نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 گروه فیزیولوژی ورزشی واحد آیت الله آملی، دانشگاه آزاد اسلامی ، آمل، ایران
2 گروه فیزیولوژی ورزشی واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل ، ایران
چکیده
هدف: بیماری آلزایمر یک اختلال عصبی پیشرونده است که با تجمعات پروتئینی، فرایندهای التهابی و اکسیداتیو نابجا به تدریج تواناییهای ذهنی را تحت تاثیر قرار می دهد. تاثیر توام فعالیت هوازی و عصاره رودیولا بر این بیماری به طور کامل شناخته نشد. هدف از پژوهش حاضر اثر همافزای تمرین هوازی و عصاره رودیولا بر BDNF و شاخصهای استرس اکسیداتیو در بافت مغزی موشهای آلزایمری بود.
روش شناسی: در این پژوهش ، تعداد 30 سر موش 6 C57BL/ با میانگین وزن 2±14 گرم و سن 12 هفته به صورت تصادفی به 5 گروه 1)کنترل، سالم 2)آلزایمر، 3)آلزایمر + تمرین، 4)آلزایمر + رودیولا، 5)آلزایمر + تمرین + رودیولا تقسیم شدند. ایجاد مدل آلزایمری از روش تزریق Aβ1-42 درون هیپوکامپ استفاده شد. موشهای گروههای تمرینی به مدت هشت هفته، پنج روز در هفته، هر جلسه 30 تا 45 دقیقه و با سرعت 10 تا 18 متر بر دقیقه روی نوار گردان دویدند. عصاره رودیولا به مقدارmg/kg200 به صورت گاواژ در روزهای تمرین دریافت شد. دادهها از آزمون تحلیل واریانس یک طرفه و آزمون توکی در نرم افزار SPSS تجزیه و تحلیل شدند.
یافتهها: نتایج نشان دادکه بیان ژنBDNF و میزان فعالیت SOD در گروههای آلزایمر+تمرین، آلزایمر+رودیولا، آلزایمر+تمرین+رودیولا در مقایسه با گروه آلزایمری به طور معنیداری افزایش یافت(P<0/001). سطح سرمی MDA در گروههای آلزایمر+تمرین، آلزایمر+رودیولا، آلزایمر+تمرین+رودیولا در مقایسه با گروه آلزایمری به طور معنیداری کاهش یافت(P<0/001).
نتیجه گیری: استفاده همزمان از تمرین هوازی و عصاره Rhodiola rosea، راهبردی مؤثر، غیرتهاجمی و کمعارضه برای تعدیل استرس اکسیداتیو و تقویت بازسازی نورونی در شرایط نورودژنراتیو است.
کلیدواژهها
موضوعات
عنوان مقاله [English]
Synergistic Effects of Aerobic Exercise and Rhodiola Extract on BDNF and Oxidative Stress Markers in the Brain Tissue of Alzheimer’s Rats
نویسندگان [English]
- yosefali salehi firozkalaei 1
- Asieh aAbbassi-Daloii 1
- alireza barari 2
1 Department of Exercise Physiology, Am.C., Islamic Azad University, Amol, Iran.
2 Department of Exercise Physiology, Am.C., Islamic Azad University, Amol, Iran.
چکیده [English]
Objective: Alzheimer is a progressive neurodegenerative disorder that gradually impairs mental abilities through abnormal protein accumulation, as well as inflammatory and oxidative processes.. the present study aimed to investigate the synergistic effects of aerobic exercise and Rhodiola rosea extract on brain-derived neurotrophic factor (BDNF) expression and oxidative stress indices in the brain tissue of mice with Alzheimer..
Methodology: 30 male C57BL/6 mice with an weight of 14 ± 2 g and an age of 12 weeks were randomly assigned to five groups: (1) healthy control, (2) Alzheimer’s, (3) Alzheimer’s + exercise, (4) Alzheimer’s + Rhodiola, and (5) Alzheimer’s + exercise + Rhodiola. The Alzheimer’s model was induced by intrahippocampal injection of Aβ₁₋₄₂. Mice in the training groups performed treadmill running for 30–45 minutes per session, five days per week, at a speed of 10–18 m/min for eight weeks. Rhodiola extract was administered by gavage at a dose of 200 mg/kg on training days. Data were analyzed using one-way ANOVA followed by Tukey test in SPSS.
Results: BDNF gene expression and SOD activity levels in the Alzheimer’s + exercise, Alzheimer’s + Rhodiola, and Alzheimer’s + exercise + Rhodiola groups were significantly higher than those in the Alzheimer’s group (P < 0.001). serum MDA levels were significantly lower in these three intervention groups compared with the Alzheimer’s group (P < 0.001).
Conclusion: the concurrent application of aerobic exercise and Rhodiola rosea extract represents an effective, non-invasive, and low-risk strategy for modulating oxidative stress and promoting neuronal regeneration under neurodegenerative conditions.
کلیدواژهها [English]
- Alzheimer’
- aerobic exercise
- Rhodiola rosea
- BDNF
- SOD
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