نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 کارشناس ارشد فیزیولوژی ورزشی، گروه تربیت‌بدنی، واحد علی‌آبادکتول، دانشگاه آزاد اسلامی، علی‌آبادکتول، ایران
2 2دکترای فیزیولوژی ورزشی، گروه تربیت‌بدنی، واحد علی‌آبادکتول، دانشگاه آزاد اسلامی، علی‌آبادکتول، ایران
3 استادیار، گروه تربیت‌بدنی، واحد علی‌آبادکتول، دانشگاه آزاد اسلامی، علی‌آبادکتول، ایران
4 استادیار، گروه تربیت بدنی و علوم ورزشی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران
چکیده
هدف: مطالعه حاضر به بررسی تأثیر تمرین هوازی تناوبی به همراه مصرف مکمل سیلی‌مارین بر شاخص‌های بافتی آنتی‌اکسیدانی، آسیب کبدی و آتروژنیک رت‌های نر ویستار تغذیه شده با غذای پرچرب پرداخت.
روش بررسی: مطالعه حاضر از نوع تجربی و روش آزمایشگاهی بود. بدین منظور، 35 سر رت نر نژاد ویستار با سن سه هفته و وزن 08/7±45/160 گرم، به روش تصادفی به 5 گروه 7 سری شامل کنترل غذای معمولی (ODC)، کنترل غذای چرب (FDC)، غذای چرب+مکمل (FDS)، غذای چرب+تمرین (FDE)، غذای چرب+تمرین+مکمل (FDSE) تقسیم شدند. برنامه تمرینی شامل 8 هفته، 5 جلسه 30 دقیقه‌ای فعالیت روی نوارگردان در هفته بود. مکمل سیلی‌مارین با دوز 140 میلی‌گرم/کیلوگرم/روزانه نسبت به وزن بدن به مدت دو هفته گاواژ می‌‌شد. 48 ساعت پس از آخرین جیره غذایی، نمونه بافتی و خون‌گیری انجام و داده‌ها تحلیل شدند.
یافته‌ها: متغیرهایALT، AST، ALP، MDA، TC و TG در گروه‌های غذای چرب+تمرین+مکمل و غذای چرب+تمرین نسبت به گروه کنترل غذای چرب کاهش معنی‌داری داشتند. در حالی‌‌که متغیرهای SOD و HDL-C، تنها در گروه غذای چرب+تمرین+مکمل نسبت به کنترل غذای چرب افزایش معنی‌داری داشت. متغیرهای LDL-C و AIP نیز تنها در گروه غذای چرب+تمرین+مکمل نسبت به کنترل غذای چرب کاهش معنی‌داری داشتند (05/0>p).
نتیجه‌گیری: استفاده از تمرین هوازی تناوبی به تنهایی یا با مصرف مکمل سیلی‌مارین، سبب کاهش متغیرهای خطرزای بیماری کبد چرب غیرالکلی از قبیل آنزیم‌های درگیر و شاخص آتروژنیک در موش‌های تغذیه‌شده با غذای پرچرب می‌شود؛ بنابراین می-تواند از طریق افزایش عملکرد آنتی‌اکسیدانی و کاهش پروفایل چربی‌های خون، به بهبود عارضه موردنظرکمک نماید.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of 8 weeks of aerobic interval exercise with silymarin supplementation on antioxidant, liver damage and atherogenic indicators in male Wistar rats fed a high-fat diet
نویسندگان [English]
- Masoud Shakki 1
- Fatemeh Hosseini 2
- Saeed Ghorbani 3
- reza rezai shirazi 3
- H parsian 4
1 MSc in sport physiology, Department of Physical Education, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
2 PhD in sport physiology, Department of Physical Education, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
3 Assistant Professor, Department of Physical Education, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
4 Assistant Professor, Department of Physical Education and sport science, Shah-e-Qods Branch, Islamic Azad University, Tehran, Iran
چکیده [English]
Aim: The purpose of the study was to investigate the effect of 8 weeks of aerobic interval exercise with silymarin supplementation on antioxidant, liver damage and atherogenic indicators in male Wistar rats fed a high-fat diet.
Methods: This experimental study with laboratory method was performed on 35 male Wistar rats with a body weight of 160.45±7.08g and 3-weeks-of-age were randomly divided into 5 groups consisted of ordinary diet control (ODC), fatty diet control (FDC), fatty diet with supplement (FDS), fatty diet with exercise (FDE), fatty diet with supplement and exercise (FDSE). The exercises included the running on treadmill for 8 weeks, five times/week and 30 minutes in an exercise session. Silymarin supplementation with dose of 140 mg/kg/day of body, weight was received for two weeks. Liver tissue and samples were obtained after 48 hours of the last diet and data analyzed.
Results: Significant decrease in FDSE and FDE groups compared with FDC group in ALT, AST, and ALP, MDA, TC and TG variables were observed. Whereas, there were significantly increased in FDSE group compared with FDC in SOD and HDL-C variables. Also, LDL-C and AIP in FDSE group compared with FDC had shown a significant decrease (p> .05).
Conclusion: The applying of aerobic interval exercise alone or with silymarin supplementation reduced the risk to NAFLD such as enzymes involved and atherogenic index in male Wistar rats fed a high-fat diet; therefore, it could probably improve the disease through increase the antioxidant capacity and reduce blood lipid profiles.
کلیدواژه‌ها [English]
- Aerobic Interval Exercise
- Silymarin
- Antioxidant
- Atherogenic Index
- Nonalcoholic Fatty Liver
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