نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I


1 گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

2 استادیار، گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران


هدف مطالعه حاضر تعیین تاثیر تمرین هوازی و مکمل کورکومین بر برخی شاخص‌های میتوفاژی ناشی از ایسکمی/خونرسانی مجدد کلیه موش‌های صحرایی نر بود. روش شناسی: 50 سر موش صحرائی نر (12 هفته‌ای با وزن 57/28 ± 23/315) پس از القای ایسکمی کلیه، به طور تصادفی در پنج گروه کنترل سالم، کنترل ایسکمی، تمرین، کورکومین، تمرین + کورکومین تقسیم شدند. برنامه تمرین هوازی شامل هشت هفته متشکل از پنج جلسه تمرین در هر هفته بود که هر جلسه با سرعت 10 متر در دقیقه، شیب 5 درصد به مدت 10 دقیقه در روز، آغاز شد. سرعت و زمان دویدن به تدریج تا 15 متر در دقیقه به مدت 20 دقیقه در روز افزایش یافت. در دو جلسه آخر شدت فعالیت هوازی به 25 متر در دقیقه و به مدت 30 دقیقه در روز رسید. هر جلسه دو دقیقه گرم کردن و سرد کردن با سرعت 10 متر در دقیقه انجام شد. اما نسبت به گروه تمرین معنی‌دار (به ترتیب 7/0=p و 1/0=p) نبود. نتیجه‌گیری: ایسکمی کلیوی موجب افزایش بیان ژن‌های Bnip3 و HIF-1α می شود اما به دنبال 8 هفته تمرین هوازی بیان ژن HIF-1α کاهش و بیان ژن Bnip3 افزایش یافت.



عنوان مقاله [English]

Effects of aerobic training and curcumin supplementation on some mitophagy indices induced by renal ischemia/reperfusion of male rats

نویسندگان [English]

  • Amir Dadashzadeh 1
  • Roghayeh Pouzesh Jadidi 2
  • Omid Azin Fam 1
  • Jabraeel Pouzesh Jadidi 1

1 Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran

2 : Assistant Prof., Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran

چکیده [English]

Aim: Was to investigate the effects of eight weeks aerobic training and curcumin supplementation on some mitophagy indices induced by ischemia/reperfusion of male rats. Methods: Fifty eight (age: 12 weeks, weight: 315.23 ± 28.57 gr) male rats were randomized into five groups including on Healthy control, Ischemic cotrol, Curcumin, Training, Training+Curcumin(Concomitant). Aerobic training program were conducted for eight weeks (5 d/w) starting with running at a speed of 10 m/min, 5% incline for 10 min per day. The running speed and time were gradually increased up to 15-20 m/min per day. In the last two sessions, the intensity of aerobic training reached 25 m/min for 30 min per day with 2 min recovery period at 10 m/min. curcumin (200 mg/bw.day) were consumed through oral gavage for six weeks. The gene expression levels of miR-1 and miR-133 were evaluated using Real-Time PCR method and the data were analyzed using one-way analysis of variance and Tukey post hoc test at the significance level of p <0.05. Results: In all four intervention groups, including ischemia control, exercise, curcumin and combination, the expression of HIF-1α and BNIP3 in renal tissue increased significantly (p=0.001 in all groups) compared to the control group. However, the expression of HIF-1α and BNIP3 genes in renal tissue after exercise caused a significant decrease and increase (p=0.007 and p=0.01, respectively) compared to the healthy control and ischemic control groups. Also, curcumin and Concomitant were associated with an increase in HIF-1α and BNIP3 gene expression in renal tissue

کلیدواژه‌ها [English]

  • Curcumin
  • Mitophagy
  • HIF-1α
  • BNIP3
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