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لیست داوران سال 1401

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بررسی اثر شدت های مختلف تمرین استقامتی بر بیان پروتئین اینفلامازوم NLRP-3 بافت چربی احشایی، سطوح سرمی گلوکز و انسولین موش های صحرایی نر دیابتی شده با استرپتوزوسین

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی

2 دانشیار دانشگاه شهرکرد

3 استادیار دانشگاه علوم پزشکی شهرکرد

4 استادیار دانشگاه شهرکرد

چکیده

هدف: فعال سازی اینفلامازوم NLRP-3 از مشخصه های التهاب مزمن در دیابت نوع-2 است که موجب رهایش IL-1β از سلول های ایمنی بافت چربی می شود. با توجه به اینکه به تازگی به نقش اینفلامازوم NLRP-3 در پاتوژنز دیابت نوع 2 توجه زیادی شده است، تاثیر احتمالی تمرینات ورزشی بر این عوامل مورد بررسی قرار نگرفته است.
روش شناسی: 40 سر موش صحرایی دیابتی شده با تزریق STZ به پنج گروه هشت تایی دیابت و تمرین استقامتی کم شدت (DL)، دیابت و تمرین شدت متوسط (DM)، دیابت و تمرین با شدت زیاد (DH)، گروه کنترل دیابتی (D) و کنترل سالم (CON) تقسیم شدند. پروتکل تمرین استقامتی به مدت هشت هفته، چهار جلسه در هفته انجام شد. از روش وسترن بلات برای اندازه گیری بیان پروتئین اینفلامازوم NLRP-3 بافت چربی احشایی و از روش الایزا برای اندازه گیری سطوح سرمی گلوکز و انسولین استفاده شد.
یافته‌ها: نتایج نشان داد کاهش معنی داری در  بیان پروتئین NLRP-3 در  گروه های تمرین استقامتی DM  و DH  در مقایسه با  گروه تمرین استقامتی DL مشاهده شد (05/0P<). بیان کاسپاز-1 به طور معنی داری در گروه­های شدت پایین،  شدت متوسط و شدت بالا در مقایسه با گروه کنترل دیابتی پایین­تر بود (05/0P<). با این وجود، تفاوت معنی داری بین گروه­های تمرینی مشاهده نشد. سطوح انسولین سرمی در گروه تمرین استقامتی DH به طور معنی داری پایین تر از گروه تمرین استقامتی DL بود (05/0P<). در مقایسه با گروه دیابتی، تمرین استقامتی DL، DM  و DH  کاهش معنی داری در سطوح سرمی گلوکز مشاهده شد (05/0P<).  
نتیجه‌گیری: به نظر می رسد تمرین استقامتی با شدت بالا و متوسط موجب تعدیل مثبت بیان اینفلامازوم NLRP3 و بهبود سطوح عوامل گلایسمیک در افراد دیابتی شود.
 

کلیدواژه‌ها

عنوان مقاله [English]

The effect of different intensities of endurance training on NLRP-3 Inflammasome protein expression in visceral adipose tissue, serum glucose levels and insulin in Streptozotocin-induced diabetic rats

نویسندگان [English]

  • A Abbasi 1
  • M Faramarzi 2
  • M Ghatreh Samani 3
  • E Bbanitalebi 4

1 PhD student in Exercise Physiology

2 Associate Professor, Shahrekord University

3 Assistant professor, Shahrekord University of Medical Science

4 Assistant professor, Shahrekord University

چکیده [English]

Aim: NLRP-3 Inflammasome is considered an indicator of chronic diseases such as obesity and type 2 diabetes mellitus (T2DM) which can result in secretion of IL-1β from adipose tissue. Despite numerous investigations on the underlying activation mechanisms of NLRP-3 in pathogenesis of T2DM, less attention has been paid to the impact of exercise on NLRP-3.
Method: Male stereptpzosin-induces diabetic rats (n=40) were randomly assigned to five groups (n=8): diabetic+ low intensity  endurance training (DL), diabetic+ moderate intensity endurance training (DM), diabetic+ high intensity endurance training (DH), non-diabetic with no training(CON), and diabetic with no training(D). Eight weeks of running (four sessions per week) with different intensities were completed. Western blotting method was used to measure NLRP3.  ELISA technique was used to assess serum levels of insulin and glucose.
Results: Results showed that expression of NLRP-3 protein were significantly lower in endurance DM (P<0.05) and endurance DH (P<0.05) groups against DL group. Caspase-1 expression was significantly lower in DL (P=0.001), DM (P<0.05), and DH (P<0.05) compared with D group. However, no significant different was found between the training groups. Serum insulin level was significantly lower in endurance DH group compared with endurance DL group (P<0.05).In comparison with D group, significant reduction of serum glucose was observed in endurance DL group (P<0.05), endurance DM (P<0.05), and endurance DH (P<0.05).
Conclusion: It appears that training with high and moderate intensities induced positive modulation in NLRP-3 expression as well as improved glycemic factors levels.
 

کلیدواژه‌ها [English]

  • Keywords: Training intensity
  • Type 2 diabetes
  • Insulin resistance
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سوخت و ساز و فعالیت ورزشی
دوره 7، شماره 1
اردیبهشت 1396
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  • تاریخ دریافت: 18 مهر 1396
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  • تاریخ پذیرش: 23 دی 1396
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