نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 دانشجوی دوره دکتر فیزیولوژی ورزشی، گروه تربیت بدنی، واحد لارستان، دانشگاه آزاد اسلامی، لارستان، ایران
2 دانشیار فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد شیراز
3 استادیار فیزیولوژی ورزشی، گروه تربیت بدنی، واحد لارستان ، دانشگاه آزاد اسلامی، لارستان، ایران
4 استادیار فیزیولوژی، گروه فیزیولوژی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
چکیده
مطالعه حاضر با هدف بررسی اثر هشت هفته تمرین استقامتی با شدت متوسط بر بیان ژن هیپوکامپی عامل نوروتروفیک مشتق از مغز و گیرنده تیروزین کیناز B در موش های صحرایی آلزایمری شده با نوروتوکسین تریمتیلتین انجام شد.
در این مطالعه 24 سر موش صحرایی نر بالغ از نژاد اسپراگ داولی با سن هشت هفته با تزریق درون صفاقی mg/kg 8، سم تریمتیلتین کلراید مبتلا به بیماری آلزایمر شده و در گروههای (1) کنترل مبتلا به آلزایمر هفته اول، (2) کنترل مبتلا به آلزایمر هفته آخر، و (3) تمرین استقامتی+TMT تقسیم شدند. جهت بررسی اثرات القاء بیماری آلزایمر و گذر هشت هفته عمر بر متغیرهای تحقیق 61 سر موش صحرایی سالم در گروه کنترل هفته اول و کنترل هفته آخر قرار گرفتند. موش‌های صحرایی گروه تمرین استقامتی به مدت هشت هفته، سه جلسه در هفته، هر جلسه 51 تا 03 دقیقه و با سرعت 51 تا 02 متر بر دقیقه بر نوارگردان ویژه موش‌های صحرایی دویدند
نتایج نشان داد بیان ژن BDNF و گیرنده TrkB در گروه کنترل آلزایمر هفته اول به طور معنی داری کمتر از گروه سالم هفته اول بود؛ همچنین تفاوت معنی‌داری در بیان ژن هیپوکامپی BDNF و گیرنده TrkB بین گروه کنترل مبتلا به بیماری آلزایمر قربانی هفته آخر در مقایسه با گروه تمرین استقامتی وجود ندارد.
به نظر میرسد تمرین استقامتی با شدت و مدت به کار رفته در تحقیق حاضر اثر معنی‌داری بر تغییرات بیان ژن هیپوکامپی FNDB و گیرنده BKrT در موش‌های صحرایی مدل دژنراسیون هیپوکامپ ندارد.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of eight weeks moderate-intensity endurance training on hypocampic brain-derived neurotrophic factor and tyrosine kinase B receptor gene expression in the rats with hippocampal degeneration model
نویسندگان [English]
- Fatemeh Akbari 1
- mehrzad moghadasi 2
- Sirus Farsi Farsi 3
- Mohammad Amin Edalatmanesh 4
1 Department of exercise physiology, Larestan branch, Islamic Azad University, Larestan, Iran
2 Associate professor in exercise physiology, Islamic Azad University, Shiraz branch
3 Assistant professor in exercise physiology, Department of exercise physiology, Larestan branch, Islamic Azad University, Larestan, Iran
4 Assistant professor in physiology, Department of physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran
چکیده [English]
The purpose of the present study was to examine the effect of eight weeks moderate-intensity endurance training on hypocampic brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB) receptor gene expression in the rats with hippocampal degeneration model.
In this experiment, twenty four mature Sprague-dawley male rats were subjected to Alzheimer’s disease through intraperitoneally injection of 8 mg/kg Trimethytin (TMT) and then were divided into (1) Alzheimer-infected control group, (2) endurance training, and (3) sham to study the impact of the disease on the variables. Sixteen healthy rats were assigned to the control group that eight rats sacrifice at first week and eight rats sacrifice at last week. The rats in the endurance training group ran on a rat treadmill with the speed of 15 to 20 meters per minute for 15 to 30 minutes in each session, 3 times a week for 8 weeks. To analyze the results of the tests, one-way ANOVA and Tukey post hoc test were run using SPSS
The results revealed that induction of Alzheimer’s disease by TMT decreases the BDNF and TrkB receptor gene expression in rats. The results, also indicated that there were no significant differences in hypocampic BDNF and TrkB receptor gene expression between endurance training group and sham group.
According to the study results, it seems that endurance training with specific intensity and duration utilized in this study had not significant effect on changes of hypocampic BDNF and TrkB receptor gene expression in the rats with hippocampal degeneration model.
کلیدواژه‌ها [English]
- Endurance training
- Brain-derived neurotrophic factor
- Tyrosine kinase B receptor
- Hippocampus
- Alzheimer disease
- Albeck DS, Sano K, Prewitt GE, Dalton L. (2006). Mild forced treadmill exercise enhances spatial learning in the aged rat. Behav Brain Res, 168: 345-348.
- Arvin H, Tavakol L. (2017). Effect of 8 weeks selected Spark Motor Program on brain derived neurotrophic factor in intellectually disabled educable boys. J Physic Act Horm, 1: 13-22.
- Baziyar Y, Edalatmanesh MA, Hosseini SA, Zar A. (2016). The effects of endurance training and gallic acid on BDNF and TNF-α in male rats with Alzheimer. Int J Appl Exerc Physiol, 5: 45-54.
- Cummings J, Lee G, Ritter A, Sabbagh M, Zhong K. (2019). Alzheimer’s disease drug development pipeline: 2019. Alzheimer’s Dement (New York, N Y), 5: 272-293.
- Dabidi, R. V., Hosseinzadeh, S., Mahjoub, S., Hosseinzadeh, M., & Myers, J. (2013). Endurance exercise training and diferuloyl methane supplement: changes in neurotrophic factor and oxidative stress induced by lead in rat brain. Biology of sport, 30(1), 41–46.
- De la Rosa A, Solana E, Corpas R, Bartrés-Faz D, Pallàs M, Vina J, et al. (2019). Long-term exercise training improves memory in middle-aged men and modulates peripheral levels of BDNF and Cathepsin B. Sci Rep, 9: 3337.
- Eremenko E, Mittal K, Berner O, Kamenetsky N, Nemirovsky A, Elyahu Y, et al. (2019). BDNF-producing, amyloid β-specific CD4 T cells as targeted drug-delivery vehicles in Alzheimer’s disease. EBioMedicine, 43: 424-434.
- Ferreira AFB, Real CC, Rodrigues AC, Alves AS, Britto LRG. (2011). Short-term, moderate exercise is capable of inducing structural, BDNF-independent hippocampal plasticity. Brain Res, 1425: 111-122.
- Fonseca-Gomes J, Jerónimo-Santos A, Lesnikova A, Casarotto P, Castrén E, Sebastião AM, et al. (2019). TrkB-ICD fragment, originating from BDNF receptor cleavage, is translocated to cell nucleus and phosphorylates nuclear and axonal proteins. Front Mol Neurosci, 12: 4.
10. Houlton J, Zhou LYY, Barwick D, Gowing EK, Clarkson AN. (2019). Stroke induces a BDNF-dependent improvement in cognitive flexibility in aged mice. Neural Plast, 2019: 1460890.
11. Iuliano E, di Cagno A, Cristofano A, Angiolillo A4, D'Aversa R4, Ciccotelli S, et al. (2019). Physical exercise for prevention of dementia (EPD) study: background, design and methods. BMC Public Health, 19: 659.
12. Kaur S, Sharma N, Nehru B. (2018). Anti-inflammatory effects of Ginkgo biloba extract against trimethyltin-induced hippocampal neuronal injury. Inflammopharmacology, 26: 87-104.
13. Lee Y Il, Kim YG, Pyeon HJ, Ahn JC, Logan S, Orock A, et al. (2019). Dysregulation of the SNARE-binding protein Munc18-1 impairs BDNF secretion and synaptic neurotransmission: a novel interventional target to protect the aging brain. GeroScience, 41: 109-123.
14. Luo L, Li C, Deng Y, Wang Y, Meng P, Wang Q. (2019). High-intensity interval training on neuroplasticity, balance between brain-derived neurotrophic factor and precursor brain-derived neurotrophic factor in poststroke depression rats. J Stroke Cerebrovasc Dis, 28: 672-682.
15. Mitra S, Behbahani H, Eriksdotter M. (2019). Innovative therapy for Alzheimer’s disease-with focus on biodelivery of NGF. Front Neurosci, 13: 38.
16. Ng TKS, Ho CSH, Tam WWS, Kua EH, Ho RC-M. (2019). Decreased serum brain-derived neurotrophic factor (BDNF) levels in patients with Alzheimer’s disease (AD): A Systematic Review and Meta-Analysis. Int J Mol Sci, 20: 257.
17. Prpar Mihevc S, Majdič G. (2019). Canine cognitive dysfunction and Alzheimer’s disease - two facets of the same disease? Front Neurosci, 13: 604.
18. Rahmati-Ahmadabad S, Azarbayjani M, Nasehi M. (2017). The effects of high-intensity interval training with supplementation of Ffaxseed oil on BDNF mRNA expression and pain feeling in male rats. Ann Appl Sport Sci, 5: 1-12.
19. Roh, H. T., & So, W. Y. (2017). The effects of aerobic exercise training on oxidant-antioxidant balance, neurotrophic factor levels, and blood-brain barrier function in obese and non-obese men. Journal of sport and health science, 6(4), 447–453.
20. Salehi OR, Hosseini SA, Farkhaie F, Farzanegi P, Zar A. (2019). The effect of moderate intensity endurance training with genistein on brain-derived neurotrophic factor and tumor necrosis factor-α in diabetic rats. J Nutr Fasting Heal, 7: 44-51.
21. Sanders, L. M. J., Hortobágyi, T., Karssemeijer, E. G. A., Van der Zee, E. A., Scherder, E. J. A., & Van Heuvelen, M. J. G. (2020). Effects of low-and high-intensity physical exercise on physical and cognitive function in older persons with dementia: a randomized controlled trial. Alzheimer's research & therapy, 12(1), 1-15.
22. Soya H, Nakamura T, Deocaris CC, Kimpara A, Iimura M, Fujikawa T, et al. (2007). BDNF induction with mild exercise in the rat hippocampus. Biochem Biophys Res Commun, 358: 961-967.
23. Tari AR, Norevik CS, Scrimgeour NR, Kobro-Flatmoen A, Storm-Mathisen J, Bergersen LH, et al. (2019). Are the neuroprotective effects of exercise training systemically mediated? Prog Cardiovasc Dis, 62: 94-101.
24. Zhang Z, Wang B, Fei A. (2019). BDNF contributes to the skeletal muscle anti-atrophic effect of exercise training through AMPK-PGC1α signaling in heart failure mice. Arch Med Sci, 15: 214-222.
)