نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 دانشجوی دکتری بیوشیمی و متابولیسم ورزشی
2 دانشیار دانشگاه مازندران
3 استادیار دانشگاه مازندران
چکیده
هدف: انرژی مازاد بدن به صورت تریآسیلگلیسرول (TAG) در بافت چربی اندامهای مختلف ذخیره میشود. به منظور کاهش ذخایر چربی، باید استفاده از آنها برای تولید انرژی افزایش یابد. هدف پژوهش حاضر، بررسی تأثیر 8 هفته تمرین مقاومتی فزآینده و مصرف محلول ساکاروز بر بیان پروتئینهای موثر در لیپولیز بافت عضلۀ اسکلتی موشهای صحرایی بود.
روششناسی: 32 سر موش صحرایی نر بالغ از نژاد ویستار (۶-۸ هفتهای)، ابتدا به طور تصادفی به 2 گروه تغذیه شده با و بدون مصرف محلول ساکاروز 30 درصد تقسیم و پس از 4 هفته هر یک از گروهها به دو گروه تمرین و کنترل تقسیم شدند. گروههای تمرینی به مدت 8 هفته تمرین مقاومتی به روش فزآینده (بالا رفتن از نردبان) را 3 روز در هفته انجام دادند. دو روز پس از آخرین جلسه تمرینی نمونه برداری انجام شد. مقدار پروتئین لیپاز حساس به هورمون (HSL)، گیرندهی کبدی ایکس- آلفا (LXRα) و پریلیپین عضلۀ خم کننده بزرگ انگشتان (FHL) به روش وسترن بلات اندازهگیری شد. جهت تعیین معنادار بودن تفاوت بین متغیرها و تعامل بین آنها از تحلیل واریانس دوطرفه در سطح معنیداری 05/0 P<استفاده شد.
یافتهها: سطوح پروتئین HSL و پریلیپین در آزمودنیهای گروه تمرین در مقایسه با کنترل افزایش (01/0P<)، اما سطح LXRα بدون تغییر بود. تأثیر مصرف محلول ساکاروز به تنهایی و همچنین تعامل بین مصرف محلول ساکاروز و تمرین مقاومتی فزآینده بر بیان پروتئینهای مذکور در بافت عضلۀ معنادار نبود.
نتیجهگیری: نتایج این پژوهش بیانگر آن است که اگرچه 8 هفته تمرین مقاومتی فزآینده موجب افزایش معنادار بیان برخی از پروتئینهای درگیر در لیپولیز شد، لیکن این تغییرات وابسته به مصرف محلول ساکاروز نمیباشد.
کلیدواژهها
عنوان مقاله [English]
The effect of 8 weeks progressive resistance training on the expression of proteins involved in skeletal muscle tissue lipolysis in rats feeding with sucrose solution
نویسندگان [English]
- A Khodamoradi 1
- E Talebi Garakani 2
- F Mir Mohammad Rezaei 3
- R Fathi 2
1 PhD student in Exercise Physiology
2 Associate Professor, Mazandaran University
3 Assistant Professor Professor, Mazandaran University
چکیده [English]
Aim: Excessed energy is stored as triacylglycerol (TAG) in adipose tissue within various organs. In order to reduce excessed fat storage, the utilization of fat storage must be increased to produce energy. The purpose of this study was to evaluate the effects of 8 weeks progressive resistance training on expression of proteins involved in skeletal muscle tissue lipolysis in rats feed with sucrose solution.
Method: 32 male Wistar rats (6-8 weeks) were randomly divided into two groups, the first group took sucrose solution 30%, the second group did not. After 4 weeks, each group divided into exercise and control groups. Exercise groups performed a progressive resistance training protocol 3 days per week for 8 weeks. Tissue samples were taken 2 days after the last session. HSL, Perilipin and LXRα protein expression were determined by Western blot. To evaluate the difference between the variable and the interaction between them, two-way ANOVA at the significant level of P˂0.05 was used.
Results: Protein levels of HSL and Perilipin were increased in the exercise group compared to control (P˂0.001), but LXRα levels were unchanged. The effect of sucrose solution alone and the interaction between the consumption of sucrose and progressive resistance training on the expression of these proteins in muscle tissue were not significant.
Conclusion: The results show that although the 8 weeks of progressive resistance training significantly increased the expression of some proteins involved in lipolysis, but these changes are not related to the consumption of sucrose solution.
کلیدواژهها [English]
- Keywords: Progressive Resistance Training
- Lipolysis
- HSL
- Perilipin
- Liver X Receptor α
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