نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 گروه فیزیولوژی ورزشی واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
2 گروه فیزیولوژی ورزشی، واحد آیت الله آملی، دانشگاه آزاد اسلامی، آمل، ایران
چکیده
هدف: دیابت نوع 2 و چاقی با تغییرات میزان متابولیسم بدن همراه هستند که عامل تعیین کننده در پیشرفت مقاومت به انسولین محسوب می‌شود. هدف از این تحقیق بررسی تاثیر شش هفته تمرین تناوبی با شدت بالا بر میزان فاکتور رشد اندوتلیال عروقی A (VEGF-A) و پروتئین متصل به اسید چرب بافت چربی (A-FABP) در بافت چربی موش‌های صحرایی چاق مبتلا به دیابت نوع 2 بود.
روش‌شناسی: در این مطالعه تجربی، تعداد 32 سر موش صحرایی نر نژاد ویستار چاق (میانگین وزن 20±300 گرم) بهطور تصادفی در چهار گروه شامل 1) کنترل چاق، 2) شم (چاق)، 3) دیابتی چاق و 4) دیابتی چاق- تمرین تناوبی با شدت بالا قرار گرفتند. برای ایجاد دیابت نوع 2 از تزریق درون صفاقی نیکوتین آمید- استرپتوزوسین استفاده شد. برنامه تمرین تناوبی با شدت بالا 4 روز در هفته به‌مدت شش هفته اجرا شد. میزان VEGF-A و A-FABP بافت چربی رت‌ها با استفاده از کیت و به روش الایزا اندازه‌گیری شد.
یافته‌ها: میزان VEGF-A بافت چربی در گروه دیابتی چاق نسبت به گروه کنترل چاق بهطور معنی‌داری کمتر بود (001/0P=). همچنین میزان A-FABP (001/0P=) بافت چربی و مقاومت به انسولین (004/0P=) در گروه دیابتی چاق نسبت به گروه کنترل چاق بهطور معنی‌داری بیشتر بود. تمرین تناوبی با شدت بالا موجب افزایش معنی‌دار میزان VEGF-A و کاهش میزان A-FABP بافت چربی و مقاومت به انسولین موش‌های دیابتی چاق شد (001/0P=).
نتیجه‌گیری: به نظر می‌رسد تمرین تناوبی با شدت بالا می‌تواند به بهبود شاخص‌های متابولیک بافت چربی و مقاومت به انسولین موش‌های دیابتی چاق کمک کند.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The effect of six weeks high-intensity interval training on adipose tissue VEGF-A, A-FABP adipose tissue and insulin resistance in type 2 diabetic obese rats
نویسندگان [English]
- Maryam Belbasi 1
- Asieh Abbassi Daloii 1
- Ahmad Abdi 2
1 Department of sport physiology, Ayatollah amoli branch, Islamic Azad University, amol, Iran.
2 Assistant Professor, Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
چکیده [English]
Aim: Type 2 diabetes and obesity are associated with changes in the body's metabolism, which is a determining factor in the development of insulin resistance. The aim of this study was to investigate the effect of six weeks high-intensity interval training on Vascular endothelial growth factor A (VEGF-A) and Adipocyte fatty acid-binding protein (A-FABP) levels of adipose tissue in type 2 diabetic obese rats.
Method: To implementation of this experimental research, 32 obese male wistar rats (weight 320±20 gr) randomly were divided into 4 groups including obese control, shem (obese), obese diabetic, obese diabetic - high-intensity interval training. Intraperitoneal injection of nicotinamide amide-streptozotocin was used to induction type 2 diabetes. high-intensity interval training program was performed 4 days a week for six weeks. VEGF-A and A-FABP levels in rat adipose tissue were measured using by ELISA kit.
Results: the VEGF-A level in adipose tissue in the obese diabetic group was significantly lower than the obese control group (P=0.001). Also, A-FABP (P=0.001) and insulin resistance (P=0.004) was significantly higher in the obese diabetic group than the obese control groups. High-intensity interval training was associated with significant increase of VEGF-A and decrease A-FABP levels in adipose tissue and insulin resistance in obese diabetic rats (P=0.001).
Conclusion: It seems that high-intensity interval training can help improve adipose tissue metabolic parameters and insulin resistance in obese diabetic rats.
کلیدواژه‌ها [English]
- Diabetes
- Exercise
- Metabolic parameters
- Adipose tissue
Publisher: University oF Guilan Copyright © The Authors
This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
- Tsujimoto T, Kajio H. Strategies for glycemic control in nonobese and obese type 2 diabetic patients with coronary artery disease. Int J Cardiol 2019; 282:1-6.
- Al-Goblan AS, Al-Alfi MA, Khan MZ. Mechanism linking diabetes mellitus and obesity. Diabetes Metab Syndr Obes 2014; 7:587-91.
- Czech MP. Insulin action and resistance in obesity and type 2 diabetes. Nat Med 2017;23(7):804-814.
- Furuhashi M, Tuncman G, Görgün CZ, Makowski L, Atsumi G, Vaillancourt E, et al. Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2. Nature 2007; 447: 959-65.
- Haluzík MM, Anderlová K, Dolezalová R, Adamíková A, Haluzíková D, Housová J, et al. Serum adipocyte fatty acid binding protein levels in patients with type 2 diabetes mellitus and obesity: the influence of fenofibrate treatment. Physiol Res 2009; 58:93–9.
- Furuhashi M, Fucho R, Gorgun CZ, Tuncman G, Cao H, Hotamisligil GS. Adipocyte/macrophage fatty acid-binding proteins contribute to metabolic deterioration through actions in both macrophages and adipocytes in mice. J Clin Invest 2008; 118: 2640–2650.
- Kralisch S. and M. Fasshauer, Adipocyte fatty acid binding protein: a novel adipokine involved in the pathogenesis of metabolic and vascular disease?” Diabetologia, vol. 2013; 56:10–21.
- Tuncman G, Erbay E, Hom X, De Vivo I, Campos H, Rimm EB, et al. A genetic variant at the fatty acid-binding protein aP2 locus reduces the risk for hypertriglyceridemia, type 2 diabetes, and cardiovascular disease. Proc Natl Acad Sci USA 2006; 103: 6970-5
- Wu Y, Ding Y, Tanaka Y, Zhang W. Risk factors contributing to type 2 diabetes and recent advances in the treatment and prevention. Int J Med Sci 2014;11(11):1185-200.
- Roy H, Bhardwaj S, Ylä-Herttuala S. Biology of vascular endothelial growth factors. FEBS Lett 2006; 580:2879–87.
- Elias I, Franckhauser S, Bosch F. New insights into adipose tissue VEGF-A actions in the control of obesity and insulin resistance. Adipocyte 2013 Apr 1;2(2):109-12.
- Sun K, Kusminski CM, Luby-Phelps K, et al. Brown adipose tissue derived VEGF-A modulates cold tolerance and energy expenditure. Mol Metab 2014;3: 474–483
- Elmasri H, Karaaslan C, Teper Y, et al. Fatty acid binding protein 4 is a target of VEGF and a regulator of cell proliferation in endothelial cells. FASEB J 2009;23(11):3865-3873.
- Talebi Garkani E, Fathi R, Khodadadi Tirkalai S, Safarzadeh A. The effect of one session of aerobic exercise on A-FABP gene expression in visceral adipose tissue of diabetic rats. Iranian Journal of Diabetes and Metabolism. 2012; 11 (4): 358-365
- Moghadasi M, Hosseini F, Bahrami Abdehgah E, Abdollahpur N, Hosseini SA Effect of eight weeks high intensity aerobic training on adipocyte fatty acid-binding protein in obese middle-aged men Metabolism and Exercise a biannual Jounal 2013; 4 (1): 49-58. [Persian].
- Safarzade A, jafaee M, Talebi-Garakani E, Fathi R. Effects of Four Week Progressive Resistance Training on Plasma FABP4 and Lipid Profile Concentrations in Diabetic Rats. IJEM 2014; 15 (6) :538-544. [Persian].
- Czarkowska-Paczek B, Zendzian-Piotrowska M, Bartlomiejczyk I, Przybylski J, Gorski J. The influence of physical exercise on the generation of TGF-J, PDGFAA, and VEGF-A in adipose tissue. Eur J Appl Physiol 2011; 111: 875-81.
- Lee HJ. Exercise training regulates angiogenic gene expression in white adipose tissue. J Exerc Rehabil 2018;14(1):16-23.
- Disanzo BL, You T. Effects of exercise training on indicators of adipose tissue angiogenesis and hypoxia in obese rats. Metabolism 2014; 63: 452-5.
- Türk Y, Theel W, van Huisstede A, Gert-Jan M. van de Geijn, Birnie E, Short-term and long-term effect of a high-intensity pulmonary rehabilitation programme in obese patients with asthma: a randomised controlled trial Obes Sci Pract 2017; 3(3): 258–271.
- Taylor J, Keating SE, Holland DJ, Coombes JS, Leveritt MD. The Chronic Effect of Interval Training on Energy Intake: A Systematic Review and Meta-Analysis. J Obes. 2018; 2018:6903208.
- Fathi R, Ebrahimi M, Sanami SK. Effects of High Fat Diet and High Intensity Aerobic Training on Interleukin 6 Plasma Levels in Rats. Pathobiology Research 2015; 18(3):109-16. [Persian].
- Safhi MM, Anwer T, Khan G, Siddiqui R, Moni Sivakumar S, Alam MF. The combination of canagliflozin and omega-3 fatty acid ameliorates insulin resistance and cardiac biomarkers via modulation of inflammatory cytokines in type 2 diabetic rats. Physiology & Pharmacology 2018;22(5):493-501.
- Fallahi A, Gaeini A, Shekarfroush S, Khoshbaten A. Cardioprotective effect of high intensity interval training and nitric oxide metabolites (NO2−, NO3−). Iranian journal of public health 2015; 44(9):1270-1276. [Persian].
- Ghorbanzadeh V, Mohammadi M, Dariushnejad H, Chodari L, Mohaddes G. Effects of crocin and voluntary exercise, alone or combined, on heart VEGF-A and HOMA-IR of HFD/STZ induced type 2 diabetic rats. J Endocrinol Invest 2016; 39(10):1179-86. [Persian].
- Wagner H, Fischer H, Degerblad M, Alvarsson M, Gustafsson T. Improvement of insulin sensitivity in response to exercise training in type 2 diabetes mellitus is associated with vascular endothelial growth factor A expression. Diab Vasc Dis Res 2016;13(5):361-6.
- Hatano D, Ogasawara J, Endoh S, Sakurai T, Nomura S, Kizaki T, Ohno H, Komabayashi T, Izawa T. Effect of exercise training on the density of endothelial cells in the white adipose tissue of rats. Scand J Med Sci Sports 2011;21(6): e115-21.
- Bonora E. Insulin resistance as an independent risk factor for cardiovascular disease: clinical assessment and therapy approaches. Av Diabetol. 2005; 21:255–261.
- Lu X, Ji Y, Zhang L, Zhang Y, Zhang S, A Y, et al. Resistance to obesity by repression of VEGF gene expression through induction of brown-like adipocyte differentiation. Endocrinology 2012; 153:3123–32.
- Cypess AM, Lehman S, Williams G, Tal I, Rodman D, Goldfine AB, et al. Identification and importance of brown adipose tissue in adult humans. N Engl J Med 2009; 360:1509–17.
- Elmasri H, Karaaslan C, Teper Y, Ghelfi E, Weng M, Ince TA, Kozakewich H, Bischoff J, Cataltepe S. Fatty acid binding protein 4 is a target of VEGF and a regulator of cell proliferation in endothelial cells. FASEB J. 2009;23(11):3865-73.
- Xu A, Wang Y, Xu JY, Stejskal D, Tam S, Zhang J, et al. Adipocyte fatty acid binding protein is a plasma biomarker closely associated with obesity and metabolic syndrome. Clin Chem 2006; 52: 405-13
- Rodríguez-Calvo R, Girona J, Alegret JM, Bosquet A, Ibarretxe D, Masana L. Role of the fatty acid-binding protein 4 in heart failure and cardiovascular disease. J. Endocrinol 2017, 233, R173–R184.
- Kucharski M, Kaczor U. Fatty Acid Binding Protein 4 (FABP4) and the Body Lipid Balance. Folia Biologica (Kraków) 2017, 65, 181–186.
- Russell AP, Hesselink MK, Lo SK, Schrauwen P. Regulation of metabolic transcriptional coactivators and transcription factors with acute exercise. FASEB J 2005; 19(8):986-8.
- Tu WJ, Guo M, Shi XD, Cai Y, Liu Q, Fu CW. First-Trimester Serum Fatty Acid-Binding Protein 4 and Subsequent Gestational Diabetes Mellitus. Obstet. Gynecol 2017, 130, 1011–1016.
- Maeda K, Cao H, Kono K, Gorgun CZ, Furuhashi M, Uysal K, Tet al. Adipocyte/macrophage fatty acid binding proteins control integrated metabolic responses in obesity and diabetes. Cell. Metab 2005, 1, 107–119.
Talebi Garkani E, Fathi R, Khodadadi Tirklaei S, Safarzadeh A. The effect of one session of aerobic exercise on the expression of A-FABP gene in visceral adipose tissue of diabetic rats. Iranian Journal of Diabetes and Metabolism 2012; 11 (4): 358-365. [Persian].
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