نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

2 استادیار، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

3 دانشیار، گروه فیزیولوژی ورزشی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

4 دانشیار فیزیولوژی ورزشی، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

5 استادیار، گروه دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

چکیده

هدف: شامل بررسی تاثیر توام هشت هفته تمرین HIIT و مکمل کورکومین بر مقدار مرگ سلولی و GSH سلولهای کبدی در موشهای نر تحت مواجهه با آرسنیک آب آشامیدنی بود.
روش شناسی: تعداد 48 سر رت نر به شش گروه شامل تمرین، کورکومین، توام(تمرین و کورکومین)، آرسنیک، کنترل اتانول و کنترل آب مقطر تقسیم‌ شدند. آرسنیک به مدت شش هفته روزانه از طریق آب آشامیدنی با دوز 5 میلی‌گرم بر هر کیلوگرم و کورکومین روزانه 15 میلی‌گرم بر هر کیلوگرم وزن بدن به صورت گاواژ مصرف شدند. پروتکل HIIT به مدت شش هفته (، 5 روز در هفته) شامل 60 دقیقه دویدن تناوبی(4 دقیقه دویدن با شدت 90-85 درصد VO2max و 2 دقیقه ریکاوری فعال با شدت 60 – 50 درصد) انجام شد.
یافته ها: مواجهه با آرسنیک سبب کاهش مقدار CK18 M65 و کاهش نسبتا مختصر مقدار GSH کبد شد. هم کورکومین و هم اثر توام سبب معکوس شدن تغییرات CK18 M65 کبد شدند، اما فقط در گروه توام افزایش محسوس مقدار GSH کبد مشاهده شد.
نتیجه‌گیری: مواجهه با آرسنیک کبد را در معرض استرس اکسایشی و آپوپتوز قرار می دهد و هم تمرین و هم کورکومین اثار قابل ملاحظه ای در معکوس کردن این اثرات دارند. اما فقط اثر توام قادر به جبران ذخایر ضداکسایشی کبد در مواجهه با آرسنیک می باشد. بااینحال، به دلیل محدودیتهای تحقیق و کمبود شواهد مشابه، همچنان نیاز به بررسی‌های بیشتر باقی است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effects of HIIT and curcumin supplementation on plasma CK18 and hepatic GSH level in rats exposed to arsenic

نویسندگان [English]

  • nooshin salehi aghdam 1
  • Roghayeh Pouzesh Jadidi 2
  • Karim Azali Alamdari 3
  • jabbar bashiri 4
  • Mir Ali Reza Nourazar 5

1 PHD student of Exercise Physiology, Department of Physical Education, Tabriz branch, Islamic Azad University, Tabriz, Iran

2 : Assistant Prof., Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran

3 : Associate Professor of Exercise Physiology, Azarbaijan Shahid Madani University, Tabriz, Iran,

4 : Associate Prof., Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz

5 Assistant Prof., Department of Vet, Tabriz branch, Islamic Azad University

چکیده [English]

Aim: we investigated the effects of six weeks of HIIT and curcumin supplementation on hepatic cellular death and also GSH level in male rats exposed to drinking water arsenic.
Methods: 48 rats were randomized in six groups including HIIT, curcumin, HIIT+curcumin, arsenic, ethanol control and normal control. Arsenic 5 mg/bw.day and curcumin 15 mg/bw.day, were consumed orally for entire the study period. HIIT were conducted for six weeks (5 d/w, 60 min/session (with 4 min running at 85-90% of Vo2max and 2 min recovery at 50-60% of Vo2max intervals).
Results: Arsenic exposure down regulated hepatic CK18 M65 level and also in a lesser extent; the cellular GSH content. Curcumin supplementation either alone or concomitantly with HIIT, could reverse the changes in hepatic CK18 M65 level. However; only in the HIIT+curcumin group, a remarkable elevation of hepatic GSH level was observed.
Conclusion: Arsenic exposure renders liver to an increased oxidative stress and apoptosis level and the both HIIT and curcumin supplementation have appreciable effects to neutralize these hazardous effects. Moreover, only the synergistic effect of HIIT and curcumin supplementation could attenuate the liver antioxidants following arsenic exposure. However, more investigations remains to be done because of the study limitations and lack of similar evidence in this area.

کلیدواژه‌ها [English]

  • Arsenic
  • HIIT
  • Curcumin
  • Liver
  • Apoptosis

   

 

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

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