نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 دانشجوی دکتری، گروه تخصصی تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی،تهران، ایران.
2 استادیار، گروه تخصصی تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات ، دانشگاه آزاد اسلامی ، تهران، ایران.
3 دانشیار، گروه تخصصی تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
چکیده
مقدمه و هدف: درمان هدفمند عوامل رشد و ایمنی ممکن است منجر به مهار موثرتری از تکثیر و پیشرفت تومور در سرطان ملانوما شود. هدف این پژوهش مطالعه تاثیر شش هفته تمرین هوازی و مصرف عصاره آناناس بر بیان ژن پروتئین شوک گرمایی 70 و القاءکننده متالوپروتئیناز ماتریکس خارج سلولی (CD147) بافت تومور در موشهای مبتلا به سرطان ملانوما بود.
روش کار: این مطالعۀ بنیادی- تجربی بر روی 20 سر موشهای نر نژاد C57BL/6 مبتلا به سرطان ملانوما در چهار گروه شامل کنترل، تمرین هوازی، عصارۀ آناناس و تمرین هوازی- آناناس انجام شد. پس از القای تومور، برنامۀ تمرین را بهمدت شش هفته انجام دادند و عصارۀ آناناس به میزان 300mg/kg گاواژ شد. وزن و حجم تومور موشها و بیان ژن HSP70 و CD147 بافت تومور به روش RT-PCR اندازهگیری شد و دادهها با استفاده آنالیز واریانس یکطرفه، آزمون تحلیل واریانس دو عاملی و تعقیبی بنفرونی مورد تجزیهوتحلیل قرار گرفتند. سطح معنیداری نیز 0/05 ≥P در نظر گرفته شد.
یافتهها: براساس نتایج در گروه تمرین هوازی و تمرین- آناناس کاهش معنیدار حجم تومور و کاهش بیان ژن HSP70 و CD147 بافت تومور مشاهده شد.
نتیجهگیری: تمرینات هوازی بـا شدت متوسط بههمراه عصاره آناناس، با وجـود تـأثیرات مثبت همچون کاهش وزن بدن و حجم تومور، با کاهش بیان ژن HSP70 و CD147 بافت تومور احتمالا منجر به کند شدن روند پیشرفت بیماری ملانوما میگردد و به تنظیم عوامل رشد و ایمنی در سرطان ملانوما کمک میکند.
کلیدواژهها
موضوعات
عنوان مقاله [English]
The effect of six weeks of aerobic training and consumption of pineapple extract on HSP70 and CD147 gene expression in tumor tissues of mice with melanoma cancer
نویسندگان [English]
- Maryam Movassagh 1
- Hossein Abednatanzi 2
- mandana gholami 3
- Farshad Ghazalian 3
1 Department of physical education and sport sciences,SR.C., Islamic Azad university, Tehran, Iran.
2 Department of physical education and sport sciences,SR.C., Islamic Azad university, Tehran, Iran.
3 Department of physical education and sport sciences,SR.C., Islamic Azad university, Tehran, Iran. Tehran, Iran.
چکیده [English]
Introduction and Objective: Targeted therapy of growth and immune factors may lead to more effective inhibition of tumor proliferation and progression in melanoma cancer. The aim of this study was to study the effect of six weeks of aerobic exercise and pineapple extract consumption on the expression of heat shock protein 70 and extracellular matrix metalloproteinase inducer (CD147) in tumor tissue in mice with melanoma cancer.
Methods: This basic-laboratory study was conducted on 20 male C57BL/6 mice with melanoma cancer in four groups including control, aerobic exercise, pineapple extract, and aerobic exercise-pineapple. After tumor induction, the exercise program was performed for six weeks and pineapple extract was gavage at a dose of 300 mg/kg. The weight and volume of the mice's tumors and the expression of HSP70 and CD147 genes in tumor tissue were measured by RT-PCR, and the data were analyzed using one-way analysis of variance, two-way analysis of variance, and post hoc analysis. The significance level was considered to be P≤0.05.
Findings: Based on the results, a significant decrease in tumor volume and a decrease in HSP70 and CD147 gene expression in tumor tissue were observed in the aerobic exercise and pineapple exercise groups.
Conclusion: Moderate-intensity aerobic exercise with pineapple extract, with positive effects such as a decrease in body weight and tumor volume, by reducing HSP70 and CD147 gene expression in tumor tissue, probably leads to a slowdown in the progression of melanoma and helps regulate growth and immune factors in melanoma.
کلیدواژهها [English]
- Key words: aerobic exercise
- pineapple
- melanoma cancer
- HSP70
- CD147
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