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لیست داوران سال 1401

لیست داوران سال 1400

اثرات تمرین تناوبی شدید و مکمل کورکومین بر مقدار پروتئین‌های شوک گرمایی ۶۰ و ۲۰ و بیان میکرو RNA‌های ۲۱ و ۳۰ در بافت قلب موش‌های صحرایی تحت مواجهه با آرسنیک

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزشی، گروه علوم ورزشی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

2 استادیار فیزیولوژی ورزشی، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

3 دانشیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

چکیده

هدف: هدف از پژوهش حاضر بررسی اثرات تمرین تناوبی شدید و مصرف مکمل کورکومین بر مقدار پروتئین‌های شوک گرمایی و بیان ژن میکرو RNA‌های بافت قلبی موش‌های صحرایی تحت مواجهه با آرسنیک بود.
روش‌: 40 سر رت نر نژاد ویستار به طور تصادفی به چهار گروه ده‌تایی شامل کنترل، کورکومین، تمرین و توام(کورکومین+تمرین) تقسیم شدند. آرسنیک پنج میلی‌گرم بر کیلوگرم وزن بدن در روز و کورکومین 15 میلی‌گرم بر کیلوگرم وزن بدن در روز در کل دوره تحقیق به صورت خوراکی مصرف شد. تمرین تناوبی شدید به مدت هشت هفته و پنج جلسه در هفته، 60 دقیقه در هر جلسه(متشکل از 10 تناوب دویدن 4 دقیق‌های با شدت معادل 85-90 درصد vo2max و با دوره‌های استراحتی فعال 2 دقیقه‌ای) اجرا شد. مقدار پروتئین‌ و بیان ژنی به ترتیب توسط روش‌های وسترن بلات و PCR اندازه‌گیری شدند.
یافته‌ها: مقدار بیان miR-21 تفاوت بین‌گروهی نداشت(05/0<P). در هر سه گروه مداخله، مقدار پروتئین‌های HSP60، HSP20 و بیان ژن‌ miR-30 به طور معنی‌داری بیشتر از گروه کنترل بود(001/0P<). همچنین مقدار پروتئین‌های HSP60، HSP20 در گروه توام بیشتر از هر دو گروه تمرین و یا کورکومین بود(05/0P<).
نتیجه‌گیری: مواجهه با آرسنیک از طریق تغییر مقدار پروتئین‌های شوک گرمایی و نیز بیان میکروRNAها شاید سبب افزایش آسیب‌پذیری کاردیومیوسیت‌ها شود. تمرین تناوبی شدید و مصرف کورکومین هم شاید در کاهش اثرات مضر آرسنیک بر قلب مؤثر باشند، ولی مداخله توام احتمالا فواید بیشتری داشته باشد. اما به دلیل نبود شواهد تحقیقی مشابه و عدم اندازه‌گیری جامع عوامل تأثیرگذار، هنوز نیاز به انجام تحقیقات بیشتر باقی است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of High-Intensity Interval Training and Curcumin Supplementation on Cardiac Tissue HSP60 and HSP20 Levels and Gene Expression levels of miR-21 and miR-30 in Rats Exposed to Arsenic

نویسندگان [English]

  • Aghil Mahdavi 1
  • Roghayeh Pouzesh Jadidi 2
  • Karim Azali Alamdari 3

1 MSc of Exercise Physiology, Azarbaijan Shahid Madani University, Tabriz, Iran

2 Assistant Professor of Exercise Physiology, Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran

3 Associate Professor of Exercise Physiology, Azarbaijan Shahid Madani University, Tabriz, Iran

چکیده [English]

Introduction: The aim of this study was to evaluate the effects of high-intensity interval training (HIIT) and curcumin supplementation on cardiac heat shock proteins level and gene expression level of microRNAs in rats exposed to arsenic.
Methods: 32 male Wistar rats were randomly divided into four groups of Control, Curcumin, Exercise and concomitant(Curcumin+Exercise). Arsenic 5 mg/kg.day and curcumin 15 mg/kg.day, were consumed orally for entire the study period. HIIT were conducted for six weeks (5 d/w, 60 min/session (consisted of 10 intervals of running (4 min) at 85-90% of Vo2max with 2 min recovery at 50-60% of Vo2max). Tissue protein content and gene expression levels was assessed by Western blotting and PCR methods respectively. Results: No between group difference was observed for gene expression levels of miR-21(P>0.05).
The cardiac HSP60, HSP20 levels and the gene expression levels of miR-30 was significantly higher in all three intervention groups compared to Control group(P<0.001).
Moreover, the cardiac HSP60, HSP20 content was significantly higher in concomitant group compared to both groups of Exercise and Curcumin(P<0.05).
Conclusion: Arsenic exposure may likely increase the vulnerability of cardiomyocytes to injury by altering the amount of heat shock proteins content and the expression levels of microRNAs. Both HIIT and curcumin supplementation could be likely effective to reduce the hazards of arsenic within heart, However, the concomitant intervention might be had more beneficial effects. However, more research is still needed to be done due to the lack of similar evidence and no comprehensive measurement of the influential factors.

کلیدواژه‌ها [English]

  • High-Intensity Interval Training
  • Curcumin
  • Heat Shock Protein
  • MicroRNA
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سوخت و ساز و فعالیت ورزشی
دوره 12، شماره 1 - شماره پیاپی 1
فروردین 1401
صفحه 35-58
فایل ها
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  • تاریخ دریافت: 18 آبان 1401
  • تاریخ بازنگری: 21 اسفند 1401
  • تاریخ پذیرش: 22 اسفند 1401
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