1

1 Department of Sports Physiology, Faculty of Sports Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 Assistant Professor, Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

چکیده [English]

Introduction: The purpose of this study was to investigate the effect of eight weeks of high intensity interval training (HIIT) on the levels of SERCA2a and Akt proteins in the heart tissue of rats with type 2 diabetes. Methodology: In the present study, 40 male rats with an age of 8 weeks and an average weight of 200-220 grams were divided into four groups: 1- healthy control (saline); 2- diabetes control; 3- Healthy exercise group and 4- Diabetic exercise group. After eight weeks of high-fat diet and then induction of diabetes with STZ (one dose of 35 mg/kg), they did the exercise program for 8 weeks and 5 sessions per week. 48 hours after the last training session, heart tissue was extracted and protein levels were checked using western blot method. To analyze the data, after performing the two-way ANOVA test to investigate the interactive effect of diabetes and exercise, one-way ANOVA test was used to investigate the changes between groups. A significance level of P<0.05 was considered. SPSS version 25 software was used for data analysis. Results: The protein expression of SERCA2a and Akt decreased significantly in the heart tissue of diabetic rats compared to healthy rats (P<0.0001). Eight weeks of training significantly increased SERCA2a and Akt protein expression in diabetic and healthy groups compared to control groups. Conclusion: The results showed that HIIT exercises can possibly moderate the adverse changes caused by diabetes in the expression of these proteins and is promising as a non-pharmacological strategy.

کلیدواژه‌ها [English]

  • Type2 Diabetes
  • Heart
  • SERCA2
  • Akt
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سوخت و ساز و فعالیت ورزشی
دوره 14، شماره 2 - شماره پیاپی 2
آبان 1403
صفحه 125-144
فایل ها
سابقه مقاله
  • تاریخ دریافت: 29 اسفند 1402
  • تاریخ بازنگری: 13 تیر 1403
  • تاریخ پذیرش: 14 تیر 1403
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