بررسی تأثیر 6 هفته تمرینات تناوبی شدید بر بیان ژن پروتئین تیروزین فسفاتاز 1B در بافت عضله رت‌های دیابتی نوع‌2

سهیلی, فرشته; عزیزی, محمد; سوری, رحمن; طهماسبی, وریا

doi:10.22124/jme.2017.3379

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I

نویسندگان

¹ دانشجوی کارشناسی ارشد فیزیولوژی ورزشی

² استادیار دانشگاه رازی

³ دانشیار دانشگاه تهران

https://doi.org/10.22124/jme.2017.3379

چکیده

هدف: مقاومت به انسولین نقش مهمی در ایجاد ناهنجاریهایی همچون نقص در تحمل گلوکز، دیابت نوع2، چاقی و هیپرلیپیدمی دارد. کشف پروتئین تیروزین فسفاتاز 1B (PTP-1B) بهنظر میرسد نقطه عطفی در بررسی انتقال سیگنالدهی انسولین است. لذا هدف از پژوهش حاضر، بررسی اثر 6 هفته تمرینات تناوبی شدید (HIIT) بر بیان ژن پروتئین تیروزین فسفاتاز1B در بافت عضله رتهای دیابتی نوع2 میباشد
روش‌شناسی: در این تحقیق، 20 سر رت نر10 هفتهای دیابتی شده به طور تصادفی در دو گروه تمرین تناوبی شدید و کنترل تقسیم شدند. گروه تمرین، برنامه HIIT را هر هفته 5 جلسه 30 دقیقهای به مدت 6 هفته در قالب دویدن روی تردمیل انجام دادند، به طوری که سرعت دویدن به تدریج از 25 متر در دقیقه در هفته اول به 35 متر در دقیقه در هفته ششم افزایش یافت، چهل و هشت ساعت پس از آخرین جلسه تمرین نمونهگیری خونی و بافتبرداری از عضله دوقلوی رتها انجام شد و بیانژن از طریق روش Real-Time PCR انجام شد. از آزمون شپیروویلک و tمستقل در سطح معناداری 05/0P≤ برای تجزیه و تحلیل دادهها استفاده شد.
یافته‌ها: نتایج نشان داد که PTP-1B در رتهای گروه تمرین HIIT کاهش یافت اما از لحاظ آماری معنیدار نبود (432/0=P)، درحالی که شاخص مقاومت به انسولین و گلوکز ناشتا کاهش معنیداری را نشان دادند (001/0=P) اما انسولین سرم بهطور معنیداری افزایش یافت (003/0=P).
نتیجه‌گیری: با توجه به نتایج حاصل، به نظر میرسد تمرین تناوبی شدید با وجود بهبود در دیابت نوع2 و مقاومت به انسولین تأثیری بر بیان ژن PTP-1B نداشته باشد.
واژگان کلیدی: بیان ژن PTP-1B، دیابت نوع2، تمرین تناوبی شدید، مقاومت به انسولین.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of 6 weeks high intensity interval training (HIIT) on expression of PTP-1B in muscle tissue in type 2 diabetic rats

نویسندگان [English]

F Soheili ¹
M Azizi ²
R Soori ³
V Tahmasebi ²

¹ Msc Student in Exercise Physiology

² Assistant Professor, University of Razi

³ Associate Professor, University of Tehran

چکیده [English]

Aim: Insulin resistance plays an important role in the development of abnormalities such as impaired glucose tolerance, type2 diabetes, obesity, and hyperlipidemia. The discovery of protein tyrosine phosphatase 1b (PTP-1B) seems to be a milestone in the investigation of insulin signaling transmission. Thus the purpose of this study was to evaluate the effect of 6 weeks of high intensity interval training (HIIT) on expression of PTP-1B in the muscle tissue in type 2 diabetic rats..
Method: In this study, 20, ten week old diabetic male rats were randomly divided into high intensity interval and control groups. The training group performed training protocol on a treadmill for 6 weeks and 5 session/week, with gradual increase in speed (25-35 m/min) during scheduled runs. Forty-eight hours after the last training session blood and tissue (from gastrocnemius) sampling were carried out in diabetic rats and PTP-1B gene expression was evaluated by Rea-Time PCR method. Data was analyzed using independent t-test and Shapiro Wilk at a significant level of p≤0/05.
Results: Findings show that PTP-1B was decreased in the diabetic rats of HIIT group but was not significant (P=0.432). Also, insulin resistance index and fasting glucose significantly were decreased in HIIT group (P=0.001), but serum insulin was increased (P=0.001).
Conclusion: According to the results, it seems that HIIT does not affect PTP-1B gene expression, but it improves type 2 diabetes and insulin resistance.
Keywords: PTP-1B gene expression, type 2 diabetes, high intensity interval training, insulin resistance.

کلیدواژه‌ها [English]

Keywords: PTP-1B gene expression
type 2 diabetes
High Intensity Interval Training
Insulin resistance

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سوخت و ساز و فعالیت ورزشی

بررسی تأثیر 6 هفته تمرینات تناوبی شدید بر بیان ژن پروتئین تیروزین فسفاتاز 1B در بافت عضله رت‌های دیابتی نوع‌2

مراجع

مراجع

دوره 7، شماره 2
آذر 1396
صفحه 125-134

بررسی تأثیر 6 هفته تمرینات تناوبی شدید بر بیان ژن پروتئین تیروزین فسفاتاز 1B در بافت عضله رت‌های دیابتی نوع‌2

مراجع

مراجع

دوره 7، شماره 2آذر 1396صفحه 125-134

دوره 7، شماره 2
آذر 1396
صفحه 125-134