نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 دانشجوی دکتری فیزیولوژی ورزشی، دانشگاه آزاد تبریز، تبریز، ایران
2 دانشیار گروه تربیت بدنی و علوم ورزشی، واحد تبریز، دانشگاه آزاد اسالمی، تبریز، ایران
3 استادیار گروه دامپزشکی، واحد تبریز، دانشگاه آزاد اسالمی، تبریز، ایران
چکیده
هدف: مطالعه حاضر با هدف تعیین دو ماه تمرین ورزشی ترکیبی بر بیان ژن هایPERK و IRE1α عضله‌ی پهن جانبی رت-های ویستار نر انجام گردید.
روش‌کار: مطالعه حاضر در قالب یک طرح تجربی سه گروهی با گروه کنترل انجام شد. 35 سر رت ویستار نر به شکل تصادفی در قالب چهار گروه کنترل، استقامتی، قدرتی، استقامتی_قدرتی جایگزین شدند و به غیر از گروه کنترل، سه گروه بعدی به مدت دو ماه در انجام تمرینات ورزشی شرکت کردند. جراحی رتها 48 ساعت پس از آخرین جلسه‌ی تمرین انجام و بیان ژن های PERK و IRE1α عضله‌ی پهن جانبی رتهای ویستار نر با استفاده از روش Real Time-PCR بررسی شد. داده‌ها با استفاده از آزمون تحلیل واریانس یک راهه در سطح معنی‌داری 05/0> P تجزیه و تحلیل شدند.
یافته‌ها: نتایج حاصل از این تحقیق نشان داد متعاقب دو ماه تمرین استقامتی بیان ژن‌های IRE1α و PERK افزایش معنی-داری نسبت به گروه کنترل داشتند (001/0p= و001/0p=). گروه تمرین مقاومتی هیچ تاثیری بر بیان ژن IRE1α نداشت (03/0p=)، ولی باعث افزایش بیان ژن PERK نسبت به گروه کنترل شد (001/0p=). همچنین گروه تمرین ترکیبی در بیان ژن IRE1α هیچ تفاوتی نسبت به گروه کنترل نداشته (62/0p=)، ولی باعث افزایش بیان ژن PERK نسبت به گروه کنترل شد (008/0p=).
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The effect of two months of combined exercise training on IRE1α and PERK gene expression in the vastus lateralis muscle of male Wistar rats.
نویسندگان [English]
- Zahra Esmaeili 1
- Farzad Zehsaz 2
- Alireza Nourazar 3
1 PhD student of exercise physiology, Tabriz Azad University, Tabriz, Iran,
2 Associate Professor of Exercise Physiology, Faculty of Human Sciences, Tabriz Azad University, Tabriz, Iran
3 Assistant Professor of Veterinary Medicine, Tabriz Azad University, Faculty of Veterinary Medicine, Tabriz, Iran
چکیده [English]
Purpose: The present study aimed to determine 2 months of combined exercise training on the expression of PERK and IRE1α genes in Wistar male rats' Vastus lateralis muscle.
Method: The present study was conducted in a three-group experimental design with control group. 35 male Wistar rats were randomly assigned to four groups of control, endurance, strength, and endurance-strength and In addition to the control group, the next three groups participated in exercise exercises for two months. 48 hours after the last training session, and expression of PERK and IRE1α genes of Wistar male rats evaluated by Real Time-PCR. Data were analyzed using one way ANOVA at a significant level of P <0.05.
Results: The results of this study showed that, after two months of endurance training, expression of IRE1α and PERK genes were significantly increased compared to the control group. Resistance training group did not have any effect on IRE1α gene expression but increased expression of PERK gene compared to control group. Also, the combined exercise group did not differ in the expression of IRE1α gene compared to the control group but increased the expression of PERK gene compared to the control group.
کلیدواژه‌ها [English]
- Combined practice
- Vastus lateralis muscle
- Apoptosis
- UPR
- ER strees
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