نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 دانشجوی دکترای تخصصی فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
2 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
3 دانشیار ، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
چکیده
هدف از پژوهش حاضر بررسی اثر تمرینات مقاومتی–هوازی بر رشد تارهای عضله نعلی، سرم تستوسترون و گیرنده‌های آندروژنی عضله موش‌های صحرایی نر با رده‌های سنی مختلف می باشد.30 سر موش صحرایی نر نژاد ویستار کودک 2 هفته ای (10n=)، جوان 6 هفته ای (10n=) و سالمند 96 هفته ای (10n=) در دو گروه تمرین و کنترل قرار گرفتند.گروه تمرین بعد از یک هفته آشنایی در برنامه 6 روز تمرین در هفته به طور متناوب یک روز مقاومتی و یک روز هوازی به مدت 6 هفته شرکت کردند. موش‌ها 48 ساعت پس از آخرین جلسه تمرینی بیهوش و بعد از خونگیری کشته و عضله دو قلو و نعلی استخراج شدند. همچنین سرم خون جداسازی شد و سپس غلظت تستوسترون خون به روش الایزا، رشد عضلانی توسط میکروسکوپ نوری و بیان ژنی گیرنده های آندروژنی عضله نعلی به روش Real Time PCR اندازه‌گیری گردید. نتایج نشان داد سطح مقطع تارهای عضلانی گروه های تمرین کودک (001/0=p)، جوان (003/0=p) و سالمند (001/0=p) نسبت به گروه‌های کنترل بیشتر بود. بین تستوسترون سرم گروه تمرین و کنترل رده سنی کودک تفاوت معنی-داری وجود نداشت (6/0=p). در حالی‌که تستوسترون سرم گروه‌های تمرین در هر دو رده سنی جوان (008/0= p) و سالمند (001/0=p) بیشتر از گروه‌های کنترل بود. همچنین در بیان گیرنده‌های آندروژنی، تفاوت معنی‌داری بین گروه‌های تمرین و کنترل کودک (3/0=p)، جوان (3/0=p) و سالمند (8/0=p) مشاهده نشد. تمرین مقاومتی-هوازی می تواند با افزایش غلظت تستوسترون باعث هایپرتروفی عضلانی در سنین مختلف گردد و با تسریع فرایند رشد مانع از آتروفی عضلانی در سالمندی شود.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of resistance-aerobic training on the growth of muscle fibers, serum testosterone and androgen receptors on soleus muscles, in different ages groups of male rats
نویسندگان [English]
- Mohammad Mahdi Bagherpour Tabalvandani 1
- Mohammad Reza Fadaei 2
- Alireza Elmieh 3
1 Ph.D. Candidate of Exercise Physiology, Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran.
2 Assistant Professor, Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran.
3 Associate Professor, Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran.
چکیده [English]
The aim of this study was to evaluate the effect of aerobic resistance training on the growth of muscle fibers, testosterone and androgen receptors in male rats of different age groups. 30 male Wistar rats, child (n=10), young (n=10) and elderly (n=10), were divided into two groups of training and control Exercise were performed intermittently, one day for resistance and one day for aerobic for 6 weeks. 48 hours after the last training session, anesthesia, blood sampling and then were killed and then gastrocnemius and soleus muscles were extracted. Blood serum was also isolated and then blood testosterone was measured by ELISA, muscle growth was measured by light microscopy and gene expression of androgen receptors was measured using Real-time pcr. The results showed that the cross- sectional of muscle fiber in all three training groups of ages contains: child (p=0.001), young (p=0.003) and elderly (p=0.001) were more than control groups. There were no significant difference in serum testosterone between training and control groups of child (p=0.6), while serum testosterone in young (p=0.008) and elderly (p=0.001) training groups were more than controls. Also there were no significant difference in expression of androgen receptors between training and control groups of child (p=0.3), young (p=0.3) and elderly (p=0.8). The results showed that resistance-aerobic exercise can cause muscle hypertrophy at different ages by increasing testosterone concentration. Resistance-aerobic training can accelerate the growth process and also prevent muscle atrophy in old age.
کلیدواژه‌ها [English]
- Hypertrophy
- Testosterone
- Androgen Receptor
- Different Ages
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