نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 دانشیار دانشگاه مازندران،
2 دانشجوی دکتری فیزیولوژی ورزشی
چکیده
هدف: دیپپتیدیل پپتیداز-4 (DPP-4) به عنوان پل ارتباطی بین چاقی و مقاومت به انسولین مطرح شده است. هدف از این پژوهش، بررسی تغییرات سطوحسرمی DPP-4 و شاخصمقاومتبهانسولین (HOMA2-IR) پس از هشتهفته تمرینات پیادهروی تناوبی و تداومی در دختران چاق11-9ساله بود.
روششناسی: 32 دختر چاق (با میانگینسنی 75/0±62/9 سال و صدک شاخصتودهبدنی40/1±38/97 درصد) به صورت داوطلبانه در پژوهشحاضر شرکتکردند و در گروههای تمرینتناوبی (12نفر)، تمرینتداومی (11نفر) و کنترل (9نفر) قرارگرفتند. گروههای تمرینی به مدت هشتهفته، 3 جلسه در هفته (30 دقیقه پیادهروی به ترتیب در گروه تداومی و تناوبی با شدت HRmax %60-75 و HRmax %70-85) پرداختند. پیادهروی تناوبی شامل 2 دقیقه پیادهروی و 1 دقیقه استراحتفعال بود. پس از 10 ساعت ناشتایی، نمونهگیری خون در قبل از شروع تمرینات و 72 ساعت پس از آخرینجلسهتمرینی انجامگرفت. برای بررسی تغییرات درونگروهی از آزمونآماری t همبسته و برای بررسی تغییرات بینگروهی، پس از محاسبه اختلاف بین پیشآزمون و پسآزمون از آنالیز واریانس یکطرفه استفاده شد.
یافتهها: هشت هفته تمرینات تداومی و تناوبی نسبت به گروه کنترل، تغییر معنی داری در سطوح DPP-4، انسولین، گلوکز و HOMA2-IR ایجاد نکرد. نسبت به گروه کنترل، تمرین تناوبی موجب بهبودی وزن، شاخصتودهبدنی و صدکشاخصتودهبدنی (بهترتیب 004/0p=، 019/0p= و 022/0p=) شد در حالی که فقط شاخصتودهبدنی در گروه تمرین تداومی تمایل به کاهش داشت (077/0p=).
نتیجهگیری: به نظر میرسد که بهبودی ترکیب بدن ناشی از پروتکل تمرینی مورد استفاده در این پژوهش، برای ایجاد تغییرات معنی دار HOMA2-IR و DPP-4 در دختران چاق نابالغ، کافی نبوده است.
کلیدواژهها
عنوان مقاله [English]
The effect of eight weeks interval and continuous walking on serum levels of dipeptidyl peptidase-4 and insulin resistance index in obese girls aged 9-11 years old
نویسندگان [English]
- R Fathi 1
- M Baghersalimi 2
- M Nazemi 2
- A Khosravi 2
1 Associate Professor, University of Mazandaran
2 Phd student in Exercise Physiology
چکیده [English]
Aim: Dipeptidyl-peptidase-4 (DPP-4) has been proposed as a bridge between obesity and insulin resistance (IR). The aim of this study was to investigate the changes in serum levels of DPP-4 and HOMA2-IR after eight weeks of interval and continuous-walking in 9-11 year-old obese girls.
Method: thirty two-obese girls (age: 9.62±0.75 years and BMI-percentile: 97.38±1.40%) volunteered in this study and were assigned to interval (n=12), continuous (n=11) and control (n=9) groups. The training were performed for 8-weeks, 3-sessions per week (30-min walking in the continuous and interval groups with 60-75%HRmax and 70-85%HRmax, respectively). Interval-walking included 2-min of walking and 1-min of active rest. After 10-hours of fasting, blood sampling was performed before the beginning of training and 72-hours after the last session. The paired t-test was used to examine the within-group changes.
Results: Eight weeks of continuous and interval training did not significantly change the levels of DPP-4, insulin, glucose, and HOMA2-IR compared to the control group. Compared to the control group, the interval training resulted in improvement of the body weight, BMI, and BMI percentile (p=0.004, p=0.019, p=0.022), while the BMI only tended to decrease in the continuous training group (p=0.077).
Conclusion: It seems that the improvement of the body composition due to the protocol used in this study was not sufficient to make significant changes in the HOMA2-IR and DPP-4 in early-pubertal obese girls.
کلیدواژهها [English]
- Keywords: Obesity
- Insulin resistance
- Puberty
- Dipeptidyl-peptidase-4
- Walking
منابع
- عباسی دولویی آ، اسحاقی ر، احمدی م، کهن پور م، ( 1396)، اثر یک دوره تمرین مقاومتی برسطوح سرمی پپتید شبه گلوکاگون-1، دی پپتیدیل پپتیداز-4 و مقاومت انسولین در مردان چاق، فیزیولوژی ورزش و فعالیت بدنی،10: 30-21.
- کاظمی ع، رحمتی م، فاریابی م، طاهرآبادی س ج، (1394)، تاثیر هشت هفته تمرین تناوبی شدید بر وزن بدن و سطوح سرمی TNF-α، انسولین و نیمرخ لیپیدی کودکان دارای اضافه وزن، علوم پزشکی رازی، 22 (139): 7-1.
- Abdul-Ghani MA, Matsuda M, Balas B, and DeFronzo RA. )2007(. Muscle and liver insulin resistance indexes derived from the oral glucose tolerance test. Diabetes care, 30:89-94.
- Alexandraki K, Piperi C, Kalofoutis C, Singh J, Alaveras A, and Kalofoutis A. (2006). Inflammatory process in type 2 diabetes. Ann N Y Acad Sci, 1084:89-117.
- Androutsos O, Grammatikaki E, Moschonis G, Roma‐Giannikou E, Chrousos G, Manios Y, and Kanaka‐Gantenbein C. (2012). Neck circumference: a useful screening tool of cardiovascular risk in children. Pediatr obes, 7:187-195.
- Bird SR, and Hawley JA. (2017). Update on the effects of physical activity on insulin sensitivity in humans. BMJ Open Sport Exerc Med, 2:e000143.
- Cali AM, and Caprio S. (2008). Obesity in children and adolescents. J Clin Endocrinol Metab, 93:s31-s36.
- Caumo A, Perseghin G, Brunani A, and Luzi L. (2006). New insights on the simultaneous assessment of insulin sensitivity and β-cell function with the HOMA2 method. Diabetes Care, 29(12):2733-2734.
- Chen XW, He ZX, Zhou ZW, Yang T, Zhang X, Yang YX, Duan W, and Zhou SF. (2015). Clinical pharmacology of dipeptidyl peptidase 4 inhibitors indicated for the treatment of type 2 diabetes mellitus. Clin Exp Pharmacol Physiol, 42:999-1024.
- Conarello SL, Li Z, Ronan J, Roy RS, Zhu L, Jiang G, Liu F, Woods J, Zycband E, and Moller DE. (2003). Mice lacking dipeptidyl peptidase IV are protected against obesity and insulin resistance. Proc Natl Acad Sci, 100:6825-6830.
- Deacon CF. (2004). Circulation and degradation of GIP and GLP-1. Horm Metab Res, 36:761-765.
- Djalalinia S, Kelishadi R, Qorbani M, Peykari N, Kasaeian A, Nasli-Esfahani E, Naderimagham S, Larijani B, and Farzadfar F. (2016). A Systematic Review on the Prevalence of Overweight and Obesity, in Iranian Children and Adolescents. Iran J Pediatr, 26:e2599.
- Djalalinia S, Moghaddam SS, Peykari N, Kasaeian A, Sheidaei A, Mansouri A, Mohammadi Y, Parsaeian M, Mehdipour P, and Larijani B. (2015a). Mortality attributable to excess body mass Index in Iran: Implementation of the comparative risk assessment methodology. Int J Prev Med, 6:107.
- Djalalinia S, Qorbani M, Peykari N, and Kelishadi R. (2015b). Health impacts of obesity. Pak J Med Sci, 31:239-242.
- Dobrian AD, Ma Q, Lindsay JW, Leone KA, Ma K, Coben J, Galkina EV, and Nadler JL. (2011). Dipeptidyl peptidase IV inhibitor sitagliptin reduces local inflammation in adipose tissue and in pancreatic islets of obese mice. Am J Physiol Endocrinol Metab, 300:410-421.
- Dunmore SJ, and Brown JE. (2012). The role of adipokines in beta-cell failure of type 2 diabetes. J Endocrinol, 216:37-45.
- Farpour-Lambert NJ, Aggoun Y, Marchand LM, Martin XE, Herrmann FR, and Beghetti, M. (2009). Physical activity reduces systemic blood pressure and improves early markers of atherosclerosis in pre-pubertal obese children. J Am Coll Cardiol, 54(25):2396-2406.
- Goran MI, and Sothern MS. (2006). Handbook of pediatric obesity: Etiology, pathophysiology, and prevention: CRC Press. pp. 175-177.
- Kazakos K. (2011). Incretin effect: GLP-1, GIP, DPP4. Diabetes Res Clin Pract, 93:32-36.
- Kelly AS, Wetzsteon RJ, Kaiser DR, Steinberger J, Bank AJ, and Dengel DR. (2004). Inflammation, insulin, and endothelial function in overweight children and adolescents: the role of exercise. J Pediatr, 145(6):731-736.
- Kelly LA, Lane CJ, Weigensberg MJ, Toledo-Corral CM, and Goran MI. (2011). Pubertal changes of insulin sensitivity, acute insulin response, and β-cell function in overweight Latino youth. J Pediatr, 158:442-446.
- Kirino Y, Sato Y, Kamimoto T, Kawazoe K, and Minakuchi K. (2010). Altered dipeptidyl peptidase-4 activity during the progression of hyperinsulinemic obesity and islet atrophy in spontaneously late-stage type 2 diabetic rats. Am J Physiol Endocrinol Metab, 300:372-379.
- Koubaa A, Trabelsi H, Masmoudi L, Elloumi M, Sahnoun Z, Zeghal KM, and Hakim A. (2013). Effect of Intermittent and continuous training on body composition cardio-respiratory fitness and lipid profile in obese adolescents. IOSR J Pharm Biol Sci, 3:31-37.
- Kumar S, and Kelly AS. (2017). Review of Childhood Obesity: From Epidemiology, Etiology, and Comorbidities to Clinical Assessment and Treatment. Mayo Clin Proc, 92(2):251-265.
- Lamers D, Famulla S, Wronkowitz N, Hartwig S, Lehr S, Ouwens DM, Eckardt K, Kaufman JM, Ryden M, and Müller S. (2011). Dipeptidyl peptidase 4 is a novel adipokine potentially linking obesity to the metabolic syndrome. Diabetes, 60:1917-1925.
- Lee SS, Yoo JH, and So YS. (2015). Effect of the low-versus high-intensity exercise training on endoplasmic reticulum stress and GLP-1 in adolescents with type 2 diabetes mellitus. J Phys Ther Sci, 27:3063-3068.
- Lee YS, Biddle S, Chan MF, Cheng A, Cheong M, Chong YS, Foo LL, Lee CH, Lim SC, and Ong WS. (2016). Health promotion board–ministry of health clinical practice guidelines: Obesity. Singapore Med J, 57:292-300.
- Mahgoub MSE-d, and Aly S. (2015). The effects of continuous vs intermittent exercise on lipid profile in obese children. Int J Athl Ther Train, 22:272-276.
- Malin SK, Huang H, Mulya A, Kashyap SR, and Kirwan JP. (2013). Lower dipeptidyl peptidase-4 following exercise training plus weight loss is related to increased insulin sensitivity in adults with metabolic syndrome. Peptides, 47:142-147.
- Marguet D, Baggio L, Kobayashi T, Bernard A-M, Pierres M, Nielsen PF, Ribel U, Watanabe T, Drucker DJ, and Wagtmann N. (2000). Enhanced insulin secretion and improved glucose tolerance in mice lacking CD26. Proc Natl Acad Sci, 97:6874-6879.
- Marson EC, Delevatti RS, Prado AK, Netto N, and Kruel LF. (2016). Effects of aerobic, resistance, and combined exercise training on insulin resistance markers in overweight or obese children and adolescents: A systematic review and meta-analysis. Prev Med, 93:211-218.
- Matthews D, Hosker J, Rudenski A, Naylor B, Treacher D, and Turner R. (1985). Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia, 28(7):412-419.
- Mentlein R. (1999). Dipeptidyl-peptidase IV (CD26)-role in the inactivation of regulatory peptides. Regul Pept, 85:9-24.
- Murphy EC, Carson L, Neal W, Baylis C, Donley D, and Yeater R. (2009). Effects of an exercise intervention using Dance Dance Revolution on endothelial function and other risk factors in overweight children. International Journal of Pediatric Obesity, 4(4):205-214.
- Murphy MH, Nevill AM, Neville C, Biddle S, and Hardman AE. (2002). Accumulating brisk walking for fitness, cardiovascular risk, and psychological health. Med Sci Sports Exerc, 34:1468-74.
- Nassis GP, Papantakou K, Skenderi K, Triandafillopoulou M, Kavouras SA, Yannakoulia M, Chrousos GP, and Sidossis LS. (2005). Aerobic exercise training improves insulin sensitivity without changes in body weight, body fat, adiponectin, and inflammatory markers in overweight and obese girls. Metabolism, 54:1472-1479.
- Pilia S, Casini M, Foschini M, Minerba L, Musiu M, Marras V, Civolani P, and Loche S. (2009). The effect of puberty on insulin resistance in obese children. J Endocrinol Invest, 32:401.
- Pinhas‐Hamiel O, Lerner‐Geva L, Copperman NM, and Jacobson MS. (2007). Lipid and insulin levels in obese children: changes with age and puberty. Obesity, 15:2825-2831.
- Reilly JJ, and Kelly J. (2011). Long-term impact of overweight and obesity in childhood and adolescence on morbidity and premature mortality in adulthood: systematic review. Int J Obes, 35:891-898.
- Reinehr T, Roth CL, Enriori PJ, and Masur K. (2010). Changes of dipeptidyl peptidase IV (DPP-IV) in obese children with weight loss: relationships to peptide YY, pancreatic peptide, and insulin sensitivity. J Pediatr Endocrinol Metab, 23:101-108.
- Röhrborn D, Wronkowitz N, and Eckel J. (2015). DPP4 in diabetes. Front Immunol, 6:e386.
- Sakpal TV. (2010). Sample size estimation in clinical trial. Perspect Clin Res, 1(2):67-69.
- Sell H, Blüher M, Klöting N, Schlich R, Willems M, Ruppe F, Knoefel WT, Dietrich A, Fielding BA, Arner P, and Frayn KN. (2013). Adipose dipeptidyl peptidase-4 and obesity: correlation with insulin resistance and depot-specific release from adipose tissue in vivo and in vitro. Diabetes Care, 36(12):4083-4090.
- Styne DM. (2016). Pediatric Endocrinology: A Clinical Handbook: Springer.
- Tanaka H, Monahan KD, and Seals DR. (2001). Age-predicted maximal heart rate revisited. J Am Coll Cardiol, 37:153-156.
- Tanaka S, Kanazawa I, Notsu M, and Sugimoto T. (2016). Visceral fat obesity increases serum DPP-4 levels in men with type 2 diabetes mellitus. Diabetes Res Clin Pract, 116:1-6.
- Tjønna AE, Lee SJ, Rognmo Ø, Stølen TO, Bye A, Haram PM, Loennechen JP, Al-Share QY, Skogvoll E, and Slørdahl SA. (2008). Aerobic interval training versus continuous moderate exercise as a treatment for the metabolic syndrome. Circulation, 118:346-354.
- Westphal SA. (2008). Obesity, abdominal obesity, and insulin resistance. Clin Cornerstone, 9:23-31.
- Witten K. (2016). Geographies of obesity: environmental understandings of the obesity epidemic. Routledge.
- Yang F, Zheng T, Gao Y, Baskota A, Chen T, Ran X, and Tian H. (2014). Increased plasma DPP4 activity is an independent predictor of the onset of metabolic syndrome in Chinese over 4 years: result from the China National Diabetes and Metabolic Disorders Study. PloS one, 9:e92222.
- You T, Yang R, Lyles MF, Gong D, and Nicklas BJ. (2005). Abdominal adipose tissue cytokine gene expression: relationship to obesity and metabolic risk factors. Am J Physiol Endocrinol Metab, 288:741-747.
- Zilleßen P, Celner J, Kretschmann A, Pfeifer A, Racké K, and Mayer P. (2016). Metabolic role of dipeptidyl peptidase 4 (DPP4) in primary human (pre) adipocytes. Sci Rep, 6:e23074.