نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزشی، گروه تربیت‌بدنی، واحد علی‌آبادکتول، دانشگاه آزاد اسلامی، علی‌آبادکتول، ایران

2 2دکترای فیزیولوژی ورزشی، گروه تربیت‌بدنی، واحد علی‌آبادکتول، دانشگاه آزاد اسلامی، علی‌آبادکتول، ایران

3 استادیار، گروه تربیت‌بدنی، واحد علی‌آبادکتول، دانشگاه آزاد اسلامی، علی‌آبادکتول، ایران

4 استادیار، گروه تربیت بدنی و علوم ورزشی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

هدف: مطالعه حاضر به بررسی تأثیر تمرین هوازی تناوبی به همراه مصرف مکمل سیلی‌مارین بر شاخص‌های بافتی آنتی‌اکسیدانی، آسیب کبدی و آتروژنیک رت‌های نر ویستار تغذیه شده با غذای پرچرب پرداخت.
روش بررسی: مطالعه حاضر از نوع تجربی و روش آزمایشگاهی بود. بدین منظور، 35 سر رت نر نژاد ویستار با سن سه هفته و وزن 08/7±45/160 گرم، به روش تصادفی به 5 گروه‌ 7 سری شامل کنترل غذای معمولی (ODC)، کنترل غذای چرب (FDC)، غذای چرب+مکمل (FDS)، غذای چرب+تمرین (FDE)، غذای چرب+تمرین+مکمل (FDSE) تقسیم شدند. برنامه تمرینی شامل 8 هفته، 5 جلسه 30 دقیقه‌ای فعالیت روی نوارگردان در هفته بود. مکمل سیلی‌مارین با دوز 140 میلی‌گرم/کیلوگرم/روزانه نسبت به وزن بدن به مدت دو هفته گاواژ می‌‌شد. 48 ساعت پس از آخرین جیره غذایی، نمونه‌ بافتی و خون‌گیری انجام و داده‌ها تحلیل شدند.
یافته‌ها: متغیرهایALT، AST، ALP، MDA، TC و TG در گروه‌های غذای چرب+تمرین+مکمل و غذای چرب+تمرین نسبت به گروه کنترل غذای چرب کاهش معنی‌داری داشتند. در حالی‌‌که متغیرهای SOD و HDL-C، تنها در گروه غذای چرب+تمرین+مکمل نسبت به کنترل غذای چرب افزایش معنی‌داری داشت. متغیرهای LDL-C و AIP نیز تنها در گروه غذای چرب+تمرین+مکمل نسبت به کنترل غذای چرب کاهش معنی‌داری داشتند (05/0>p).
نتیجه‌گیری: استفاده از تمرین هوازی تناوبی به تنهایی یا با مصرف مکمل سیلی‌مارین، سبب کاهش متغیرهای خطرزای بیماری کبد چرب غیرالکلی از قبیل آنزیم‌های درگیر و شاخص آتروژنیک در موش‌های تغذیه‌شده با غذای پرچرب می‌شود؛ بنابراین می-تواند از طریق افزایش عملکرد آنتی‌اکسیدانی و کاهش پروفایل چربی‌های خون، به بهبود عارضه موردنظرکمک نماید.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of 8 weeks of aerobic interval exercise with silymarin supplementation on antioxidant, liver damage and atherogenic indicators in male Wistar rats fed a high-fat diet

نویسندگان [English]

  • Masoud Shakki 1
  • Fatemeh Hosseini 2
  • Saeed Ghorbani 3
  • reza rezai shirazi 3
  • H parsian 4

1 MSc in sport physiology, Department of Physical Education, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

2 PhD in sport physiology, Department of Physical Education, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

3 Assistant Professor, Department of Physical Education, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

4 Assistant Professor, Department of Physical Education and sport science, Shah-e-Qods Branch, Islamic Azad University, Tehran, Iran

چکیده [English]

Aim: The purpose of the study was to investigate the effect of 8 weeks of aerobic interval exercise with silymarin supplementation on antioxidant, liver damage and atherogenic indicators in male Wistar rats fed a high-fat diet.
Methods: This experimental study with laboratory method was performed on 35 male Wistar rats with a body weight of 160.45±7.08g and 3-weeks-of-age were randomly divided into 5 groups consisted of ordinary diet control (ODC), fatty diet control (FDC), fatty diet with supplement (FDS), fatty diet with exercise (FDE), fatty diet with supplement and exercise (FDSE). The exercises included the running on treadmill for 8 weeks, five times/week and 30 minutes in an exercise session. Silymarin supplementation with dose of 140 mg/kg/day of body, weight was received for two weeks. Liver tissue and samples were obtained after 48 hours of the last diet and data analyzed.
Results: Significant decrease in FDSE and FDE groups compared with FDC group in ALT, AST, and ALP, MDA, TC and TG variables were observed. Whereas, there were significantly increased in FDSE group compared with FDC in SOD and HDL-C variables. Also, LDL-C and AIP in FDSE group compared with FDC had shown a significant decrease (p> .05).
Conclusion: The applying of aerobic interval exercise alone or with silymarin supplementation reduced the risk to NAFLD such as enzymes involved and atherogenic index in male Wistar rats fed a high-fat diet; therefore, it could probably improve the disease through increase the antioxidant capacity and reduce blood lipid profiles.

کلیدواژه‌ها [English]

  • Aerobic Interval Exercise
  • Silymarin
  • Antioxidant
  • Atherogenic Index
  • Nonalcoholic Fatty Liver
  1. Abdi Ardekani M, Banaeifar A, Arshadi S, Abed Natanzi H. (2020). Effect of High-Intensity Interval Training and Thyme honey on liver enzymes of type II diabetic rats. J Diabetes Nurs. 8(3):1160-1174.
  2. Abdi F, Atarodi KZ, Mirmiran P, Esteki T. (2015). Surveying global and Iranian food consumption patterns: A review of the literature. J Fasa Univ Med Sci, 5 (2):159-167.
  3. Barua L, Faruque M, Banik PC, Ali L. (2019). Atherogenic index of plasma and its association with cardiovascular disease risk factors among postmenopausal rural women of Bangladesh. Indian Heart Journal. 71(2):155-160.
  4. Berná G, Romero‐Gomez M. (2020). The role of nutrition in non‐alcoholic fatty liver disease: Pathophysiology and management. Liver International. 40:102-108.
  5. Chikezie CM, Ojiako OA, Emejulu AA, Chikezie PC. (2018). Atherogenicity of diabetic rats administered single and combinatorial herbal extracts. Bulletin of Faculty of Pharmacy, Cairo University. 56(2):169-174.
  6. Da Silva MR, Waclawovsky G, Perin L, Camboim I, Eibel B, Lehnen AM. (2020). Effects of high-intensity interval training on endothelial function, lipid profile, body composition and physical fitness in normal-weight and overweight-obese adolescents: A clinical trial. Physiology & Behavior. 213:112728.
  7. Fallah-Hoseini H, Ardeshir-Larijani M, Rajabipoor B, Heshmat R. (2004). The effect of silymarin in the treatment of type 2 diabetes A double-blind clinical trial study. Medicinal Plants. 4(Special issue of thistle):13-17. [In Persian]
  8. Fraschini F, Demartini G, Esposti D. (2002). Pharmacology of Silymarin. Clinical Drug Investigation. 22(1):51-65.‏
  9. Giuseppe D, Angela DA, Davide R, Pamela M. (2017). Effects of a combination of berberis aristata, silybum marianum and monacolin on lipid profile in subjects at low cardiovascular risk; a double-blind, randomized, placebo-controlled trial. International Journal of Molecular Sciences. 18(2):343.
  10. Groussard C, Maillard F, Vazeille E, Barnich N, Sirvent P, Otero YF, Combaret L, Madeuf E, Sourdrille A, Delcros G, Etiene M, Teixeira A, Sauvanet P, Pialoux V, Boisseau N. (2019). Tissue-specific oxidative stress modulation by exercise: A comparison between MICT and HIIT in an obese rat model. Oxidative Medicine and Cellular Longevity, 2019.
  11. Heidarian E, Nouri A. (2019). Hepatoprotective effects of silymarin against diclofenac-induced liver toxicity in male rats based on biochemical parameters and histological study. Archives of Physiology and Biochemistry: 1-7.
  12. Homayoonfar R, Ahram-Poosh E, Koohpaye SA, Meshkibaf MH, Taghizade S, Almasi Amin, Shahsavani B, Zand H. (2012). Creating a model of metabolic syndrome through diet in rats. Journal of University of Medical Sciences FASA. 2(4):288-296. [In Persian]
  13. Hoseini-Kakhak SA, Khaleghzadeh H, Nemati M, Hamedinia MR. (2015). The effect of combined aerobic-resistance training on lipid profile and liver
  14. enzymes in patients with non-alcoholic fatty liver under diet. Sport Physiology. 7(27):65-84. [In Persian]
  15. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. (2007). Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Preventive Cardiology. 14(6):753-760.
  16. Kondratov RV. (2007). A role of the circadian system and circadian proteins in aging. Ageing Research Reviews. 6(1):12-27.
  17. Mirghani SJ, Yousefi MS. (2015). The effect of interval recovery periods during HIIT on liver enzymes and lipid profile in overweight women. Science & Sports. 30(3):147-154.
  18. Nakhaee H, Nazarali P, Hanachi P, Hedayati M. (2018). The effect of aerobic training and Cinnamon Zeylanicum intake on total antioxidant capacity in active women. The Horizon of Medical Sciences. 24(2):88-95.
  19. Ore A, Akinloye OA. (2019). Oxidative stress and antioxidant biomarkers in clinical and experimental models of non-alcoholic fatty liver disease. Medicina. 55(2):26.
  20. Rahmani A, Gorzi A, Ghanbari M. (2019). The effects of high intensity interval training and strenuous resistance training on hippocampal antioxidant capacity and serum levels of malondialdehyde and total antioxidant capacity in male rats. SJKU. 23(6):47-58.
  21. Rapavi E, Kocsis I, Feher E, Szentmihalyi K, Lugasi A, Szekely E, Blazovics A. (2007). The effect of citrus flavonoids on the redox state of alimentary-induced fatty liver in rats. Natural Product Research. 21(3):274-281.
  22. Rasool M, Iqbal J, Malik A, Ramzan HS, Qureshi MS, Asif M, Qazi MH, Kamal MA, Chaudhary AGA, Al-Qahtani MH, Gan SH, Karim S. (2014). Hepatoprotective effects of Silybum marianum (Silymarin) and Glycyrrhiza glabra (Glycyrrhizin) in combination: a possible synergy. Evidence-Based Complementary and Alternative Medicine. 2014.
  23. Sadeghi A, Asad MR, Ferdosi MH. (2017). The effect of twelve weeks of endurance training on liver enzyme levels in obese Iranian women. Journal of Research in Sports Medicine and Technology. 15(13):49-60. [In Persian]
  24. Serce A, Toptanci BC, Tanrikut SE, Altaş S, Kizil G, Kizil S, Kizil M. (2016). Assessment of the antioxidant activity of Silybum marianum seed extract and its protective effect against DNA oxidation. protein damage and lipid peroxidation. Food Technology and Biotechnology. 54(4):455-461.
  25. Shafiee A, Gaeini A, Soleimani M, Nekouei A, Hadidi V. (2014). The effect of eight week of high intensity interval training on expression of mir-210 and ephrinA3 mRNA in soleus muscle healthy male rats. Journal of Arak University of Medical Sciences. 17(3):26-34.
  26. Steban JP, Dinani A. (2020). Lifestyle interventions beyond diet and exercise for patients with nonalcoholic fatty liver disease. Gastroenterol Hepatol. 16(3):119-130.
  27. Surai PF. (2015). Silymarin as a natural antioxidant: an overview of the current evidence and perspectives. Antioxidants. 4(1):204-247.
  28. Vona R, Gambardella L, Cittadini C, Straface E, Pietraforte D. (2019). Biomarkers of oxidative stress in metabolic syndrome and associated diseases. Oxidative Medicine and Cellular Longevity. 2019.

 

  1. Wilkinson MJ, Manoogian EN, Zadourian A, Lo H, Fakhouri S, Shoghi A, Wang X, Fleisher JG, Navlakha S, Panda S, Taub, PR. (2020). Ten-hour time-restricted eating reduces weight, blood pressure, and atherogenic lipids in patients with metabolic syndrome. Cell Metabolism 31(1):92-104.
  2. Xie F, Zhou H, Wang Y. (2019). Atherogenic index of plasma is a novel and strong predictor associated with fatty liver: a cross-sectional study in the Chinese Han population. Lipids in Health and Disease. 18(1):1-6.
  3. Yaghmaei P, Oryan S, Solati J, Mohammadi K, Salari AA. (2011). Evaluation of anxiolytic effects of silymarin extract from Silybum marianum in rats [In Persian]. Scientific Journal of Kurdistan University of Medical Sciences. 15(4):43-