نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسنده
گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت/ایران
چکیده
هدف : در این مطالعه سطح آنزیم‌های پیرووات‌دهیدروژنازکیناز 4 (PDK-4) و کارنیتین‌پالمیتوئیل‌ترنسفراز 1 (CPT-1) و ارتباط آن‌ها با سطوح گلوکز، لاکتات و نیم‌رخ لیپید خون در پاسخ به یک جلسه فعالیت کراس‌فیت در شرایط ناشتایی و سیری مورد بررسی قرار گرفت.
روش پژوهش: 23 دانشجوی علوم ورزشی زن سالم با حداقل 3 سال سابقه تمرین منظم به شکل تصادفی در 4 گروه کنترل ناشتا، کنترل سیر، کراس‌فیت ناشتا و کراس‌فیت سیر قرار گرفتند. در پایان جلسه میزان سختی کار با مقیاس OMNI سنجیده شده و نمونه‌گیری خون وریدی از آزمودنی‌ها انجام شد. از آزمون‌های آنالیز واریانس دو سویه و همبستگی پیرسون در سطح آلفای 05/0 در SPSS 22 برای بررسی فرضیه‌های پژوهش استفاده گردید.
یافته‌ها: میانگین سطوح گلوکز، تری‌گلیسرید، کلسترول، LDL، HDL، PDK-4 و CPT-1 بین گروه‌‌ها تفاوت معنی‌داری نداشت (05/0 < P). سطح پلاسمایی لاکتات در گروه‌های فعالیت ورزشی کراس‌فیت نسبت به گروه‌های کنترل بالاتر بود (045/0 = P). سطح سرمی PDK-4 ارتباط مثبت معنی‌دار با CPT-1 (007/0 = P، 550/0 = r) و ارتباط منفی معنی‌داری با سطح پلاسمایی لاکتات داشت (003/0 = P، 600/0 - = r).
نتیجه‌گیری: وضعیت سیری و ناشتایی تأثیری بر شاخص‌های متابولیک مد نظر در حالت استراحت و فعالیت نداشت. سطوح بالاتر لاکتات پس از فعالیت ورزشی کراس‌فیت و عدم تفاوت سطوح PDK-4 و CPT-1 با وضعیت استراحتی، احتمالا نشان از سهم بیشتر گلوکز در تولید انرژی از مسیرهای هوازی و بی‌هوازی در این پروتکل دارد.
کلیدواژه‌ها
عنوان مقاله [English]
Effect of a single session of Crossfit exercise in fasting and fed state on PDK4 and CTP-1 levels and their relationship with blood glucose, lactate and lipid profile in active female students
نویسنده [English]
- Maryam Ebrahimi
department of exercise physiology, Faculty of physical education and sport sciences, University of Guilan, Rasht/Iran
چکیده [English]
Objective: in this research, levels of metabolic enzymes, pyruvate dehydrogenase kinase 4 (PDK-4) and carnitine palmitoyltransferase 1 (CPT-1) and their relationship with blood glucose, lactate and lipid profile were studied in response to a single bout of Crossfit exercise in fasting and fed state.
Methods: 23 healthy female sport science students with minimum of 3 years regular training were randomly assigned into fast control, fed control, fast Crossfit and fed Crossfit groups. At the end of the session, rating of perceived exertion was estimated by OMNI scale and blood samples were collected. Two-way analysis of variances and Pearson test were used at the alpha level .05 in SPSS 22.
Results: glucose, TG, cholesterol, LDL, HDL, PDK-4 and CPT-1 mean levels had not any significant differences between groups (P > .05). Plasma lactate level was higher in Crossfit groups compared to controls (P = .045). Serum PDK-4 was positively correlated with CPT-1 (r = .550, P = .007) and negatively correlated with lactate levels (r = .600, P = .003).
Conclusion: fasting and feeding had not any effect on metabolic indices, nor in resting neither after exercise. Higher lactate levels after Crossfit exercise and no difference in PDK-4 and CPT-1 compared with resting, probably suggest more contribution of glucose for energy production via aerobic and anaerobic pathways in this protocol.
کلیدواژه‌ها [English]
- metabolic enzymes
- functional training
- metabolism
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