نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
گروه علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران
چکیده
فشار اکسیداتیو در به وجود آمدن و عوارض ناشی از بیماری های زیادی همانند دیابت نوع دو دخیل است.با توجه به شیوع بالای دیابت در جوامع و عوارض اقتصادی وبهداشتی ناشی ازآن، تحقیق حاضر سعی دارد تا اثرتمرین استقامتی واسیدکلروژنیک را روی برخی شاخص های فشار اکسیداتیو بررسی کند. 35 سرموش نرC57BL6 4 هفته ای به صورت تصادفی دردوگروه رژیم پرچرب برای القای پیش دیابت و رژیم نرمال قرار گرفتند. پس از القای پیش دیابت پس از مصرف 12 هفته رژیم پرچرب، نمونه های این گروه به گروه‌های بدون مداخله، مصرف اسیدکلروژنیک، تمرین استقامتی، تمرین استقامتی واسیدکلروژنیک (7=n) تقسیم شدند. پروتکل تمرینی به صورت فزاینده برروی تردمیل انجام گرفت. اسیدکلروژنیک به میزان 110 میلی گرم برکیلوگرم وزن موش، سه مرتبه در هفته از طریق گاواژ به گروه‌ها داده شد. 24 ساعت پس از آخرین جلسه تمرینی و مصرف اسیدکلروژنیک، بافت برداری ازعضله اسکلتی چهارسررانی انجام شد وپس ازوزن‌کشی، برای اندازه‌گیری GPX،NO ،TAC وMDA به آزمایشگاه منتقل گردید. برای تجزیه و تحلیل داده ها از آزمون ANOVA دو راهه در سطح 05/0 استفاده شد.تمرین استقامتی موجب کاهش GPX،NO ،TAC وMDA گردید.اسیدکلروژنیک موجب کاهش GPXو NO و افزایش TACوMDA گردید و مداخله همزمان آن ها موجب افزایش GPX، TACو MDA وکاهش NO گردید.(p
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The effect of endurance training and chlorogenic acid consumption on oxidative stress markers in diabetic mices
نویسندگان [English]
- Sahar Ghasemi Pour
- Sayyed Mohammad Marandi
Department of sport sciences, University of Isfahan, Isfahan, Iran
چکیده [English]
Oxidative stress is involved in occurrence and side effects of many diseases .Considering the high prevalence of diabetes in the society and the economic and health consequences caused by it, the present study tries to evaluate the effect of endurance exercise and chlorogenic acid on some pressure indicators.35 4 weeks male C57BL6 mice were randomly assigned to two groups of high-fat diet to induce pre-diabetes and normal diet. After induction of prediabetes,after 12 weeks of high fat diet consumption the samples of this group were divided into groups without intervention, chlorogenic acid consumption, endurance training, endurance training and chlorogenic acid (n=7). The training protocol was performed incrementally . Chlorogenic acid at the rate of 110 mg per kilogram of mouse weight was given to the groups . 24 hours after the last training session and consumption of chlorogenic acid, tissue removal was done from the quadriceps skeletal muscle and after weighing, it was taken to the laboratory to measure GPX, NO, TAC and MDA. One-way ANOVA test at 0.05 level was used to analyze the data. Findings: Endurance training decreased GPX, NO, TAC, and MDA. Chlorogenic acid decreased GPX, NO, and increased TAC and MDA, and their simultaneous intervention increased GPX, TAC, and MDA and decreased NO.According to the findings of this research, it seems that endurance exercise and chlorogenic acid consumption can be used as a non-pharmacological intervention to prevent oxidative stress in the body and its complications. It can also play a preventive role in diabetic patients.
کلیدواژه‌ها [English]
- Pre diabetes
- endurance training
- colorogenic acidoxidative stress
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