نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 پژوهشگر پسادکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه کردستان، سنندج، ایران.
2 گروه فیزیولوژی ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه کردستان، سنندج، ایران.
3 گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، گروه فیزیولوژی ورزشی، تهران، ایران.
4 گروه فیزیولوژی و فارماکولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی کردستان، سنندج، ایران.
5 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران.
چکیده
مقدمه: دیابت نوع 2 (T2DM) یک اختلال متابولیک مزمن است که با مقاومت به انسولین و هایپرگلیسمی مشخص میشود و اغلب با اختلال در تنظیم متابولیسم لیپید و افزایش فعالیت اتوفاژی همراه است. این مطالعه به بررسی تأثیر تمرین تناوبی شدید (HIIT) و تمرین تداومی متوسط (MICT) بر سطوح پروتئینهای اتوفاژی (Beclin-1، LC3-II و PLIN-2) در بافت چربی احشایی رتهای دیابتی پرداخت.
روش کار: رتهای نر ویستار به گروههای کنترل سالم، کنترل دیابتی، دیابتی تحت تمرین HIIT و دیابتی تحت تمرین MICT تقسیم شدند. دیابت با تزریق استرپتوزوتوسین و رژیم پرچرب القا شد. برنامه تمرینی به مدت ۸ هفته اجرا گردید. سطوح پروتئینها با وسترن بلات و شاخصهای متابولیک با روشهای استاندارد اندازهگیری شدند.
یافتهها: دیابت موجب افزایش معنادار Beclin-1، LC3-II و 2 PLIN- شد. هر دو تمرین HIIT و MICT این سطوح را کاهش دادند، اما MICT تأثیر قویتری بر کاهش Beclin-1 و LC3-II داشت. همچنین، هر دو تمرین باعث کاهش گلوکز خون، انسولین و مقاومت به انسولین شدند.
نتیجهگیری: به نظر میرسد تمرینات ورزشی، بهویژه MICT، میتوانند با تنظیم منفی پروتئینهای اتوفاژی، نقش محافظتی در برابر اختلالات ناشی از دیابت نوع ۲ ایفا کنند. این یافتهها بر اهمیت تمرینات ورزشی در کنترل دیابت تأکید دارند.
کلیدواژهها
موضوعات
عنوان مقاله [English]
Investigation of Excessive Autophagy in Visceral Adipose Tissue of Type 2 Diabetic Rats Following Eight Weeks of High-Intensity Interval Training and Moderate-Intensity Continuous Training
نویسندگان [English]
- Hadi Golpasandi 1
- Mohammad Rahman Rahimi 2
- Peyman Ghasemi 3
- Slahadin Ahmadi 4
- Siamand Abdollahpour 5
1 Postdoctoral Researcher in exercise Physiology, Department of exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran.
2 Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran.
3 Department of Exercise Physiology, Faculty of Humanities, Department of Exercise Physiology, Tehran, Iran.
4 Department of Physiology and Pharmacology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
5 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran.
چکیده [English]
Introduction: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance and hyperglycemia, often accompanied by dysregulation of lipid metabolism and enhanced autophagic activity. This study examined the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on autophagy-related proteins (Beclin-1, LC3-II, and PLIN-2) in visceral adipose tissue of diabetic rats.
Methodology: Male Wistar rats were divided into normal control (NC), diabetic control (DC), diabetic+HIIT (D+HIIT), and diabetic+MICT (D+MICT) groups. Diabetes was induced through streptozotocin injection and high-fat diet. The exercise program was implemented over a period of 8 weeks . Protein levels were measured by Western blot, and metabolic indices were assessed using standard methods.
Results: Diabetes significantly increased Beclin-1, LC3-II, and PLIN-2 levels. Both HIIT and MICT reduced these levels, with MICT showing stronger effects on Beclin-1 and LC3-II reduction. Both exercise regimens also decreased blood glucose, insulin levels, and insulin resistance.
ConclusionsExercise training, particularly MICT, appears to play a protective role against type 2 diabetes complications by downregulating autophagy proteins. These findings highlight the importance of exercise interventions in diabetes management.
کلیدواژهها [English]
- Autophagy
- LC3-II
- HIIT
- MICT
- Diabetic cardiomyopathy
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