نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I

نویسندگان

1 گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

2 استادیار گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

3 گروه فیزیولوژی ورزشی، دانشگاه گیلان، رشت، ایران

4 گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل

چکیده

هدف: تبدیل بافت چربی سفید (WAT) به بافت چربی قهوه‌ای (BAT) یکی از روش‌های بالقوه درمان چاقی می‌باشد. هدف از پژوهش حاضر بررسی اثر تمرین هوازی و کپسایسین بر بیان FNDC5 و مقادیر پروتئینی آیریزین بافت چربی احشایی در موش‌های تغذیه شده با رژیم غذایی پرچرب (HFD) می‌باشد. روش‌ شناسی: 40 سر موش صحرایی نر ویستار به مدت 8 هفته با رژیم غذایی نرمال (8=n، ND) و HFD (32=n) تغذیه شدند. بعد از هشت هفته همه موش‌ها به 5 گروه: ND، HFD، رژیم غذایی پرچرب-تمرین (HFDT)، رژیم غذایی پرچرب-کپسایسین (HFDCap) و رژیم غذایی پرچرب-تمرین- کپسایسین (HFDTCap) تقسیم شدند. گروه‌های تمرین به مدت هشت هفته برنامه تمرین هوازی فزاینده (25-15 متر در دقیقه، 60-30 دقیقه در روز، پنج روز در هفته) را روی تردمیل انجام دادند. کپسایسین (mg/kg/day 4) یک بار در روز به صورت خوراکی با گاواژ خورانده شد. یافته‌ها: نتایج پژوهش حاضر نشان‌دهنده کاهش بیان FNDC5 (001/0p=) و آیریزین (000/0p=) در گروه HFD نسبت به گروه ND بود. همچنین میزان بیان FNDC5 و آیریزین در گروه‌های HFDT (به ترتیب 006/0p=، 020/0p=)، HFDCap (به ترتیب 005/0p=، 021/0p=) و HFDTCap (به ترتیب 000/0p=، 000/0p=) نسبت به HFD افزایش معنی‌داری داشت. میزان بیان FNDC5 و آیریزین در HFDTCap نسبت به گروه‌های HFDT (به ترتیب 037/0p=، 035/0p=) و HFDCap (به ترتیب 044/0p=، 033/0p=) نیز افزایش معنی داری داشت. بحث: تمرین هوازی همراه با مصرف کپسایسین با افزایش FNDC5 و آیریزین در بافت چربی احشایی، اثر مضاعفی بر عوامل موثر بر قهوه‌ای کردن بافت چربی داشت.

کلیدواژه‌ها

عنوان مقاله [English]

Synergistic Effects of Aerobic Training and Capsaicin on FNDC5 Gene Expression and Irisin Protein Level of Visceral Adipose Tissue in Rat Fed a High-Fat Diet

نویسندگان [English]

  • Maryam Mostafavian 1
  • Ahmad Abdi 2
  • Javad Mehrabani 3
  • Alireza Barari 4

1 Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, amol, I. R. Iran

2 Assistant Professor, Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.

3 Department of Exercise Physiology, Univercity of Guilan, Rasht, Iran

4 Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, I. R. Iran

چکیده [English]

Aim: Converting white adipose tissue (WAT) to brown adipose tissue (BAT) is therapeutic potential to combat obesity. The purpose of this study was to investigate the effects of aerobic training and capsaicin (Cap) on FNDC5 gene expression and irisin protein level of visceral adipose tissue in rat fed a high-fat diet (HFD).
Methods: 40 male Wistar rats were fed a normal diet (ND, n = 8) or HFD (n = 32) for 8 weeks. After 8 weeks, all rats were divided into 5 groups: ND, HFD, high-fat diet-training (HFDT), high-fat diet-capsaicin (HFDCap), high-fat diet-training-capsaicin (HFDTCap). Training groups have performed a progressive aerobic running program (at 15-25 m/min, 30-60 min/day, and 5 days/week) on a motor-driven treadmill for eight weeks. Capsaicin (4 mg/kg/day) were administered orally, by gavage, once a day.
Results: The results of this study showed that FNDC5 expression (p=0.001) and irisin (p=0.000) was decreased in HFD group compared to ND group. Also, the expression of FNDC5 and irisin in HFDT (Respectively p=0.006, p=0.020), HFDCap (Respectively p=0.005, p=0.021) and HFDTCap (Respectively p=0.000, p=0.000) groups was significantly increased compared to HFD. The expression of FNDC5 and irisin in HFDTCap was also significantly increased compared to HFDT (Respectively p=0.037, p=0.035) and HFDCap (Respectively p=0.044, p=0.033) groups.
Conclusions: Aerobic training combination with capsaicin with increased FNDC5 and irisin in visceral adipose tissue, had Interactive effects on factors affecting browning of adipose tissue.

کلیدواژه‌ها [English]

  • Exercise
  • Capsaicin
  • FNDC5
  • Irisin
  • obesity
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